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Brand
101 Bio
Sizes
2 (100 - 1000 µl)
Available From
1 Vendor
Price Range
$ 109.00 - $ 269.00
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Brand
Mirus
Sizes
5 (300 - 10000 µl)
Available From
1 Vendor
Price Range
$ 128.00 - $ 4,069.00
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Brand
MilliporeSigma
Sizes
1 (1000 µl)
Available From
1 Vendor
Price
$ 223.76
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Brand
IBL-America
Sizes
1 (200 µl)
Available From
1 Vendor
Price
$ 105.60
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ViraDuctin™ Lentivirus Transduction Kit, Cell Biolabs
Type of Transfer
/
Targets
/
Expression
/
Brand
Cell Biolabs
Sizes
2 (40 - 200 Reactions)
Available From
1 Vendor
Price Range
$ 255.00 - $ 744.62
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ViraDuctin™ Retrovirus Transduction Kit, Cell Biolabs
Type of Transfer
/
Targets
/
Expression
/
Brand
Cell Biolabs
Sizes
2 (40 - 200 Reactions)
Available From
1 Vendor
Price Range
$ 332.31 - $ 775.38
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Brand
Bulldog Bio
Sizes
3 (500 - 5000 µl)
Available From
1 Vendor
Price Range
$ 200.87 - $ 1,437.05
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Transfection Reagent (in vitro) SKU: T8198-03
Type of Transfer
/
Targets
/
Expression
/
Brand
US Biological
Sizes
1 (1000 µl)
Available From
1 Vendor
Price
$ 533.00
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Brand
SignaGen Laboratories
Sizes
1 (1000 µl)
Available From
1 Vendor
Price
$ 169.00
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RetroNectin® GMP grade
Type of Transfer
/
Targets
/
Expression
/
Brand
TaKaRa
Sizes
1 (2500 µl)
Available From
1 Vendor
Price
$ 1,442.00
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pLenti-SFFV-VEGF165
Type of Transfer
/
Targets
/
Expression
/
Scientific Score
6.16
Brand
Applied Biological Materials
Sizes
1 (1 µg)
Available From
1 Vendor
Price
$ 355.00
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Penefect Transfection Reagent
Type of Transfer
/
Targets
/
Expression
/
Scientific Score
4.37
Brand
LifeSct
Sizes
2 (1000 - 5000 µl)
Available From
1 Vendor
Price Range
$ 49.00 - $ 199.00
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Gene Transfer Function & Application

Gene Transfer, also known as horizontal gene transfer, is the process of inserting a specific coding sequence into a cell, either as a plasmid or as a genomic insert. This Gene Editing technique aims to induce the synthesis of the target protein in the cell for later analysis or, for example, for therapeutic purposes.

For example, our articles about Controlling Cellular Growth with Light and Three-Parent Babies illustrate how gene transfer can be used to perform critical research.

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Transformation

Transformation - if the target is a non-animal cell.

Bacteria transformation is a staple technique in cell biology labs, used for cloning or protein expression. Transformation protocols rely on transforming bacterial cells into competent cells via chemical plasmid transformation or electroporation, with dedicated kits for both.

While chemical transformation is very cost-effective, electroporation can reach have high transformation efficiency. Keep in mind that electroporators can be used for other cells, such as plant cells, which might justify the investments.

Solve any experiement problems with our Transformation Troubleshoot.

Transfection & Transduction

Transfection - when a non-viral transfer method is used for genetic transfer.

Transduction - when a viral vector is used for genetic transfer.

The term “transfection” is mostly used for describing the insertion of nucleic acids into a cell by non-viral methods. The process is often carried out by electroporation, cell squeezing, or via chemical methods like calcium phosphate exposure that opens transient holes in cell membranes allowing target nucleic acids to enter into the cells. Sometimes liposomes are used to allow the membrane to be crossed without any permanent damage to the cell.

Perfect your experiement with our Transfection Troubleshoot.

For mammalian cells, the main factor to consider is cell type. There are different kits on the market optimizing the transfection or transduction process for specific cells types. Also, keep in mind that different kits can result in different expression levels. This is particularly important if your experimental set-up includes a phenotypical analysis with a range of expression levels of your protein, or if you expect a cytotoxic effect.

Master the technqiue with our Lentiviral Transduction Troubleshoot.

Moreover, if you want to transfer miRNA or siRNA instead of DNA, be sure to use a kit that specifically does that. In particular, it is possible to transduce purified siRNA with physical methods, without the use of vectors.