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Columns
Omnifit Labware (Diba) Accessories: REPLACEMENT GLASS 3MM/100MM
Scientific Score
9.18
Brand
Diba Perfecting Fluidics
Sizes
1 (1 Item)
Available From
1 Vendor
Price
$ 90.98
Catalog IDs
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Columns
Plastic Spin Columns
Scientific Score
4.48
Brand
Agarose Bead Technologies
Sizes
1 (25 Columns)
Available From
1 Vendor
Price
$ 129.60
Catalog IDs
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Columns
Empty FPLC 8ml Columns
Scientific Score
4.48
Brand
Agarose Bead Technologies
Sizes
1 (3 Columns)
Available From
1 Vendor
Price
$ 77.10
Catalog IDs
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Columns
Prepacked Columns Sepadextran™-25 Medium SC
Scientific Score
4.48
Brand
Agarose Bead Technologies
Sizes
1 (50 Columns)
Available From
1 Vendor
Price
$ 273.44
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Columns
RTX™-65TG Capillary Columns
Material
Stainless Steel
Stainless Steel
Capacity
/
/
Pore Size
/
/
Scientific Score
8.16
Brand
Restek
Sizes
5 (1 Unit)
Available From
2 Vendors
Price Range
$ 367.71 - $ 656.29
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Columns
R&D Systems™ Column Rack
Scientific Score
4.28
Brand
R&D Systems
Sizes
1 (1 Unit)
Available From
1 Vendor
Price
$ 189.00
Catalog IDs
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Columns
5 μL On-column Syringe Metal Plunger 0.47 mm OD Needle
Scientific Score
0.88
Brand
Perkin Elmer
Sizes
1 (1 Item)
Available From
1 Vendor
Price
$ 126.00
Catalog IDs
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Columns
SPE Supra-Clean SI-S (Silica) Column, 1 g/6 ml
Scientific Score
2.07
Brand
Perkin Elmer
Sizes
1 (30 Columns)
Available From
1 Vendor
Price
$ 81.00
Catalog IDs
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Columns
Guard Column Holder
Scientific Score
2.07
Brand
Perkin Elmer
Sizes
1 (1 Item)
Available From
1 Vendor
Price
$ 697.00
Catalog IDs
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Columns
Elite-5 capillary column, 30m x 0.32mm, 0.25µm
Scientific Score
1.59
Brand
Perkin Elmer
Sizes
1 (1 Column)
Available From
1 Vendor
Price
$ 612.00
Catalog IDs
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Columns
Elite 624 capillary column 30m x 0.25mm, 1.40µm
Scientific Score
1.59
Brand
Perkin Elmer
Sizes
1 (1 Item)
Available From
1 Vendor
Price
$ 613.00
Catalog IDs
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Columns
Elite 624 capillary column 60m x 0.32mm, 1.80µm
Scientific Score
1.59
Brand
Perkin Elmer
Sizes
1 (1 Item)
Available From
1 Vendor
Price
$ 1,200.00
Catalog IDs
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Spin columns are a staple piece of plastic-ware used to purify and cleanup nucleic acids, (such as DNA/RNA) or proteins, directly from cultured cells. The columns usually contain some quantity of silica; known to bind free nucleic acids. Columns have been developed to be cheap, quick and efficient at removing multiple contaminants.

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Main Steps of Spin Column Use

Lysis – Cells are broken up to release nucleic acids.
Binding – The addition of the lysed sample to a buffer solution and ethanol/isopropanol forms a binding solution. This solution is pipetted into the column and centrifuged, forcing the solution through the silica membrane, to which the nucleic acids bind.
Washing – After centrifugation, the flow-through liquid is discarded and wash buffer is pipetted into the column and centrifuged, so as to remove any impurities.
Elution – After disposing of the wash buffer, an elution buffer is added and centrifuged. The nucleic acid then collects in the follow-through liquid and can be stored or used for downstream research purposes.