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Gene Modification Kits

Score 2.50

EZ-96 DNA Methylation-Lightning™ Kit

Zymo Research

Control Type
/
/
Sample Amount
100 pg - 2 µg DNA
100 pg - 2 µg DNA
Cloning Time
/
/
From
$ 442.00 (192 Reactions)
Sizes
2 (192 Reactions)
Catalog IDs
D5032, D5033
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Gene Modification Kits

Score 6.60

Cells-to-CpG™ Bisulfite Conversion Kit

Applied Biosystems

Control Type
/
/
Sample Amount
0.1-1 µg purified gDNA, 5000-10^5 cells, 2.5 µl blood
0.1-1 µg purified gDNA, 5000-10^5 cells, 2.5 µl blood
Cloning Time
/
/
From
$ 400.00 (50 Reactions)
Sizes
2 (50 - 192 Reactions)
Catalog IDs
4445555, 4445554
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Gene Modification Kits

Score 10.00

EZ DNA Methylation™ Kit

Zymo Research

Control Type
/
/
Sample Amount
500 pg - 2 µg DNA
500 pg - 2 µg DNA
Cloning Time
/
/
From
$ 138.00 (50 Reactions)
Sizes
2 (50 - 200 Reactions)
Catalog IDs
D5001, D5002
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Gene Modification Kits

Score 10.00

Transformer™ Site-Directed Mutagenesis Kit

Clontech

Control Type
Negative, Positive
Negative, Positive
Sample Amount
2.0 µl
2.0 µl
Cloning Time
/
/
From
$ 473.00 (30 Reactions)
Sizes
1 (30 Reactions)
Catalog IDs
630702
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Gene Modification Kits

Score 10.00

Diversify™ PCR Random Mutagenesis Kit

Clontech

Control Type
Positive
Positive
Sample Amount
~1 ng/µl
~1 ng/µl
Cloning Time
/
/
From
$ 640.00 (30 Reactions)
Sizes
1 (30 Reactions)
Catalog IDs
630703
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Gene Modification Kits

Score 0.80

QuikChange Lightning Multi Site-Directed Mutagenesis Kit, 30 rnx (Academic)

Agilent

Control Type
/
/
Sample Amount
/
/
Cloning Time
/
/
From
$ 377.00 (10 Reactions)
Sizes
2 (10 - 30 Reactions)
Catalog IDs
210515, 210513
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Gene Modification Kits

QuikChange Multi Site-Directed Mutagenesis Kit, 30 rxn (Academic)

Agilent

Control Type
/
/
Sample Amount
/
/
Cloning Time
/
/
From
$ 402.00 (10 Reactions)
Sizes
2 (10 - 30 Reactions)
Catalog IDs
200515, 200514
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Gene Modification Kits

Score 5.00

EZ RNA Methylation™ Kit

Zymo Research

Control Type
/
/
Sample Amount
32-3000 ng
32-3000 ng
Cloning Time
/
/
From
$ 189.00 (50 Preparations)
Sizes
2 (50 - 200 Preparations)
Catalog IDs
R5001, R5002
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Gene Modification Kits

EZ-96 DNA Methylation™ MagPrep

Zymo Research

Control Type
/
/
Sample Amount
500 pg - 2µg DNA
500 pg - 2µg DNA
Cloning Time
/
/
From
$ 601.00 (384 Reactions)
Sizes
2 (384 - 764 Reactions)
Catalog IDs
D5040, D5041
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Gene Modification Kits

Score 10.00

EZ DNA Methylation-Direct™ Kit

Zymo Research

Control Type
/
/
Sample Amount
1 x 10^3 - 8 x 10^4 cells, 50 pg - 2 µg DNA
1 x 10^3 - 8 x 10^4 cells, 50 pg - 2 µg DNA
Cloning Time
/
/
From
$ 193.00 (50 Reactions)
Sizes
2 (50 - 200 Reactions)
Catalog IDs
D5020, D5021
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Gene Modification Kits

Score 8.80

Cells-to-CpG™ Bisulfite Conversion & Quantitation Control Kit

Applied Biosystems

Control Type
/
/
Sample Amount
0.1-1 µg purified gDNA, 500-10^5 cells, 2.5 µl Blood
0.1-1 µg purified gDNA, 500-10^5 cells, 2.5 µl Blood
Cloning Time
/
/
From
$ 800.00 (192 Reactions)
Sizes
1 (192 Reactions)
Catalog IDs
4445553
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Gene Modification Kits

EZ-96 DNA Methylation-Direct™ MagPrep

Zymo Research

Control Type
/
/
Sample Amount
1 x 10^3 - 8 x 10^4 cells, 50 pg - 2 μg of DNA
1 x 10^3 - 8 x 10^4 cells, 50 pg - 2 μg of DNA
Cloning Time
/
/
From
$ 696.00 (384 Reactions)
Sizes
2 (384 - 768 Reactions)
Catalog IDs
D5044, D5045
Added to comparison remove item

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Gene Modification Application

Gene Modification refers to numerous gene editing techniques that can introduce mutations into double stranded DNA. Whilst the CRISPR-Cas9 system induces site-specific mutation in vivo, other genetic engineering techniques can introduce such changes during your sub-cloning steps or in vitro. Such gene modification techniques can also be used to change the specificity of RNA interference approaches.

Site Specific Mutagenesis

Site-specific mutagenesis (SSM) inserts, deletes, or substitutes a single base within the DNA sequence of a plasmid. Doing so allows for the investigation of the biological activity of selected genes and proteins.

SSM procedure starts with the synthesis of a short DNA primer, which contains your desired mutation. This synthetic primer should also be complementary to its DNA template strand so that it can hybridize with the gene of interest. Next, this primer is extended via a DNA polymerase, which inadvertently produces the gene with the desired mutation. This vector is then introduced into a host cell and cloned.

Kits for SSM are based on PCR amplification and ligation to create a modified single strand of DNA. SSM kits differ in the type of mutation they induce, differentiating between insertion, deletions or base changes.

Kits express the efficiency of the mutation process through the percentage mutated sequences of the total final product. High-fidelity polymerases are specially engineered for increased accuracy, and thus will accurately copy your fragment, and in turn, increase the efficiency of mutations.

DNA Methylation

When methyl groups are added to a DNA sequence, it essentially ‘turns off’ a gene. Methylation modifies cytosine bases with the addition of a methyl group, which causes the repression of gene transcription. In nature, DNA Methylation is utilized for cellular differentiation and development. In the lab, it can be used to identify the function of a gene and its downstream effects.

The efficiency of this technique is related to the methods used to select the mutated sequence over the original template. The most common techniques are selection against DNA methylation, a signature of “naturally” amplified DNA, or by base uracil substitution in the template strand that will result in the destruction of the template itself. Bisulfite conversion is the most popular method; converting unmethylated cytosines to uracil, therefore enabling identification of methylation patterns.

Transposon mutagenesis

Transposons are sequences of DNA which are able to move around the genome and insert themselves at certain positions. This method makes use of the enzyme transposase, which binds to the end of transposable DNA elements, (so-called transposons); catalyzing their movement in the genome by a cut and paste method. This method often results in duplication events and may thus alter the cell’s genome significantly. Transposons can also be inserted into a plasmid. This technique can mutate an existing gene and subsequently silence it by insertion within the gene. Alternatively, it can activate a gene by inserting upstream, where its promoter initiates transcription of the transposon and the target gene.

For further guidance see our Gene Modification Troubleshoot.

Compare Sited-Directed Mutagenesis Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison engine does not show the best kit, but instead helps you, the researcher, choose the most suitable kit
for your experiment.

Go to our How it Works page for a guide to using the ZAGENO comparison engine.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video Credits

Video: Garvan Institute of Medical Research/YouTube