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Protein G - Agarose Antibody Purification Kit
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
6.28
Brand
Expedeon
Sizes
1 (2 ml)
Available From
1 Vendor
Price
$ 399.00
Catalog IDs
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ABpure Antibody Purification Kit SKU: 260-0010
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
6.52
Brand
Expedeon
Sizes
1 (1 Unit)
Available From
1 Vendor
Price
$ 599.00
Catalog IDs
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ABpure Mouse Antibody Purification Kit SKU: 261-0010
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
6.52
Brand
Expedeon
Sizes
1 (1 Unit)
Available From
1 Vendor
Price
$ 649.00
Catalog IDs
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AntibodySelect G System Antibody Purification Kit
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
5.77
Brand
Expedeon
Sizes
2 (1 Pack)
Available From
1 Vendor
Price
$ 849.00
Catalog IDs
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Antibody Concentration and Clean Up
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
6.39
Brand
Expedeon
Sizes
1 (3 Columns)
Available From
1 Vendor
Price
$ 279.00
Catalog IDs
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AntibodySelect Antibody Purification Kit
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
6.30
Brand
Expedeon
Sizes
2 (1 Pack)
Available From
1 Vendor
Price Range
$ 299.00 - $ 599.00
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g-linked GTP agarose resin
Target Protein
/
Sample Type
/
Sample Amount
/
Scientific Score
5.32
Brand
Expedeon
Sizes
2 (2 - 5 ml)
Available From
1 Vendor
Price Range
$ 799.00 - $ 1,499.00
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Protino® 96 Ni-IDA
Target Protein
Tagged protein
Tagged protein
Sample Type
Cells, Culture
Cells, Culture
Sample Amount
20-75 mg
20-75 mg
Scientific Score
9.13
Brand
Macherey-Nagel
Sizes
2 (96 - 384 Preparations)
Available From
1 Vendor
Price Range
$ 159.00 - $ 565.00
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Minute™ Yeast Mitochondria Enrichment Kit
Target Protein
Total protein
Total protein
Sample Type
Culture
Culture
Sample Amount
30-40 μl wet pellet
30-40 μl wet pellet
Scientific Score
9.20
Brand
Invent Biotechnologies
Sizes
1 (50 Preparations)
Available From
1 Vendor
Price
$ 288.00
Catalog IDs
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PROTEIN PRECIPITATION KIT
Target Protein
Total protein
Total protein
Sample Type
Protein solution
Protein solution
Sample Amount
/
Scientific Score
8.69
Brand
AMRESCO
Sizes
1 (1 Kit)
Available From
2 Vendors
Price Range
$ 83.40 - $ 106.44
Catalog IDs
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Detergent-Free Total Protein Isolation Kit
Target Protein
Total protein
Total protein
Sample Type
Cells, Culture, Tissue
Cells, Culture, Tissue
Sample Amount
50 mg tissue, 10^7-10^10 cells
50 mg tissue, 10^7-10^10 cells
Scientific Score
9.63
Brand
Norgen Biotek
Sizes
1 (25 Preparations)
Available From
1 Vendor
Price
$ 233.00
Catalog IDs
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Yeast Protein Extraction Buffer Kit
Target Protein
Total protein
Total protein
Sample Type
Culture
Culture
Sample Amount
0.1-10 ml
0.1-10 ml
Scientific Score
9.88
Brand
GE Healthcare Life Sciences
Sizes
1 (1 Kit)
Available From
2 Vendors
Price Range
$ 160.00 - $ 175.19
Catalog IDs
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Protein purification and isolation can be some of the hardest challenges in the field of biotechnology and life sciences. At the beginning you have to decide if you need the protein in a biologically active (native) or inactive state for your studies. Next, to choose the best purification technology you should know if your protein is membrane-bonded or free circulating in the cell. Then you have to determine the best environmental conditions e.g. salt concentration, pH of the buffer used to stabilize the structure of the protein.

Proteins are often tagged e.g. with a His-tag to easily purify the protein and boost the purification yield. However, they can also be purified by their size, charge and solubility, but to name a few.

Picking the right protein purification kit will depend on the protein type (species and location in the organism), your downstream application, and will also influence your cloning strategy, if working with fusion proteins.

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His-tag and GST-tag

His-tag and GST-tag protein fusions are commonly used for heterologous protein isolation from E.coli BL21. However, when expressing a eukaryotic protein in prokaryotic systems, you might experience protein degradation and protein precipitation.In this case, or if you plan on examining post translational protein modifications such as protein phosphorylation, you should consider a eukaryotic expression system. While this results in protein modifications that are closer to those of native proteins, they might give some problems when used in combination with some purification tags.

Check our Protein Purification Troubleshoot for tips on perfecting your experiment.

Protein Size

Protein size is also something to consider. The relative size of your tag of choice compared to the protein might interfere with protein folding. For example, it is not advised to use Glutathione S-transferase (GST) fusion for the isolation of small protein for this reason. An alternative would be to use immunoaffinity chromatography, which does not require tagged proteins and can be used for natural sources.

Explore our article: Malaria - Humanity's Deadliest Predator, for more information on the research uses of Protein Enrichment Kits.

Buffer

The buffer used in extraction and purification might also interfere with the protein function or protein assays. High detergent concentration can interfere with protein folding which affects protein activity or protein-protein binding. This can be problematic when determining protein concentration, in particular using the Bradford method.

Video and image credits:

Video: David Johnson, PhD/YouTube