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Extraction & Cleanup on ZAGENO

ZR Fungal/Bacterial DNA MiniPrep™ Zymo Research
Sample Amount 10^7-10^9 cells, 50-200 mg tissue Technology BashingBead, Fast-Spin column
From $ 170.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6005
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Plant DNA Isolation Kit (Magnetic Bead System) Norgen Biotek
Sample Amount 50 mg Technology Magnetic bead system
From $ 134.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 58200
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Micro Bio-Spin P-30 Gel Columns, Tris Buffer (RNase-free) Bio-Rad
Columns or Beads Columns Technology / Applicable Processes Sample cleanup
From $ 137.00 (25 Columns)
Sizes 2 (25 - 100 Columns)
Catalog IDs 7326250, 7326251
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iPrep™ ChargeSwitch® Buccal Cell Kit Invitrogen
Sample Amount 1-5 punched-out circles from dried blood (5mm diameter), 8 mm cigarette butt, hair follicles Technology ChargeSwitch technology, magnetic bead-based technology
From $ 240.23 (52 Preparations)
Sizes 1 (52 Preparations)
Catalog IDs IS-10003
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TRI Reagent® LS Sigma-Aldrich
Sample Amount 250 µl biological fluid, 5-10 x 10^6 cells Technology Guanidine thiocyanate and phenol in a monophase solution
From $ 229.00 (100 ml)
Sizes 2 (100 - 200 ml)
Catalog IDs T3934-100ML, T3934-200ML
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NucleoMag® 96 Plant Macherey-Nagel
Sample Amount 20-50 mg tissue Technology Magnetic-bead technology
From $ 183.00 (96 Preparations)
Sizes 3 (96 - 2304 Preparations)
Catalog IDs 744400.1, 744400.4, ...
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polyA Spin™ mRNA Isolation Kit New England Biolabs
Control Type / Starting Material Total RNA Sample Amount 1 mg total RNA, 100-1000 µg tissue, 5 x 10^7 cells
From $ 216.00 (8 Isolations)
Sizes 1 (8 Isolations)
Catalog IDs S1560S
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Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues Invent Biotechnologies
Target Protein Total protein Sample Amount 20-500 µg Preparation Method Purification
From $ 198.00 (50 Preparations)
Sizes 2 (4 - 50 Preparations)
Catalog IDs SD-001_SN-002-2, SD-001_SN-002-1
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ZR-96 DNA Clean-up Kit™ (Shallow well format) Zymo Research
Sample Amount / Technology Fast-Spin, Silicon-A™ Plate
From $ 220.00 (192 Reactions)
Sizes 2 (192 - 384 Reactions)
Catalog IDs D4017, D4018
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Aurum Affi-Gel Blue Mini Kit Bio-Rad
Sample Amount 500 µl Technology Spin-column format
From $ 82.00 (2 Reactions)
Sizes 1 (2 Reactions)
Catalog IDs 7326712
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DNA Gel Extraction Kit Norgen Biotek
Sample Amount < 400 mg Technology Spin column chromatography
From $ 91.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs 13100
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NucleoMag® 96 Tissue Macherey-Nagel
Sample Amount < 20 mg human / animal tissue Technology Magnetic-bead technology
From $ 286.00 (96 Preparations)
Sizes 3 (96 - 2304 Preparations)
Catalog IDs 744300.1, 744300.4, ...
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Obtaining a pure sample for testing, whether it is DNA, RNA or protein usually comprises of two parts: extraction followed by cleanup. Common technologies used include silica membrane spin columns, magnetic beads and solutions such as Trizol and TRI Reagent.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Extraction to Preservation

Extraction

Extraction initially isolates the nucleic acid or peptide of interest from its source; this can be tissue, cells or a lysate, but to name a few. Check out our protein purification or plasmid purification troubleshooting guides for guidance.

Cleanup

Cleanup is then employed to remove any remaining contaminants from the final sample, therefore increasing the concentration of the molecule of interest. Cleanup is of particular importance for electrophoresis and PCR. Our DNA Gel Cleanup troubleshoot can provide you with solutions to any problems you encounter.

Enrichment

Enrichment aims to amplify the concentration of a small subset of the target molecule, usually a species which is difficult to acquire in significant volume. Don’t be afraid to make use of our sample enrichment troubleshoot when problems arise. Also, see our RNA enrichment troubleshooting guide, when attempting to deplete ribosomal RNA or enrich mRNA, (through poly-A enrichment).

Preservation

Preservation is usually a final step of sample purification, used to maintain the activity and structure of the sample for future testing. Any technique used needs to prevent degradation, especially from enzymes such as RNase or DNase.