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Gene Modification Kits on ZAGENO

Diversify™ PCR Random Mutagenesis Kit Clontech
Organism / Efficiency / Time to Sample >40 min
From $ 640.00 (30 Reactions)
Sizes 1 (30 Reactions)
Catalog IDs 630703
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EZ DNA Methylation™ Kit Zymo Research
Organism Animals, Human Efficiency > 99 % Time to Sample 12-16 h
From $ 138.00 (50 Reactions)
Sizes 2 (50 - 200 Reactions)
Catalog IDs D5001, D5002
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Cells-to-CpG™ Bisulfite Conversion Kit Applied Biosystems
Organism / Efficiency / Time to Sample 180 min
From $ 350.00 (50 Reactions)
Sizes 2 (50 - 192 Reactions)
Catalog IDs 4445555, 4445554
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Mutation Generation System Kit Thermo Scientific
Organism Bacteria Efficiency / Time to Sample /
From $ 232.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs F701
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EZ-96 DNA Methylation-Lightning™ Kit Zymo Research
Organism / Efficiency > 99.5 % Time to Sample 110 min
From $ 442.00 (192 Reactions)
Sizes 2 (192 Reactions)
Catalog IDs D5032, D5033
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Transformer™ Site-Directed Mutagenesis Kit Clontech
Organism Bacteria Efficiency >70–90% Time to Sample /
From $ 473.00 (30 Reactions)
Sizes 1 (30 Reactions)
Catalog IDs 630702
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TrueMethyl Whole-Genome Cambridge Epigenetix
Organism / Efficiency / Time to Sample 10 hrs
From $ 3,000.00 (48 Reactions)
Sizes 1 (48 Reactions)
Catalog IDs TrueMethyl Whole-Genome
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Q5® Site-Directed Mutagenesis Kit New England Biolabs
Organism Bacteria Efficiency / Time to Sample < 120 min
From $ 188.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs E0554S
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EZ-96 DNA Methylation™ MagPrep Zymo Research
Organism Animals, Human Efficiency > 99 % Time to Sample 40 h
From $ 601.00 (384 Reactions)
Sizes 2 (384 - 764 Reactions)
Catalog IDs D5040, D5041
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EZ RNA Methylation™ Kit Zymo Research
Organism Human Efficiency > 99% Non-methylated C residues are converted to U with > 99% Protection of 5-methylcytosine Time to Sample 90 min
From $ 189.00 (50 Preparations)
Sizes 2 (50 - 200 Preparations)
Catalog IDs R5001, R5002
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EZ DNA Methylation-Direct™ Kit Zymo Research
Organism Animals, Human Efficiency > 99.5 % Time to Sample 240 min
From $ 193.00 (50 Reactions)
Sizes 2 (50 - 200 Reactions)
Catalog IDs D5020, D5021
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TrueMethyl Array Cambridge Epigenetix
Organism / Efficiency / Time to Sample 4.5 hrs
From $ 1,500.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs TrueMethyl Array
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Gene Modification Application

Gene Modification refers to numerous gene editing techniques that can introduce mutations into double stranded DNA. Whilst the CRISPR-Cas9 system induces site-specific mutation in vivo, other genetic engineering techniques can introduce such changes during your sub-cloning steps or in vitro. Such gene modification techniques can also be used to change the specificity of RNA interference approaches.

Site Specific Mutagenesis

Site-specific mutagenesis (SSM) inserts, deletes, or substitutes a single base within the DNA sequence of a plasmid. Doing so allows for the investigation of the biological activity of selected genes and proteins.

SSM procedure starts with the synthesis of a short DNA primer, which contains your desired mutation. This synthetic primer should also be complementary to its DNA template strand so that it can hybridize with the gene of interest. Next, this primer is extended via a DNA polymerase, which inadvertently produces the gene with the desired mutation. This vector is then introduced into a host cell and cloned.

Kits for SSM are based on PCR amplification and ligation to create a modified single strand of DNA. SSM kits differ in the type of mutation they induce, differentiating between insertion, deletions or base changes.

Kits express the efficiency of the mutation process through the percentage mutated sequences of the total final product. High-fidelity polymerases are specially engineered for increased accuracy, and thus will accurately copy your fragment, and in turn, increase the efficiency of mutations.

DNA Methylation

When methyl groups are added to a DNA sequence, it essentially ‘turns off’ a gene. Methylation modifies cytosine bases with the addition of a methyl group, which causes the repression of gene transcription. In nature, DNA Methylation is utilized for cellular differentiation and development. In the lab, it can be used to identify the function of a gene and its downstream effects.

The efficiency of this technique is related to the methods used to select the mutated sequence over the original template. The most common techniques are selection against DNA methylation, a signature of “naturally” amplified DNA, or by base uracil substitution in the template strand that will result in the destruction of the template itself. Bisulfite conversion is the most popular method; converting unmethylated cytosines to uracil, therefore enabling identification of methylation patterns.

Transposon mutagenesis

Transposons are sequences of DNA which are able to move around the genome and insert themselves at certain positions. This method makes use of the enzyme transposase, which binds to the end of transposable DNA elements, (so-called transposons); catalyzing their movement in the genome by a cut and paste method. This method often results in duplication events and may thus alter the cell’s genome significantly. Transposons can also be inserted into a plasmid. This technique can mutate an existing gene and subsequently silence it by insertion within the gene. Alternatively, it can activate a gene by inserting upstream, where its promoter initiates transcription of the transposon and the target gene.

For further guidance see our Gene Modification Troubleshoot.

Compare Sited-Directed Mutagenesis Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison engine does not show the best kit, but instead helps you, the researcher, choose the most suitable kit
for your experiment.

Go to our How it Works page for a guide to using the ZAGENO comparison engine.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video Credits

Video: Garvan Institute of Medical Research/YouTube