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High Pure RNA Isolation Kit
Targets
Total RNA
Total RNA
Sample Type
Blood, Cells, Culture
Blood, Cells, Culture
Time to Sample
25 min
25 min
Scientific Score
9.94
Brand
Roche Life Science
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 259.00
Catalog IDs
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RNA/DNA/Protein Purification 96-Well Kit
Targets
Genomic DNA, Proteins, Total RNA, miRNA
Genomic DNA, Proteins, Total RNA, miRNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
45 min
45 min
Scientific Score
8.07
Brand
Norgen Biotek
Sizes
1 (96 Reactions)
Available From
1 Supplier
Price
$ 703.00
Catalog IDs
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Plasma/Serum RNA Purification Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
15-20 min
15-20 min
Scientific Score
7.22
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 345.00
Catalog IDs
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Sputum DNA Isolation Kit
Targets
Bacterial DNA, Genomic DNA
Bacterial DNA, Genomic DNA
Sample Type
Sputum
Sputum
Time to Sample
< 60 min
< 60 min
Scientific Score
7.24
Brand
Norgen Biotek
Sizes
1 (25 Reactions)
Available From
1 Supplier
Price
$ 142.00
Catalog IDs
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FFPE RNA/DNA Purification Plus Kit
Targets
Genomic DNA, Total RNA, miRNA
Genomic DNA, Total RNA, miRNA
Sample Type
FFPE tissue
FFPE tissue
Time to Sample
70-160 min
70-160 min
Scientific Score
7.55
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 427.00
Catalog IDs
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Food Oil DNA Isolation Kit
Targets
Genomic DNA
Genomic DNA
Sample Type
Food
Food
Time to Sample
25 min
25 min
Scientific Score
6.28
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 205.00
Catalog IDs
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GenElute™ RNA/DNA/Protein Purification Plus Kit
Targets
Genomic DNA, Proteins, Total RNA
Genomic DNA, Proteins, Total RNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
< 30 min
< 30 min
Scientific Score
8.70
Brand
Sigma-Aldrich
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 615.00
Catalog IDs
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NucleoSpin® 8
Targets
Genomic DNA
Genomic DNA
Sample Type
Cells, Culture, Tissue
Cells, Culture, Tissue
Time to Sample
20 min
20 min
Scientific Score
9.27
Brand
Macherey-Nagel
Sizes
2 (96 - 480 Reactions)
Available From
1 Supplier
Price Range
$ 308.00 - $ 1,325.00
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Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
35-40 min
35-40 min
Scientific Score
6.17
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 1,494.00
Catalog IDs
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NucleoSpin® 8 Plant II Core Kit
Targets
Genomic DNA
Genomic DNA
Sample Type
Tissue
Tissue
Time to Sample
60 min
60 min
Scientific Score
8.92
Brand
Macherey-Nagel
Sizes
1 (384 Reactions)
Available From
1 Supplier
Price
$ 855.00
Catalog IDs
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NucleoSpin® RNA
Targets
Total RNA
Total RNA
Sample Type
Cells, Culture, Tissue
Cells, Culture, Tissue
Time to Sample
35 min
35 min
Scientific Score
9.22
Brand
Macherey-Nagel
Sizes
4 (10 - 250 Reactions)
Available From
1 Supplier
Price Range
$ 55.90 - $ 1,185.00
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NucleoSpin® RNA Virus F
Targets
Viral DNA, Viral RNA
Viral DNA, Viral RNA
Sample Type
Cell-free sample, Plasma & serum
Cell-free sample, Plasma & serum
Time to Sample
45 min
45 min
Scientific Score
8.33
Brand
Macherey-Nagel
Sizes
1 (25 Reactions)
Available From
1 Supplier
Price
$ 280.00
Catalog IDs
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DNA & RNA Extraction Function & Applications

DNA Extraction and RNA extraction are key to acquiring deoxyribonucleic acid / ribonucleic acid from cell or virus samples. It is important to know that purification, extraction, and isolation interchangeable terms but can refer to slightly different processes. Purification usually relates to eliminating contaminants, while isolation is removing as much of the target as physically possible. Extraction can combine both of these terms or be similar to isolation.

DNA extraction is vital to the downstream processes of PCR, sequencing, and genotyping, while RNA extraction is key for gene expression studies, gene knockdown, gene silencing processes and transcriptome analysis. However, isolating RNA is a meticulous process due to the presence of RNases within cells. These enzymes require inhibiting as they can rapidly degrade RNA and reduce the overall yield.

Both DNA & RNA extraction are vital first steps for preparing the final sample.

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DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells

  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants

  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell

  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

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