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Restek™ RTX™-5MS Capillary Columns, 60m or Longer: I.D. 0.25mm SKU: 12626-600
Material
Stainless Steel
Stainless Steel
Capacity
/
Pore Size
/
Scientific Score
7.49
Brand
Restek
Sizes
1 (6 Units)
Available From
1 Vendor
Price
$ 4,430.00
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Column Reactor 12 ml
Material
Stainless steel 316L
Stainless steel 316L
Capacity
12 ml
12 ml
Pore Size
20 μm
20 μm
Scientific Score
9.23
Brand
GE Healthcare Life Sciences
Sizes
1 (1 Unit)
Available From
0 Vendors
Price
Not available
Catalog IDs
-
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Brand
Waters
Sizes
1 (100 Cartridges)
Available From
1 Vendor
Price
$ 261.00
Catalog IDs
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Brand
Waters
Sizes
1 (100 Cartridges)
Available From
1 Vendor
Price
$ 199.00
Catalog IDs
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Silica Membrane Mini Spin Columns (850 µl Volume)
Material
/
Capacity
/
Pore Size
/
Scientific Score
7.88
Brand
BPI Technologies
Sizes
1 (200 Columns)
Available From
1 Vendor
Price
$ 80.00
Catalog IDs
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Silica Membrane Mini Spin Columns (650 µl Volume)
Material
/
Capacity
/
Pore Size
/
Scientific Score
7.88
Brand
BPI Technologies
Sizes
1 (200 Columns)
Available From
1 Vendor
Price
$ 70.00
Catalog IDs
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Silica Membrane Mini Spin Columns (5 ml Volume)
Material
/
Capacity
/
Pore Size
/
Scientific Score
7.88
Brand
BPI Technologies
Sizes
1 (20 Columns)
Available From
1 Vendor
Price
$ 50.00
Catalog IDs
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Silica Membrane Mini Spin Columns (30 ml Volume)
Material
/
Capacity
/
Pore Size
/
Scientific Score
8.41
Brand
BPI Technologies
Sizes
1 (20 Columns)
Available From
1 Vendor
Price
$ 60.00
Catalog IDs
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Brand
Cube Biotech
Sizes
1 (5 Cartridges)
Available From
1 Vendor
Price
$ 806.00
Catalog IDs
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Brand
Cube Biotech
Sizes
1 (5 Cartridges)
Available From
1 Vendor
Price
$ 768.00
Catalog IDs
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Brand
Cube Biotech
Sizes
1 (5 Cartridges)
Available From
1 Vendor
Price
$ 768.00
Catalog IDs
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PureCube Co-NTA Cartridge (5x5 ml)
Scientific Score
8.18
Brand
Cube Biotech
Sizes
1 (5 Cartridges)
Available From
1 Vendor
Price
$ 640.00
Catalog IDs
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Spin columns are a staple piece of plastic-ware used to purify and cleanup nucleic acids, (such as DNA/RNA) or proteins, directly from cultured cells. The columns usually contain some quantity of silica; known to bind free nucleic acids. Columns have been developed to be cheap, quick and efficient at removing multiple contaminants.

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Main Steps of Spin Column Use

Lysis – Cells are broken up to release nucleic acids.
Binding – The addition of the lysed sample to a buffer solution and ethanol/isopropanol forms a binding solution. This solution is pipetted into the column and centrifuged, forcing the solution through the silica membrane, to which the nucleic acids bind.
Washing – After centrifugation, the flow-through liquid is discarded and wash buffer is pipetted into the column and centrifuged, so as to remove any impurities.
Elution – After disposing of the wash buffer, an elution buffer is added and centrifuged. The nucleic acid then collects in the follow-through liquid and can be stored or used for downstream research purposes.