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Brand
Whatman
Sizes
1 (1000 µl)
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1 Vendor
Price
$ 191.68
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Brand
G-Biosciences
Sizes
1 (500000 µl)
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1 Vendor
Price
$ 58.27
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Brand
Moravek
Sizes
1 (1000 µCi)
Available From
1 Vendor
Price
$ 442.90
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Brand
Moravek
Sizes
1 (5000 µCi)
Available From
1 Vendor
Price
$ 901.25
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Brand
Moravek
Sizes
1 (1000 µCi)
Available From
1 Vendor
Price
$ 442.90
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Brand
Moravek
Sizes
1 (5000 µCi)
Available From
1 Vendor
Price
$ 901.25
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L-FMAU, [2-13C, 1,3-15N] ≥98% (by HPLC) MORpure™
Material
/
Capacity
/
Pore Size
/
Brand
Moravek
Sizes
1 (1000 µg)
Available From
1 Vendor
Price
$ 804.23
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Thiourea, [14C] ≥98% (by HPLC) MORpure™
Material
/
Capacity
/
Pore Size
/
Brand
Moravek
Sizes
1 (5000 µCi)
Available From
1 Vendor
Price
$ 3,400.00
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PMEG ≥98% (by HPLC) MORpure™
Material
/
Capacity
/
Pore Size
/
Brand
Moravek
Sizes
2 (1000 - 5000 µg)
Available From
1 Vendor
Price Range
$ 175.10 - $ 468.65
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HPMPA(S) ≥98% (by HPLC) MORpure™
Material
/
Capacity
/
Pore Size
/
Brand
Moravek
Sizes
2 (1000 - 5000 µg)
Available From
1 Vendor
Price Range
$ 326.92 - $ 863.08
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D-FMAU ≥98% (by HPLC) MORpure™
Material
/
Capacity
/
Pore Size
/
Brand
Moravek
Sizes
1 (1000 µg)
Available From
1 Vendor
Price
$ 797.69
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Silica Flour
Material
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Capacity
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Pore Size
/
Brand
Univar Usa
Sizes
1 (453.592 g)
Available From
1 Vendor
Price
$ 369.24
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Spin columns are a staple piece of plastic-ware used to purify and cleanup nucleic acids, (such as DNA/RNA) or proteins, directly from cultured cells. The columns usually contain some quantity of silica; known to bind free nucleic acids. Columns have been developed to be cheap, quick and efficient at removing multiple contaminants.

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Main Steps of Spin Column Use

Lysis – Cells are broken up to release nucleic acids.
Binding – The addition of the lysed sample to a buffer solution and ethanol/isopropanol forms a binding solution. This solution is pipetted into the column and centrifuged, forcing the solution through the silica membrane, to which the nucleic acids bind.
Washing – After centrifugation, the flow-through liquid is discarded and wash buffer is pipetted into the column and centrifuged, so as to remove any impurities.
Elution – After disposing of the wash buffer, an elution buffer is added and centrifuged. The nucleic acid then collects in the follow-through liquid and can be stored or used for downstream research purposes.