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Protein Purification

Bacterial Total Protein Extraction Reagent SKU: BWR1012

Biospes

From
$ 80.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
BWR1012-10ML
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Protein Purification

Thrombin Cleavage Capture Kit

Novagen

From
$ 323.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
69022-3
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Protein Purification

ProteinExt™ Mammalian Membrane Protein Extraction Kit

TransGen Biotech

Target Protein
/
/
Sample Type
/
/
Sample Amount
/
/
From
$ 215.38 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
DE301-01
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Protein Purification

Probe DNA Purification Kit - Column SKU: 10666

Cepham Life Sciences

Target Protein
/
/
Sample Type
/
/
Sample Amount
/
/
From
$ 278.30 (100 Columns)
Sizes
1 (100 Columns)
Catalog IDs
10666
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Protein Purification

Protino® 96 Ni-IDA

Macherey-Nagel

Target Protein
Tagged protein
Tagged protein
Sample Type
Cells, Culture
Cells, Culture
Sample Amount
20-75 mg
20-75 mg
From
$ 155.00 (96 Preparations)
Sizes
2 (96 - 384 Preparations)
Catalog IDs
745300.1, 745300.4
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Protein Purification

Score 0.80

Minute™ Nuclear Envelope Protein Extraction Kit

Invent Biotechnologies

Target Protein
Nuclear protein
Nuclear protein
Sample Type
Cells
Cells
Sample Amount
10-20 x 10^6 cells
10-20 x 10^6 cells
From
$ 398.00 (50 Preparations)
Sizes
2 (4 - 50 Preparations)
Catalog IDs
NE-013-2, NE-013-1
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Protein Purification

Score 0.80

His-Affinity Gel

Zymo Research

Target Protein
Tagged protein
Tagged protein
Sample Type
Protein solution
Protein solution
Sample Amount
/
/
From
$ 190.00 (14 ml)
Sizes
1 (14 ml)
Catalog IDs
P2003-2
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Protein Purification

InGel™ Blue

G-Biosciences

Target Protein
Total protein
Total protein
Sample Type
Protein solution
Protein solution
Sample Amount
1-2 mm gel cubes
1-2 mm gel cubes
From
$ 190.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
786-681
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Protein Purification

InGel™ Silver

G-Biosciences

Target Protein
Total protein
Total protein
Sample Type
Protein solution
Protein solution
Sample Amount
1-2 mm gel cubes
1-2 mm gel cubes
From
$ 203.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
786-241
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Protein Purification

Score 1.70

GST Fusion Protein Purification Kit

GenScript

Target Protein
Tagged protein
Tagged protein
Sample Type
Cells
Cells
Sample Amount
/
/
From
$ 139.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
L00207
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Protein Purification

Score 0.80

PureCube Ni-NTA Agarose

Cube Biotech

Target Protein
Tagged protein
Tagged protein
Sample Type
Lysate
Lysate
Sample Amount
/
/
From
$ 14.00 (1 ml)
Sizes
4 (1 - 250 ml)
Catalog IDs
31101, 31103, ...
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Protein Purification

PureCube Amine Activated Agarose

Cube Biotech

Target Protein
Other protein
Other protein
Sample Type
/
/
Sample Amount
/
/
From
$ 118.00 (10 ml)
Sizes
3 (10 - 250 ml)
Catalog IDs
51003, 51005, ...
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

His-tag and GST-tag

His-tag and GST-tag protein fusions are commonly used for heterologous protein isolation from E.coli BL21. However, when expressing a eukaryotic protein in prokaryotic systems, you might experience protein degradation and protein precipitation.In this case, or if you plan on examining post translational protein modifications such as protein phosphorylation, you should consider a eukaryotic expression system. While this results in protein modifications that are closer to those of native proteins, they might give some problems when used in combination with some purification tags.

Check our Protein Purification Troubleshoot for tips on perfecting your experiment.

Protein Size

Protein size is also something to consider. The relative size of your tag of choice compared to the protein might interfere with protein folding. For example, it is not advised to use Glutathione S-transferase (GST) fusion for the isolation of small protein for this reason. An alternative would be to use immunoaffinity chromatography, which does not require tagged proteins and can be used for natural sources.

Explore our article: Malaria - Humanity's Deadliest Predator, for more information on the research uses of Protein Enrichment Kits.

Buffer

The buffer used in extraction and purification might also interfere with the protein function or protein assays. High detergent concentration can interfere with protein folding which affects protein activity or protein-protein binding. This can be problematic when determining protein concentration, in particular using the Bradford method.

Video and image credits:

Video: David Johnson, PhD/YouTube