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DNA & RNA Extraction Kits on Zageno

PureLink® miRNA Isolation Kit Invitrogen
Extraction of / Sample Type / Time to Sample /
From Not available
Sizes 1 (25 Preparations)
Catalog IDs K157001
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miRCURY RNA Isolation Kit - Cell and Plant Exiqon
Extraction of Total RNA Sample Type Cells, Plant tissue Time to Sample /
From $ 295.45 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 300110
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ZR Fungal/Bacterial DNA MiniPrep™ Zymo Research
Extraction of Genomic DNA, Viral DNA, Mitochondrial DNA Sample Type Cells, Culture, Tissue Time to Sample 10 min
From $ 170.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6005
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Plant DNA Isolation Kit (Magnetic Bead System) Norgen Biotek
Extraction of Genomic DNA Sample Type Cells, Tissue Time to Sample 50 min / 12 purifications
From $ 134.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 58200
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QIAamp 96 Virus QIAcube HT Kit QIAGEN
Extraction of Viral DNA, Viral RNA Sample Type Blood, Body fluids, Feces, Plasma & serum, Tissue Time to Sample 90-145 min
From $ 1,434.00 (480 Preparations)
Sizes 1 (480 Preparations)
Catalog IDs 57731
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MagAttract DNA Blood Midi M48 Kit QIAGEN
Extraction of Genomic DNA Sample Type Blood Time to Sample < 330 min
From $ 842.00 (192 Preparations)
Sizes 1 (192 Preparations)
Catalog IDs 951356
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iPrep™ ChargeSwitch® Buccal Cell Kit Invitrogen
Extraction of Genomic DNA Sample Type Buccal & saliva Time to Sample 40 min
From $ 484.00 (52 Preparations)
Sizes 1 (52 Preparations)
Catalog IDs IS-10003
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MasterPure™ Yeast RNA Purification Kit Epicentre
Extraction of Total RNA Sample Type Culture, Tissue Time to Sample 80 min
From $ 307.00 (100 Preparations)
Sizes 1 (100 Preparations)
Catalog IDs MPY03100
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EndoFree Plasmid Maxi Kit QIAGEN
Extraction of Plasmid DNA Sample Type Culture Time to Sample 150 min
From $ 341.00 (10 Preparations)
Sizes 1 (10 Preparations)
Catalog IDs 12362
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ZR-96 Quick-RNA™ Zymo Research
Extraction of Total RNA Sample Type Buccal & saliva, Cells, Culture, Plasma & serum, Tissue Time to Sample 30 min
From $ 588.00 (192 Preparations)
Sizes 2 (192 - 384 Preparations)
Catalog IDs R1052, R1053
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RNeasy Maxi Kit QIAGEN
Extraction of Total RNA Sample Type Blood, Cells, Culture, Tissue Time to Sample 60 min
From $ 346.00 (12 Preparations)
Sizes 1 (12 Preparations)
Catalog IDs 75162
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RNeasy Plus Mini Kit QIAGEN
Extraction of Total RNA Sample Type Cells, Tissue Time to Sample 25 min
From $ 343.00 (50 Preparations)
Sizes 2 (50 - 250 Preparations)
Catalog IDs 74134, 74136
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DNA & RNA Extraction Function & Applications

DNA Extraction and RNA extraction are key to acquiring deoxyribonucleic acid / ribonucleic acid from cell or virus samples. It is important to know that purification, extraction, and isolation interchangeable terms but can refer to slightly different processes. Purification usually relates to eliminating contaminants, while isolation is removing as much of the target as physically possible. Extraction can combine both of these terms or be similar to isolation.

DNA extraction is vital to the downstream processes of PCR, sequencing, and genotyping, while RNA extraction is key for gene expression studies, gene knockdown, gene silencing processes and transcriptome analysis. However, isolating RNA is a meticulous process due to the presence of RNases within cells. These enzymes require inhibiting as they can rapidly degrade RNA and reduce the overall yield.

Both DNA & RNA extraction are vital first steps for preparing the final sample.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells
  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants
  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell
  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For instance, this comparison shows:

  • that the High Pure RNA Isolation kit from Roche Life Science is the cheapest per preperation
  • that the Qiaamp RNA Blood Mini Kit from Qiagen has the greatest binding capacity
  • that the Total RNA Purification Kit from Norgen Biotek can also be used to extract mRNA and miRNA

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for
your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Videos:

MrSimpleScience/YouTube

ChangBenson/YouTube