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DNA & RNA Extraction Kits

5Prime Phase Lock Gel™, PLG Light

Quantabio

Targets
Genomic DNA, Plasmid DNA, Total RNA, Viral DNA
Genomic DNA, Plasmid DNA, Total RNA, Viral DNA
Sample Type
Cells, Culture
Cells, Culture
Time to Sample
/
/
From
$ 171.88 (200 Tubes)
Sizes
1 (200 Tubes)
Catalog IDs
2302820
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DNA & RNA Extraction Kits

HigherPurity™ Circulating DNA Purification Kit

Canvax Biotech

From
$ 99.00 (20 Reactions)
Sizes
2 (20 - 50 Reactions)
Catalog IDs
AN0260-S, AN0260
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DNA & RNA Extraction Kits

HigherPurity™ Blood DNA Extraction Mini Spin Kit

Canvax Biotech

From
$ 69.00 (20 Reactions)
Sizes
3 (20 - 100 Reactions)
Catalog IDs
AN0044-S, AN0044, ...
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DNA & RNA Extraction Kits

HigherPurity™ Blood/Cultured Cell Total RNA Kit

Canvax Biotech

From
$ 178.00 (50 Reactions)
Sizes
2 (50 - 100 Reactions)
Catalog IDs
AN0145, AN0146
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DNA & RNA Extraction Kits

HigherPurity™ Blood DNA Extraction Kit (5 mL or 10 mL)

Canvax Biotech

From
$ 99.00 (100 ml)
Sizes
1 (100 ml)
Catalog IDs
AN0049
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DNA & RNA Extraction Kits

HigherPurity™ Tissue DNA Extraction Kit (Reagent based)

Canvax Biotech

From
$ 109.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
AN0052
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DNA & RNA Extraction Kits

EXTRACTME TOTAL RNA KIT

BLIRT

Targets
/
/
Sample Type
/
/
Time to Sample
/
/
From
$ 115.81 (10 Preparations)
Sizes
3 (10 - 250 Preparations)
Catalog IDs
EM09.1-010, EM09.1-050, ...
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DNA & RNA Extraction Kits

innuSPEED Bacteria/Fungi DNA Kit

Analytik Jena

From
$ 244.99 (50 Reactions)
Sizes
2 (50 - 250 Reactions)
Catalog IDs
845-KS-1510050, 845-KS-1510250
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DNA & RNA Extraction Kits

innuSPEED Soil DNA Kit

Analytik Jena

Targets
/
/
Sample Type
/
/
Time to Sample
/
/
From
$ 244.99 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
845-KS-1580050
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DNA & RNA Extraction Kits

quickAmp™ Tissue PCR Kit

ACTGene

From
$ 192.03 (100 Reactions)
Sizes
2 (100 - 1000 Reactions)
Catalog IDs
A1500-100RXN, A1500-1000RXN
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DNA & RNA Extraction Kits

Quick-DNA™ Miniprep Kit - Capped column

Zymo Research

Targets
Genomic DNA
Genomic DNA
Sample Type
Biological fluids, Blood, Buccal & saliva, Cells, Plasma & serum, Tissue
Biological fluids, Blood, Buccal & saliva, Cells, Plasma & serum, Tissue
Time to Sample
/
/
From
$ 98.00 (50 Preparations)
Sizes
2 (50 - 200 Preparations)
Catalog IDs
D3024, D3025
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DNA & RNA Extraction Kits

Fast DNA-spin™ Plasmid DNA Purification Kit

iNtRON

Targets
/
/
Sample Type
/
/
Time to Sample
/
/
From
$ 180.00 (200 Columns)
Sizes
1 (200 Columns)
Catalog IDs
17013
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DNA & RNA Extraction Function & Applications

DNA Extraction and RNA extraction are key to acquiring deoxyribonucleic acid / ribonucleic acid from cell or virus samples. It is important to know that purification, extraction, and isolation interchangeable terms but can refer to slightly different processes. Purification usually relates to eliminating contaminants, while isolation is removing as much of the target as physically possible. Extraction can combine both of these terms or be similar to isolation.

DNA extraction is vital to the downstream processes of PCR, sequencing, and genotyping, while RNA extraction is key for gene expression studies, gene knockdown, gene silencing processes and transcriptome analysis. However, isolating RNA is a meticulous process due to the presence of RNases within cells. These enzymes require inhibiting as they can rapidly degrade RNA and reduce the overall yield.

Both DNA & RNA extraction are vital first steps for preparing the final sample.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells

  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants

  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell

  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Videos:

MrSimpleScience/YouTube

ChangBenson/YouTube