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Extraction & Cleanup on Zageno

DynaMag™-96 Side Skirted Magnet Invitrogen
Target Protein / Sample Amount / Preparation Method /
From Not available
Sizes 1 (1 Magnet)
Catalog IDs 12027
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PureLink® miRNA Isolation Kit Invitrogen
Extraction of / Sample Type / Time to Sample /
From Not available
Sizes 1 (25 Preparations)
Catalog IDs K157001
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miRCURY RNA Isolation Kit - Cell and Plant Exiqon
Extraction of Total RNA Sample Type Cells, Plant tissue Time to Sample /
From $ 295.45 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 300110
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XBridge BEH C18 Column, 130Å, 3.5 μm Waters
Columns or Beads / Targets / Size Range /
From Not available
Sizes 1 (1 Column)
Catalog IDs 186003034
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Glass beads, acid-washed 425-600 µm (30-40 U.S. sieve) MilliporeSigma
Columns or Beads / Targets / Size Range /
From $ 124.66 (100 g)
Sizes 1 (100 g)
Catalog IDs G8772-100G
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XK 50/30 Column GE Healthcare
Columns or Beads / Targets / Size Range /
From $ 1,125.00 (1 column)
Sizes 1 (1 column)
Catalog IDs 28988953
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ZR Fungal/Bacterial DNA MiniPrep™ Zymo Research
Extraction of Genomic DNA, Viral DNA, Mitochondrial DNA Sample Type Cells, Culture, Tissue Time to Sample 10 min
From $ 170.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6005
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Plant DNA Isolation Kit (Magnetic Bead System) Norgen Biotek
Extraction of Genomic DNA Sample Type Cells, Tissue Time to Sample 50 min / 12 purifications
From $ 134.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 58200
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Micro Bio-Spin P-30 Gel Columns, Tris Buffer (RNase-free) Bio-Rad
Columns or Beads Columns Targets Genomic DNA, Proteins, Total RNA Size Range > 22 bp, 40 kDa
From $ 130.00 (25 Columns)
Sizes 2 (25 - 100 Columns)
Catalog IDs 7326250, 7326251
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QIAamp 96 Virus QIAcube HT Kit QIAGEN
Extraction of Viral DNA, Viral RNA Sample Type Blood, Body fluids, Feces, Plasma & serum, Tissue Time to Sample 90-145 min
From $ 1,434.00 (480 Preparations)
Sizes 1 (480 Preparations)
Catalog IDs 57731
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MagAttract DNA Blood Midi M48 Kit QIAGEN
Extraction of Genomic DNA Sample Type Blood Time to Sample < 330 min
From $ 842.00 (192 Preparations)
Sizes 1 (192 Preparations)
Catalog IDs 951356
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iPrep™ ChargeSwitch® Buccal Cell Kit Invitrogen
Extraction of Genomic DNA Sample Type Buccal & saliva Time to Sample 40 min
From $ 484.00 (52 Preparations)
Sizes 1 (52 Preparations)
Catalog IDs IS-10003
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Obtaining a pure sample for testing, whether it is DNA, RNA or protein usually comprises of two parts: extraction followed by cleanup. Common technologies used include silica membrane spin columns, magnetic beads and solutions such as Trizol and TRI Reagent.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Extraction to Preservation

Extraction

Extraction initially isolates the nucleic acid or peptide of interest from its source; this can be tissue, cells or a lysate, but to name a few. Check out our protein purification or plasmid purification troubleshooting guides for guidance.

Cleanup

Cleanup is then employed to remove any remaining contaminants from the final sample, therefore increasing the concentration of the molecule of interest. Cleanup is of particular importance for electrophoresis and PCR. Our DNA Gel Cleanup troubleshoot can provide you with solutions to any problems you encounter.

Enrichment

Enrichment aims to amplify the concentration of a small subset of the target molecule, usually a species which is difficult to acquire in significant volume. Don’t be afraid to make use of our sample enrichment troubleshoot when problems arise. Also, see our RNA enrichment troubleshooting guide, when attempting to deplete ribosomal RNA or enrich mRNA, (through poly-A enrichment).

Preservation

Preservation is usually a final step of sample purification, used to maintain the activity and structure of the sample for future testing. Any technique used needs to prevent degradation, especially from enzymes such as RNase or DNase.