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Columns & Beads

Score 8.86

Omnifit Labware (Diba) Columns, Glass: BENCHMARK COLUMN 10MM/150MM 2xA

Kinesis

From
$ 579.80 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
006BCC-10-15-AA
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Columns & Beads

Score 9.85

Glass beads SKU : Z250473

Sigma-Aldrich

From
$ 56.00 (357 Units)
Sizes
1 (357 Units)
Catalog IDs
Z250473-1PAK
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Columns & Beads

Score 7.92

Steri Sterilizer replacement glass beads SKU : Z380075

Sigma-Aldrich

From
$ 37.40 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
Z380075-1PAK
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Columns & Beads

Score 9.70

ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm

Waters

From
$ 688.00 (3 Items)
Sizes
1 (3 Items)
Catalog IDs
186004623
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Columns & Beads

Score 4.36

Column tube assembly

GE Healthcare Life Sciences

From
$ 1,259.59 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
28966649
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Resins

Score 8.61

Sephadex G-100 Superfine

Sephadex

Binding Capacity
/
/
Composition
/
/
Applicable Processes
/
/
From
$ 629.00 (100 g)
Sizes
1 (100 g)
Catalog IDs
17006101
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Resins

Score 7.81

Sephadex G-75 Superfine

Sephadex

Binding Capacity
/
/
Composition
/
/
Applicable Processes
/
/
From
$ 476.00 (100 g)
Sizes
1 (100 g)
Catalog IDs
17005101
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Resins

Score 1.62

Fmoc-Val-Wang Resin

P3 Biosystems

Binding Capacity
/
/
Composition
/
/
Applicable Processes
/
/
From
$ 128.00 (25 g)
Sizes
1 (25 g)
Catalog IDs
51015-25G
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Columns & Beads

Score 9.94

MagNA Lyser Green Beads

Roche Life Science

From
$ 304.00 (100 Tubes)
Sizes
1 (100 Tubes)
Catalog IDs
03358941001
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Columns & Beads

Score 8.30

BENCHMARK MICROBORE COLUMN 3MM/150MM 2xF

Kinesis

From
$ 114.60 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
006BCC-03-15-FF
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Columns & Beads

Score 4.88

RediSep Rf Reversed-phase C18 Columns, 4.3 gram

Teledyne ISCO

From
$ 126.00 (2 Columns)
Sizes
1 (2 Columns)
Catalog IDs
69-2203-410
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Columns & Beads

Score 8.28

MEGASTREP TYPE3 XT BEAD 5% 2ML

IBA Lifesciences

From
$ 257.00 (2 ml)
Sizes
1 (2 ml)
Catalog IDs
24090002
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Spin Columns and beads have become commonplace in nucleic acid extraction; a technique which serves as the starting point of many downstream processes. In the past, extraction of DNA and RNA were performed by using ethanol, phenol, and chloroform. However, these methods use reagents and biochemicals which are hazardous. Moreover, the preparation time of extracting DNA via conventional methods are relatively slow. Now, new approaches involving spin-column and beads have been developed, allowing scientists perform extraction in a fast, more convenient way, for improved Sample Preparation.

Spin Column Nucleic Acid Purification

The principle behind spin-column extraction builds on the fact that nucleic acids bind to silica. The process of spin column-based nucleic acid purification involves four main steps, which are: cell lysis, binding, washing up and elution.

To begin, cultured cells are lysed by using a lysis buffer that contains various detergents, salts, and inhibitors. Next, binding solution is added to the lysate and transferred to the column. The lysis buffer works to remove cellular membranes which enclose the DNA, exposing it to the chaotropic agents within the binding solution. These agents work to break the hydrogen bonds within the DNA molecule, providing the conditions necessary for silica to bind to nucleic acids.

The column is then centrifuged, forcing the nucleic acids (with the binding buffer) to pass through the column which contains the silica, and the flow through the solution is then discarded. So, in principle, the nucleic acids stay within the silica membrane in the column, and the extra liquid (like binding buffer) pass through. Afterward, the washing up process is performed. Wash buffer is added to the column and centrifuged to remove impurities. Finally, the elution buffer is added, and the column is centrifuged, allowing for nucleic acids to be collected for further research.

Beads Nucleic Acid Purification

Beads are another popular option which enables scientists to isolate nucleic acid in a fast and easy way. Silica is a common material for beads, but with an added magnetic coat.

The basic principle of using magnetic beads is similar to the working principle of spin columns. The cultured cells are lysed and a binding buffer which contains chaotropic agents are added to the lysate. The addition of binding buffer to the lysate leads to binding of nucleic acids to magnetic beads. Afterward, a magnetic force is applied, and all the magnetic beads (binding with targeted molecules) are aggregated together, and the unbound molecules are left within the solution. The supernatant which contains unbound molecules are removed and the magnetic beads are resuspended with elution buffer to release the bonded molecules. Finally, magnetic force is applied to the system again to facilitate the collection of the nucleic acid.