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DNA & RNA Extraction Kits

iPrep™ ChargeSwitch™ Forensic Kit

Invitrogen

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From
$ 428.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
IS10002
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DNA & RNA Extraction Kits

iPrep™ ChargeSwitch™ gDNA Tissue Kit

Invitrogen

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Technology
/
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From
$ 426.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
IS10004
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DNA & RNA Extraction Kits

PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit

Invitrogen

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From
$ 347.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
A31232
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DNA & RNA Extraction Kits

MagMAX™ CORE Nucleic Acid Purification Kit

Applied Biosystems

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From
$ 426.00 (1 Pack)
Sizes
2 (1 Pack)
Catalog IDs
A32700, A32702
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DNA & RNA Extraction Kits

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Invitrogen

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Technology
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From
$ 355.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
AM1975
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DNA & RNA Extraction Kits

PureLink™ HQ Mini Plasmid DNA Purification Kit

Invitrogen

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Technology
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From
$ 423.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
K210001
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DNA & RNA Extraction Kits

PureLink™ HiPure Plasmid Maxiprep Kit

Invitrogen

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From
$ 235.00 (1 Pack)
Sizes
2 (1 Pack)
Catalog IDs
K210006, K210007
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DNA & RNA Extraction Kits

PureLink™ HiPure Plasmid Filter Midiprep Kit

Invitrogen

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From
$ 302.00 (1 Pack)
Sizes
2 (1 Pack)
Catalog IDs
K210014, K210015
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DNA & RNA Extraction Kits

PureLink™ Total RNA Blood Kit

Invitrogen

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Technology
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From
$ 352.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
K156001
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DNA & RNA Extraction Kits

RNA isolation kit

Sigma-Aldrich

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Technology
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From
$ 587.76 (1 Each)
Sizes
1 (1 Each)
Catalog IDs
83913-1EA
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DNA & RNA Extraction Kits

GENELUTE HP PLASMID MAXIPREP KIT

Sigma-Aldrich

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From
$ 135.42 (1 Kit)
Sizes
3 (1 Kit)
Catalog IDs
NA0300-1KT, NA0300S-1KT, ...
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DNA & RNA Extraction Kits

EZ-10 Spin Column Plasmid DNA Min-preps Kit

Bio Basic

Sample Amount
1.5-5 ml
1.5-5 ml
Technology
Spin column chromatography
Spin column chromatography
From
$ 63.00 (100 Preparations)
Sizes
2 (100 - 250 Preparations)
Catalog IDs
BS414.SIZE.100preps, BS614.SIZE.250preps
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DNA & RNA Extraction Function & Applications

DNA Extraction and RNA extraction are key to acquiring deoxyribonucleic acid / ribonucleic acid from cell or virus samples. It is important to know that purification, extraction, and isolation interchangeable terms but can refer to slightly different processes. Purification usually relates to eliminating contaminants, while isolation is removing as much of the target as physically possible. Extraction can combine both of these terms or be similar to isolation.

DNA extraction is vital to the downstream processes of PCR, sequencing, and genotyping, while RNA extraction is key for gene expression studies, gene knockdown, gene silencing processes and transcriptome analysis. However, isolating RNA is a meticulous process due to the presence of RNases within cells. These enzymes require inhibiting as they can rapidly degrade RNA and reduce the overall yield.

Both DNA & RNA extraction are vital first steps for preparing the final sample.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells

  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants

  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell

  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Videos:

MrSimpleScience/YouTube

ChangBenson/YouTube