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Protein Purification

FFPE Protein Extraction Kit

Bio Basic

Target Protein
Total protein
Total protein
Sample Amount
2 x 100 mm^2 area sections with 10-15 µm thickness
2 x 100 mm^2 area sections with 10-15 µm thickness
Preparation Method
Purification
Purification
From
$ 56.93 (50 Preparations)
Sizes
1 (50 Preparations)
Catalog IDs
BSP058
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Protein Purification

MultiMACS DYKDDDDK Isolation Kit (12×8)

Miltenyi Biotec

From
$ 1,040.00 (96 Isolations)
Sizes
1 (96 Isolations)
Catalog IDs
130-101-621
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Protein Purification

µMACS Anti-DYKDDDDK Starting Kit

Miltenyi Biotec

From
$ 915.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
130-101-636
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Protein Purification

µMACS HA Isolation Kit

Miltenyi Biotec

From
$ 530.00 (40 Isolations)
Sizes
1 (40 Isolations)
Catalog IDs
130-091-122
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Protein Purification

µMACS GST Isolation Kit

Miltenyi Biotec

From
$ 530.00 (40 Isolations)
Sizes
1 (40 Isolations)
Catalog IDs
130-091-370
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Protein Purification

µMACS VitalVirus HIV Isolation Kit

Miltenyi Biotec

From
$ 420.00 (20 Isolations)
Sizes
1 (20 Isolations)
Catalog IDs
130-092-805
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Protein Purification

Mitochondria Isolation Kit, mouse tissue

Miltenyi Biotec

From
$ 680.00 (25 Separations)
Sizes
1 (25 Separations)
Catalog IDs
130-096-946
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Protein Purification

PLANT FRACTIONATED PROTEIN EXTRACTION KI

Sigma-Aldrich

Target Protein
/
/
Sample Amount
/
/
Preparation Method
/
/
From
$ 549.35 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
PE0240-1KT
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Protein Purification

ProBond™ Purification System

Invitrogen

Target Protein
/
/
Sample Amount
/
/
Preparation Method
/
/
From
$ 564.00 (1 Pack)
Sizes
1 (1 Pack)
Catalog IDs
K85001
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Protein Purification

Minute™ Total Protein Extraction Kit for Animal Cultured Cells and Tissues

Invent Biotechnologies

Target Protein
Total protein
Total protein
Sample Amount
20-500 µg
20-500 µg
Preparation Method
Purification
Purification
From
$ 198.00 (50 Preparations)
Sizes
2 (4 - 50 Preparations)
Catalog IDs
SD-001_SN-002-2, SD-001_SN-002-1
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Protein Purification

Score 0.80

Protino® 96 Ni-NTA

Macherey-Nagel

Target Protein
Tagged protein
Tagged protein
Sample Amount
< 750 µl/well
< 750 µl/well
Preparation Method
Tag
Tag
From
$ 195.00 (96 Preparations)
Sizes
2 (96 - 384 Preparations)
Catalog IDs
745425.1, 745425.4
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Protein Purification

Score 2.50

ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit

Norgen Biotek

Target Protein
Cytoplasmatic protein, High abundance protein, Other protein
Cytoplasmatic protein, High abundance protein, Other protein
Sample Amount
100 ml
100 ml
Preparation Method
Purification
Purification
From
$ 149.00 (4 Preparations)
Sizes
1 (4 Preparations)
Catalog IDs
17700
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

His-tag and GST-tag

His-tag and GST-tag protein fusions are commonly used for heterologous protein isolation from E.coli BL21. However, when expressing a eukaryotic protein in prokaryotic systems, you might experience protein degradation and protein precipitation.In this case, or if you plan on examining post translational protein modifications such as protein phosphorylation, you should consider a eukaryotic expression system. While this results in protein modifications that are closer to those of native proteins, they might give some problems when used in combination with some purification tags.

Check our Protein Purification Troubleshoot for tips on perfecting your experiment.

Protein Size

Protein size is also something to consider. The relative size of your tag of choice compared to the protein might interfere with protein folding. For example, it is not advised to use Glutathione S-transferase (GST) fusion for the isolation of small protein for this reason. An alternative would be to use immunoaffinity chromatography, which does not require tagged proteins and can be used for natural sources.

Explore our article: Malaria - Humanity's Deadliest Predator, for more information on the research uses of Protein Enrichment Kits.

Buffer

The buffer used in extraction and purification might also interfere with the protein function or protein assays. High detergent concentration can interfere with protein folding which affects protein activity or protein-protein binding. This can be problematic when determining protein concentration, in particular using the Bradford method.

Compare Protein Purification Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For instance, this comparison shows:

  • that the Minute Plasma Membrane Protein Isolation Kit kit from Invent Biotechnologies is the cheapest per preparation

  • that the Readyprep Protein Extraction Kit from Bio-Rad accepts samples from the widest range of organsims.

  • that the Focus Subcell Kit from G-Biosciences can extract different types of protein.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Video: David Johnson, PhD/YouTube