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DNA & RNA Extraction Kits on ZAGENO

ZR Fungal/Bacterial DNA MiniPrep™ Zymo Research
Sample Amount 10^7-10^9 cells, 50-200 mg tissue Technology BashingBead, Fast-Spin column
From $ 170.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6005
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Plant DNA Isolation Kit (Magnetic Bead System) Norgen Biotek
Sample Amount 50 mg Technology Magnetic bead system
From $ 134.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 58200
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iPrep™ ChargeSwitch® Buccal Cell Kit Invitrogen
Sample Amount 1-5 punched-out circles from dried blood (5mm diameter), 8 mm cigarette butt, hair follicles Technology ChargeSwitch technology, magnetic bead-based technology
From $ 240.23 (52 Preparations)
Sizes 1 (52 Preparations)
Catalog IDs IS-10003
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TRI Reagent® LS Sigma-Aldrich
Sample Amount 250 µl biological fluid, 5-10 x 10^6 cells Technology Guanidine thiocyanate and phenol in a monophase solution
From $ 229.00 (100 ml)
Sizes 2 (100 - 200 ml)
Catalog IDs T3934-100ML, T3934-200ML
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NucleoMag® 96 Plant Macherey-Nagel
Sample Amount 20-50 mg tissue Technology Magnetic-bead technology
From $ 183.00 (96 Preparations)
Sizes 3 (96 - 2304 Preparations)
Catalog IDs 744400.1, 744400.4, ...
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NucleoMag® 96 Tissue Macherey-Nagel
Sample Amount < 20 mg human / animal tissue Technology Magnetic-bead technology
From $ 286.00 (96 Preparations)
Sizes 3 (96 - 2304 Preparations)
Catalog IDs 744300.1, 744300.4, ...
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ZR-96 Quick-RNA™ Zymo Research
Sample Amount 10^6 cells, 5 mg tissue Technology Fast-Spin Plate
From $ 588.00 (192 Preparations)
Sizes 2 (192 - 384 Preparations)
Catalog IDs R1052, R1053
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ZR Soil Microbe DNA MicroPrep™ Zymo Research
Sample Amount 0.25 g Technology BashingBead, Fast-Spin column
From $ 216.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6003
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Urine Exfoliated Cell RNA Purification Kit Norgen Biotek
Sample Amount 1-50 ml, 100-10^6 cells Technology Spin column chromatography
From $ 191.00 (25 Preparations)
Sizes 1 (25 Preparations)
Catalog IDs 22550
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ZymoPURE™ Plasmid Gigaprep Kit Zymo Research
Sample Amount < 2500 ml Technology Spin Column
From $ 462.00 (5 Preparations)
Sizes 1 (5 Preparations)
Catalog IDs D4204
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ZR Genomic DNA™-Tissue MicroPrep Zymo Research
Sample Amount 5000 μg Technology Fast-Spin column
From $ 119.00 (50 Preparations)
Sizes 2 (50 - 200 Preparations)
Catalog IDs D3040, D3041
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illustra bacteria genomicPrep Mini Spin Kit GE Healthcare Life Sciences
Sample Amount < 2 x 10^9 cells Technology silica-based membrane, Spin column
From $ 259.00 (50 Preparations)
Sizes 2 (50 - 250 Preparations)
Catalog IDs 28-9042-58, 28-9042-59
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DNA & RNA Extraction Function & Applications

DNA Extraction and RNA extraction are key to acquiring deoxyribonucleic acid / ribonucleic acid from cell or virus samples. It is important to know that purification, extraction, and isolation interchangeable terms but can refer to slightly different processes. Purification usually relates to eliminating contaminants, while isolation is removing as much of the target as physically possible. Extraction can combine both of these terms or be similar to isolation.

DNA extraction is vital to the downstream processes of PCR, sequencing, and genotyping, while RNA extraction is key for gene expression studies, gene knockdown, gene silencing processes and transcriptome analysis. However, isolating RNA is a meticulous process due to the presence of RNases within cells. These enzymes require inhibiting as they can rapidly degrade RNA and reduce the overall yield.

Both DNA & RNA extraction are vital first steps for preparing the final sample.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells

  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants

  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell

  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

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MrSimpleScience/YouTube

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