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High Pure RNA Isolation Kit
Targets
Total RNA
Total RNA
Sample Type
Blood, Cells, Culture
Blood, Cells, Culture
Time to Sample
25 min
25 min
Scientific Score
9.94
Brand
Roche Life Science
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 259.00
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RNA/DNA/Protein Purification 96-Well Kit
Targets
Genomic DNA, Proteins, Total RNA, miRNA
Genomic DNA, Proteins, Total RNA, miRNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
45 min
45 min
Scientific Score
8.07
Brand
Norgen Biotek
Sizes
1 (96 Reactions)
Available From
1 Supplier
Price
$ 703.00
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Plasma/Serum RNA Purification Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
15-20 min
15-20 min
Scientific Score
7.22
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 345.00
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Sputum DNA Isolation Kit
Targets
Bacterial DNA, Genomic DNA
Bacterial DNA, Genomic DNA
Sample Type
Sputum
Sputum
Time to Sample
< 60 min
< 60 min
Scientific Score
7.24
Brand
Norgen Biotek
Sizes
1 (25 Reactions)
Available From
1 Supplier
Price
$ 142.00
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FFPE RNA/DNA Purification Plus Kit
Targets
Genomic DNA, Total RNA, miRNA
Genomic DNA, Total RNA, miRNA
Sample Type
FFPE tissue
FFPE tissue
Time to Sample
70-160 min
70-160 min
Scientific Score
7.55
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 427.00
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GenElute™ mRNA Miniprep Kit
Starting Material
RNA
RNA
Final Product
mRNA
mRNA
Organism
Animals, Human
Animals, Human
Scientific Score
9.64
Brand
Sigma-Aldrich
Sizes
1 (10 Reactions)
Available From
2 Suppliers
Price Range
$ 149.15 - $ 159.00
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Food Oil DNA Isolation Kit
Targets
Genomic DNA
Genomic DNA
Sample Type
Food
Food
Time to Sample
25 min
25 min
Scientific Score
6.28
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 205.00
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GenElute™ RNA/DNA/Protein Purification Plus Kit
Targets
Genomic DNA, Proteins, Total RNA
Genomic DNA, Proteins, Total RNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
< 30 min
< 30 min
Scientific Score
8.70
Brand
Sigma-Aldrich
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 615.00
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NucleoSpin® 8
Targets
Genomic DNA
Genomic DNA
Sample Type
Cells, Culture, Tissue
Cells, Culture, Tissue
Time to Sample
20 min
20 min
Scientific Score
9.27
Brand
Macherey-Nagel
Sizes
2 (96 - 480 Reactions)
Available From
1 Supplier
Price Range
$ 308.00 - $ 1,325.00
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High Pure PCR Cleanup Micro Kit
Input Type
Gel, PCR reaction
Gel, PCR reaction
Targets
Genomic DNA, cDNA
Genomic DNA, cDNA
Removal
Dimers, Salts, Small molecules, Unincorporated nucleotides, Unincorporated primers
Dimers, Salts, Small molecules, Unincorporated nucleotides, Unincorporated primers
Scientific Score
8.84
Brand
Roche Life Science
Sizes
2 (50 - 200 Reactions)
Available From
1 Supplier
Price Range
$ 120.00 - $ 412.00
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Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
35-40 min
35-40 min
Scientific Score
6.17
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 1,494.00
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NucleoSpin® 8 Plant II Core Kit
Targets
Genomic DNA
Genomic DNA
Sample Type
Tissue
Tissue
Time to Sample
60 min
60 min
Scientific Score
8.92
Brand
Macherey-Nagel
Sizes
1 (384 Reactions)
Available From
1 Supplier
Price
$ 855.00
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Obtaining a pure sample for testing, whether it is DNA, RNA or protein usually comprises of two parts: extraction followed by cleanup. Common technologies used include silica membrane spin columns, magnetic beads and solutions such as Trizol and TRI Reagent.

Extraction to Preservation

Extraction

Extraction initially isolates the nucleic acid or peptide of interest from its source; this can be tissue, cells or a lysate, but to name a few. Check out our protein purification or plasmid purification troubleshooting guides for guidance.

Cleanup

Cleanup is then employed to remove any remaining contaminants from the final sample, therefore increasing the concentration of the molecule of interest. Cleanup is of particular importance for electrophoresis and PCR. Our DNA Gel Cleanup troubleshoot can provide you with solutions to any problems you encounter.

Enrichment

Enrichment aims to amplify the concentration of a small subset of the target molecule, usually a species which is difficult to acquire in significant volume. Don’t be afraid to make use of our sample enrichment troubleshoot when problems arise. Also, see our RNA enrichment troubleshooting guide, when attempting to deplete ribosomal RNA or enrich mRNA, (through poly-A enrichment).

Preservation

Preservation is usually a final step of sample purification, used to maintain the activity and structure of the sample for future testing. Any technique used needs to prevent degradation, especially from enzymes such as RNase or DNase.