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Columns

Omnifit Labware (Diba) Accessories: REPLACEMENT GLASS 3MM/100MM

Diba Perfecting Fluidics

From
$ 90.98 (1 Item)
Sizes
1 (1 Item)
Catalog IDs
006RG-03-10
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Columns

Plastic Spin Columns

Agarose Bead Technologies

From
$ 129.60 (25 Columns)
Sizes
1 (25 Columns)
Catalog IDs
SP-25
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Empty FPLC 8ml Columns

Agarose Bead Technologies

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$ 77.10 (3 Columns)
Sizes
1 (3 Columns)
Catalog IDs
FPLC8-3
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8 ml FLEXI-Column™

Agarose Bead Technologies

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$ 82.50 (2 Columns)
Sizes
1 (2 Columns)
Catalog IDs
FLEC8-2
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Prepacked Columns Sepadextran™-25 Medium SC

Agarose Bead Technologies

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$ 273.44 (50 Columns)
Sizes
1 (50 Columns)
Catalog IDs
SCSD25M-50
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Column Wrap for HiPrep 16 Column

GE Healthcare Life Sciences

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$ 21.00 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
28902150
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HiScreen Capto Phenyl ImpRes, 4.7 ml, 4.7 ml

GE Healthcare Life Sciences

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$ 208.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
17548410
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Columns

SUPELCOSIL™ LC-18 HPLC Column SKU : 58298

Supelco

Material
/
/
Capacity
/
/
Pore Size
120 Å
120 Å
From
$ 646.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
58298
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Columns

SUPELCOSIL™ LC-NH2 HPLC Column SKU : 58338

Supelco

Material
/
/
Capacity
/
/
Pore Size
120 Å
120 Å
From
$ 646.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
58338
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Columns

SUPELCOSIL™ LC-18 HPLC Column SKU : 59209

Supelco

Material
/
/
Capacity
/
/
Pore Size
120 Å
120 Å
From
$ 489.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
59209
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Columns

SUPELCOSIL™ ABZ+Plus HPLC Column SKU : 59197c40

Supelco

Material
/
/
Capacity
/
/
Pore Size
120 Å
120 Å
From
$ 817.00 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
59197C40
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Columns

Sephadex® G-50 SKU : g5050

Sigma-Aldrich

Material
/
/
Capacity
/
/
Pore Size
/
/
From
$ 110.00 (10 g)
Sizes
3 (10 - 100 g)
Catalog IDs
G5050-10G, G5050-50G, ...
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Spin columns are a staple piece of plastic-ware used to purify and cleanup nucleic acids, (such as DNA/RNA) or proteins, directly from cultured cells. The columns usually contain some quantity of silica; known to bind free nucleic acids. Columns have been developed to be cheap, quick and efficient at removing multiple contaminants.

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Main Steps of Spin Column Use

Lysis – Cells are broken up to release nucleic acids.
Binding – The addition of the lysed sample to a buffer solution and ethanol/isopropanol forms a binding solution. This solution is pipetted into the column and centrifuged, forcing the solution through the silica membrane, to which the nucleic acids bind.
Washing – After centrifugation, the flow-through liquid is discarded and wash buffer is pipetted into the column and centrifuged, so as to remove any impurities.
Elution – After disposing of the wash buffer, an elution buffer is added and centrifuged. The nucleic acid then collects in the follow-through liquid and can be stored or used for downstream research purposes.