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Gene Transfer Reagents
CANFAST™ Transfection Reagent
Scientific Score
2.60
Brand
Canvax Biotech
Sizes
1 (1.5 ml)
Available From
1 Vendor
Price
$ 199.00
Catalog IDs
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Gene Transfer Reagents
K2® Transfection System
Type of Transfer
/
/
Targets
/
/
Expression
/
/
Scientific Score
6.92
Brand
Biontex
Sizes
4 (0.75 - 5 ml)
Available From
1 Vendor
Price Range
$ 170.00 - $ 1,276.00
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Gene Transfer Reagents
LipoD293™ In Vitro DNA Transfection Reagent
Brand
SignaGen Laboratories
Sizes
1 (1 ml)
Available From
1 Vendor
Price
$ 169.00
Catalog IDs
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Gene Transfer Reagents
pLenti-SFFV-VEGF165
Type of Transfer
/
/
Targets
/
/
Expression
/
/
Scientific Score
6.16
Brand
Applied Biological Materials
Sizes
1 (1 μg)
Available From
1 Vendor
Price
$ 355.00
Catalog IDs
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Gene Transfer Reagents
EZ-Tn5 <KAN-2> Insertion Kit
Scientific Score
0.72
Brand
Lucigen
Sizes
1 (10 Reactions)
Available From
1 Vendor
Price
$ 515.00
Catalog IDs
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Gene Transfer Reagents
Xfect Mouse Embryonic Stem Cell Transfection Reagent
Type of Transfer
/
/
Targets
Plasmid DNA
Plasmid DNA
Expression
/
/
Scientific Score
8.73
Brand
TaKaRa
Sizes
2 (100 - 300 Reactions)
Available From
1 Vendor
Price
$ 238.00
Catalog IDs
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Gene Transfer Reagents
CalPhos™ Mammalian Transfection Kit
Type of Transfer
/
/
Targets
Plasmid DNA
Plasmid DNA
Expression
/
/
Scientific Score
8.80
Brand
TaKaRa
Sizes
1 (700 Reactions)
Available From
1 Vendor
Price
$ 239.00
Catalog IDs
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Gene Transfer Reagents
TurboFect in vivo Transfection Reagent
Type of Transfer
/
/
Targets
Plasmid DNA
Plasmid DNA
Expression
/
/
Scientific Score
6.64
Brand
GE Healthcare Life Sciences
Sizes
1 (120 µl)
Available From
1 Vendor
Price
$ 420.00
Catalog IDs
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Gene Transfer Reagents
LentiBlast™
Type of Transfer
/
/
Targets
Viral RNA
Viral RNA
Expression
/
/
Scientific Score
9.28
Brand
OZ Biosciences
Sizes
2 (1000 - 3000 µl)
Available From
2 Vendors
Price Range
$ 164.85 - $ 369.23
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Gene Transfer Reagents
AdenoMag Transduction Reagent
Type of Transfer
/
/
Targets
Viral DNA
Viral DNA
Expression
/
/
Scientific Score
9.38
Brand
OZ Biosciences
Sizes
4 (1 - 200 ml)
Available From
2 Vendors
Price Range
$ 129.00 - $ 981.59
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Gene Transfer Reagents
LipoMag Transfection Kit
Type of Transfer
/
/
Targets
Genomic DNA, Plasmid DNA, mRNA, shRNA, siRNA
Genomic DNA, Plasmid DNA, mRNA, shRNA, siRNA
Expression
/
/
Scientific Score
9.04
Brand
OZ Biosciences
Sizes
3 (1 - 2 Kit)
Available From
2 Vendors
Price Range
$ 321.85 - $ 775.59
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Gene Transfer Reagents
In vivo DogtorMag
Type of Transfer
/
/
Targets
Plasmid DNA, mRNA, shRNA, siRNA
Plasmid DNA, mRNA, shRNA, siRNA
Expression
/
/
Scientific Score
8.77
Brand
OZ Biosciences
Sizes
4 (1 - 500 ml)
Available From
2 Vendors
Price Range
$ 145.55 - $ 728.21
Added to comparison remove item

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Gene Transfer Function & Application

Gene Transfer, also known as horizontal gene transfer, is the process of inserting a specific coding sequence into a cell, either as a plasmid or as a genomic insert. This Gene Editing technique aims to induce the synthesis of the target protein in the cell for later analysis or, for example, for therapeutic purposes.

For example, our articles about Controlling Cellular Growth with Light and Three-Parent Babies illustrate how gene transfer can be used to perform critical research.

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Transformation

Transformation - if the target is a non-animal cell.

Bacteria transformation is a staple technique in cell biology labs, used for cloning or protein expression. Transformation protocols rely on transforming bacterial cells into competent cells via chemical plasmid transformation or electroporation, with dedicated kits for both.

While chemical transformation is very cost-effective, electroporation can reach have high transformation efficiency. Keep in mind that electroporators can be used for other cells, such as plant cells, which might justify the investments.

Solve any experiement problems with our Transformation Troubleshoot.

Transfection & Transduction

Transfection - when a non-viral transfer method is used for genetic transfer.

Transduction - when a viral vector is used for genetic transfer.

The term “transfection” is mostly used for describing the insertion of nucleic acids into a cell by non-viral methods. The process is often carried out by electroporation, cell squeezing, or via chemical methods like calcium phosphate exposure that opens transient holes in cell membranes allowing target nucleic acids to enter into the cells. Sometimes liposomes are used to allow the membrane to be crossed without any permanent damage to the cell.

Perfect your experiement with our Transfection Troubleshoot.

For mammalian cells, the main factor to consider is cell type. There are different kits on the market optimizing the transfection or transduction process for specific cells types. Also, keep in mind that different kits can result in different expression levels. This is particularly important if your experimental set-up includes a phenotypical analysis with a range of expression levels of your protein, or if you expect a cytotoxic effect.

Master the technqiue with our Lentiviral Transduction Troubleshoot.

Moreover, if you want to transfer miRNA or siRNA instead of DNA, be sure to use a kit that specifically does that. In particular, it is possible to transduce purified siRNA with physical methods, without the use of vectors.