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Gene Transfer Reagents

CANFAST™ Transfection Reagent

Canvax Biotech

From
$ 199.00 (1.5 ml)
Sizes
1 (1.5 ml)
Catalog IDs
T0082
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Gene Transfer Reagents

LightSwitch™ Transfection Optimization Kit

Active Motif

Type of Transfer
/
/
Targets
/
/
Expression
/
/
From
$ 510.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
TFXOPT
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Gene Transfer Reagents

Human Stem Cell Nucleofector® Kit 1

Lonza

Type of Transfer
/
/
Targets
/
/
Expression
/
/
From
$ 399.00 (25 Reactions)
Sizes
1 (25 Reactions)
Catalog IDs
VPH-5012
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Gene Transfer Reagents

GFP Purified Protein

Applied Biological Materials

From
$ 75.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
000033P
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Gene Transfer Reagents

Score 0.80

RNAifectin™ Transfection Reagent

Applied Biological Materials

Type of Transfer
/
/
Targets
RNAi oligos
RNAi oligos
Expression
/
/
From
$ 165.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G073
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Gene Transfer Reagents

DNAfectin™ Plus Transfection Reagent

Applied Biological Materials

Type of Transfer
/
/
Targets
Genomic DNA
Genomic DNA
Expression
/
/
From
$ 195.00 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
G2500
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Gene Transfer Reagents

Score 0.80

DNAfectin™ 2100 Transfection Reagent

Applied Biological Materials

Type of Transfer
/
/
Targets
Genomic DNA
Genomic DNA
Expression
/
/
From
$ 145.00 (500 Reactions)
Sizes
1 (500 Reactions)
Catalog IDs
G2100
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Gene Transfer Reagents

Score 10.00

TurboFectin 8.0

Origene

Type of Transfer
/
/
Targets
/
/
Expression
/
/
From
$ 420.00 (1 Vial)
Sizes
2 (1 - 5 Vial)
Catalog IDs
TF81001, TF81005
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Gene Transfer Reagents

NanoFect Transfection Reagent

Alstem

Type of Transfer
/
/
Targets
siRNA
siRNA
Expression
/
/
From
$ 199.00 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
NF100
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Gene Transfer Reagents

LentiBlast™

OZ Biosciences

Type of Transfer
/
/
Targets
Viral RNA
Viral RNA
Expression
/
/
From
$ 164.85 (1000 µl)
Sizes
2 (1000 - 3000 µl)
Catalog IDs
LB00500, LB01500
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Gene Transfer Reagents

AdenoMag Transduction Reagent

OZ Biosciences

Type of Transfer
/
/
Targets
Viral DNA
Viral DNA
Expression
/
/
From
$ 129.00 (100 µl)
Sizes
4 (1 - 200 ml)
Catalog IDs
AM71000, KC30900, ...
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Gene Transfer Reagents

LipoMag Transfection Kit

OZ Biosciences

Type of Transfer
/
/
Targets
Genomic DNA, Plasmid DNA, mRNA, shRNA, siRNA
Genomic DNA, Plasmid DNA, mRNA, shRNA, siRNA
Expression
/
/
From
$ 321.85 (1 Kit)
Sizes
3 (1 - 2 Kit)
Catalog IDs
LM80000, LM80250, ...
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Gene Transfer Function & Application

Gene Transfer, also known as horizontal gene transfer, is the process of inserting a specific coding sequence into a cell, either as a plasmid or as a genomic insert. This Gene Editing technique aims to induce the synthesis of the target protein in the cell for later analysis or, for example, for therapeutic purposes.

For example, our articles about Controlling Cellular Growth with Light and Three-Parent Babies illustrate how gene transfer can be used to perform critical research.

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Transformation

Transformation - if the target is a non-animal cell.

Bacteria transformation is a staple technique in cell biology labs, used for cloning or protein expression. Transformation protocols rely on transforming bacterial cells into competent cells via chemical plasmid transformation or electroporation, with dedicated kits for both.

While chemical transformation is very cost-effective, electroporation can reach have high transformation efficiency. Keep in mind that electroporators can be used for other cells, such as plant cells, which might justify the investments.

Solve any experiement problems with our Transformation Troubleshoot.

Transfection & Transduction

Transfection - when a non-viral transfer method is used for genetic transfer.

Transduction - when a viral vector is used for genetic transfer.

The term “transfection” is mostly used for describing the insertion of nucleic acids into a cell by non-viral methods. The process is often carried out by electroporation, cell squeezing, or via chemical methods like calcium phosphate exposure that opens transient holes in cell membranes allowing target nucleic acids to enter into the cells. Sometimes liposomes are used to allow the membrane to be crossed without any permanent damage to the cell.

Perfect your experiement with our Transfection Troubleshoot.

For mammalian cells, the main factor to consider is cell type. There are different kits on the market optimizing the transfection or transduction process for specific cells types. Also, keep in mind that different kits can result in different expression levels. This is particularly important if your experimental set-up includes a phenotypical analysis with a range of expression levels of your protein, or if you expect a cytotoxic effect.

Master the technqiue with our Lentiviral Transduction Troubleshoot.

Moreover, if you want to transfer miRNA or siRNA instead of DNA, be sure to use a kit that specifically does that. In particular, it is possible to transduce purified siRNA with physical methods, without the use of vectors.