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Columns & Beads

Omnifit Labware (Diba) Columns, Glass: BENCHMARK COLUMN 10MM/150MM 2xA

Kinesis

From
$ 579.80 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
006BCC-10-15-AA
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Columns & Beads

Glass beads SKU : Z250473

Sigma-Aldrich

From
$ 56.00 (357 Units)
Sizes
1 (357 Units)
Catalog IDs
Z250473-1PAK
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Columns & Beads

Steri Sterilizer replacement glass beads SKU : Z380075

Sigma-Aldrich

From
$ 37.40 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
Z380075-1PAK
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Columns & Beads

ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm

Waters

From
$ 688.00 (3 Items)
Sizes
1 (3 Items)
Catalog IDs
186004623
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Resins

Benzamidine Sepharose 4 Fast Flow (High Sub)

GE Healthcare Life Sciences

Binding Capacity
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Applicable Processes
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From
$ 371.00 (25 ml)
Sizes
1 (25 ml)
Catalog IDs
17512310
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Resins

Chelating Sepharose Fast Flow

GE Healthcare Life Sciences

Binding Capacity
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From
$ 418.00 (50 ml)
Sizes
2 (50 - 500 ml)
Catalog IDs
17057501, 17057502
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Resins

CNBr-Activated Sepharose 4B

GE Healthcare Life Sciences

Binding Capacity
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Composition
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Applicable Processes
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From
$ 420.00 (15 g)
Sizes
1 (15 g)
Catalog IDs
17043001
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Columns & Beads

Column tube assembly

GE Healthcare Life Sciences

From
$ 1,259.59 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
28966649
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Columns

His Buffer Kit

GE Healthcare Life Sciences

Material
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Capacity
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Pore Size
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From
$ 130.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
11003400
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Columns

His GraviTrap Kit

GE Healthcare Life Sciences

Material
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From
$ 510.00 (10 Columns)
Sizes
1 (10 Columns)
Catalog IDs
28401351
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Columns

HiScreen Capto Blue

GE Healthcare Life Sciences

Material
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Pore Size
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From
$ 205.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
28992474
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Columns

HiScreen Phenyl FF (High Sub), 4.7 ml

GE Healthcare Life Sciences

Material
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From
$ 140.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
28926988
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Spin Column Nucleic Acid Purification

The principle behind spin-column extraction builds on the fact that nucleic acids bind to silica. The process of spin column-based nucleic acid purification involves four main steps, which are: cell lysis, binding, washing up and elution.

To begin, cultured cells are lysed by using a lysis buffer that contains various detergents, salts, and inhibitors. Next, binding solution is added to the lysate and transferred to the column. The lysis buffer works to remove cellular membranes which enclose the DNA, exposing it to the chaotropic agents within the binding solution. These agents work to break the hydrogen bonds within the DNA molecule, providing the conditions necessary for silica to bind to nucleic acids.

The column is then centrifuged, forcing the nucleic acids (with the binding buffer) to pass through the column which contains the silica, and the flow through the solution is then discarded. So, in principle, the nucleic acids stay within the silica membrane in the column, and the extra liquid (like binding buffer) pass through. Afterward, the washing up process is performed. Wash buffer is added to the column and centrifuged to remove impurities. Finally, the elution buffer is added, and the column is centrifuged, allowing for nucleic acids to be collected for further research.

Beads Nucleic Acid Purification

Beads are another popular option which enables scientists to isolate nucleic acid in a fast and easy way. Silica is a common material for beads, but with an added magnetic coat.

The basic principle of using magnetic beads is similar to the working principle of spin columns. The cultured cells are lysed and a binding buffer which contains chaotropic agents are added to the lysate. The addition of binding buffer to the lysate leads to binding of nucleic acids to magnetic beads. Afterward, a magnetic force is applied, and all the magnetic beads (binding with targeted molecules) are aggregated together, and the unbound molecules are left within the solution. The supernatant which contains unbound molecules are removed and the magnetic beads are resuspended with elution buffer to release the bonded molecules. Finally, magnetic force is applied to the system again to facilitate the collection of the nucleic acid.