Gene Silencing Functionality
Gene Silencing refers to a series of gene editing techniques that reduces the transcription or translation of a gene. In contrast with genetic mutations, gene silencing selectively targets the gene of interest with various silencing intensities, taking advantage of the native gene regulation.
Recently, gene silencing was recognised to be crucial in the study of autophagy.
RNA interference (RNAi)
RNA interference (RNAi) is a widely used gene silencing technique for the regulation of gene expression. Compared to classic mutants, gene silencing techniques can be used with an inducible promoter or under a tissue- or stage-specific control. When choosing an RNAi kit, there are a few factors to consider.
Firstly, whether you use a synthesized siRNA or if you'd prefer to clone your own. The former is a faster, high-quality solution, whereas custom made synthetic siRNA is expensive. On the other hand, in vivo creation of siRNA creates an entire collection of siRNA against a target gene, which increases the chances of a successful silencing. While this is a lengthy process, there are kits that can make the process easier.
Secondly, the method of siRNA delivery in the cell. While synthesized siRNAs can be delivered directly with dedicated reagents or physical methods such as magnetic beads, it is also possible to express RNAs that will be processed in the cell to become siRNAs. This approach requires the use of specific vectors, but is advantageous by using selectable markers or time- and cell-type- specific expression.
Use our Gene Silencing Troubleshoot to fix any problems which arise.
Compare Gene Silencing Kits
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