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Gene Editing Controls
Accell Non-targeting Control Pool
Control Type
Negative
Negative
Organism
Animals, Human
Animals, Human
Applicable Processes
/
/
Scientific Score
10.00
Brand
Dharmacon
Sizes
3 (5 - 50 nmol)
Available From
1 Vendor
Price Range
$ 228.00 - $ 902.00
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Gene Editing Controls
GIPZ EG5 Lentiviral shRNA Positive Control (viral particles)
Control Type
Positive
Positive
Organism
Human
Human
Applicable Processes
/
/
Scientific Score
9.99
Brand
Dharmacon
Sizes
1 (25 µl)
Available From
1 Vendor
Price
$ 324.00
Catalog IDs
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Gene Editing Controls
GIPZ GAPDH Lentiviral shRNA Positive Control (glycerol stock)
Control Type
Positive
Positive
Organism
Animals, Human
Animals, Human
Applicable Processes
/
/
Scientific Score
9.97
Brand
Dharmacon
Sizes
1 (1 Stock)
Available From
1 Vendor
Price
$ 435.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
miRIDIAN microRNA Hairpin Inhibitor Negative Control #2, 5 nmol
Scientific Score
0.10
Brand
Dharmacon
Sizes
1 (5 nmol)
Available From
1 Vendor
Price
$ 225.00
Catalog IDs
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Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09395
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09385
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09384
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09377
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09364
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09362
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09360
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item
Gene Editing Controls
MISSION® 3′UTR Lenti GoClone™ SKU : HUTR09354
Control Type
/
/
Organism
/
/
Applicable Processes
/
/
Scientific Score
4.89
Brand
Sigma-Aldrich
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
Added to comparison remove item

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Good gene editing controls are a critical tool for troubleshooting and ensuring that the transfer worked according to plan, especially due to the high sensitivity of gene transfer experiments. RNA interference (RNAi) and reporter gene experiments assess the activity and effects of certain genes. These techniques require controls to test transfection efficiency, knockdown efficiency (RNAi), and whether conditions are still optimal (reporter genes).

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Gene Transfer Controls

Gene transfer introduces specific genetic sequences into a new host cell. This process tests whether overexpression of genetic material alters cellular functions, or if the expression of a particular gene is possible in a certain cellular system.

When choosing your gene transfer control, there are a few factors to consider. For instance, there is a difference in procedure between bacterial and mammalian cells.

When transforming bacteria, it is common to perform an additional transformation with a control vector. Vectors such as the pUC18 plasmid or the pUC19 vector are normally used for such cases. These cloning vectors, given their multiple cloning and restriction sites, can easily introduce foreign DNA into the plasmid. Antibiotics can be used as a control for transformed bacteria. This is done with a small plasmid which carries an appropriate antibiotic resistance. In addition to this, the X-gal system provides an extra layer of control by color-coding successfully transformed colonies that carry the right insert. One key example of this process is the blue-white screening technique, as seen with the pUC19 plasmid system.

Antiobiotics can also be used as controls for transformed mammalian cells. However, cells growing on antibiotics might display physiological differences that could influence your experiments. An alternative gene transfer control involves vectors which express a fluorescent protein alongside with the gene of interest. This can also be used for co-transfection of two vectors, using multiple fluorescent proteins, or for automatic selection of successful transfection.

Reporter Gene / siRNA Controls

Reporter control siRNAs ensure high reporter gene knockdown of genes such as GFP and luciferase, which indicate gene expression. Knockdown of these genes allows you to visualise when conditions are suboptimal and need to be changed; this is indicated by test samples fading to a similar level of the control.

RNAi controls are able to fully inhibit your RNA of choice or leave it completely unaffected, and therefore give a measure of the efficacy of your technique of inhibition.

Compare Gene Editing Controls

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.