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Gene Transfer Reagents

Score 10.00

Lipofectamine® RNAiMAX Transfection Reagent

Invitrogen

Control Type
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/
Sample Amount
/
/
Quantity
0.1 ml, 0.3 ml, 0.75 ml, 1.5 ml, 15 ml
0.1 ml, 0.3 ml, 0.75 ml, 1.5 ml, 15 ml
From
$ 77.00 (100 µl)
Sizes
5 (1.5 - 15000 ml)
Catalog IDs
13778150, 13778100, ...
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Gene Transfer Reagents

Score 0.80

Xfect Mouse Embryonic Stem Cell Transfection Reagent

Clontech

Control Type
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/
Sample Amount
5 x 10^5 –1 x 10^6 cells, 2.5 -7.5 µg Plasmid DNA
5 x 10^5 –1 x 10^6 cells, 2.5 -7.5 µg Plasmid DNA
Quantity
/
/
From
$ 238.00 (100 Reactions)
Sizes
2 (100 - 300 Reactions)
Catalog IDs
631320, 631321
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Gene Transfer Reagents

Score 10.00

CalPhos™ Mammalian Transfection Kit

Clontech

Control Type
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Sample Amount
2-4 x 10^5 cells/well, 0.03-27 µg Plasmid DNA
2-4 x 10^5 cells/well, 0.03-27 µg Plasmid DNA
Quantity
/
/
From
$ 239.00 (700 Reactions)
Sizes
1 (700 Reactions)
Catalog IDs
631312
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Gene Transfer Reagents

TurboFect in vivo Transfection Reagent

GE Healthcare Life Sciences

Control Type
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Sample Amount
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Quantity
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From
$ 420.00 (120 µl)
Sizes
1 (120 µl)
Catalog IDs
R0541
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Gene Transfer Reagents

LentiBlast™

OZ Biosciences

Control Type
/
/
Sample Amount
3 x 10^3 - 2 x 10^5 cells
3 x 10^3 - 2 x 10^5 cells
Quantity
/
/
From
$ 164.85 (1000 µl)
Sizes
2 (1000 - 3000 µl)
Catalog IDs
LB00500, LB01500
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Gene Transfer Reagents

AdenoMag Transduction Reagent

OZ Biosciences

Control Type
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Sample Amount
1 x 10^4 - 5 x 10^5 cells
1 x 10^4 - 5 x 10^5 cells
Quantity
/
/
From
$ 129.00 (100 µl)
Sizes
4 (1 - 200 ml)
Catalog IDs
AM71000, KC30900, ...
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Gene Transfer Reagents

LipoMag Transfection Kit

OZ Biosciences

Control Type
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Sample Amount
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Quantity
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From
$ 321.85 (1 Kit)
Sizes
3 (1 - 2 Kit)
Catalog IDs
LM80000, LM80250, ...
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Gene Transfer Reagents

In vivo DogtorMag

OZ Biosciences

Control Type
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Sample Amount
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Quantity
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From
$ 145.55 (1 Kit)
Sizes
4 (1 - 500 ml)
Catalog IDs
IV-DM31000, IV-KC30220, ...
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Gene Transfer Reagents

Ab-DeliverIN Transfection Reagent + FITC

OZ Biosciences

Control Type
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Sample Amount
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Quantity
/
/
From
$ 180.40 (100 µl)
Sizes
4 (1 - 250 ml)
Catalog IDs
AI21000, AI20500, ...
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Gene Transfer Reagents

ViroMag CRISPR

OZ Biosciences

Control Type
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Sample Amount
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Quantity
/
/
From
$ 232.78 (200 µl)
Sizes
3 (1 - 1000 Kit)
Catalog IDs
KVC50100, VMC50200, ...
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Gene Transfer Reagents

Score 0.80

RNAifectin™ Transfection Reagent

Applied Biological Materials

Control Type
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Sample Amount
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Quantity
1.0 ml
1.0 ml
From
$ 165.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G073
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Gene Transfer Reagents

DNAfectin™ Plus Transfection Reagent

Applied Biological Materials

Control Type
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/
Sample Amount
/
/
Quantity
1.0 ml
1.0 ml
From
$ 195.00 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
G2500
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Gene Transfer Function & Application

Gene Transfer, also known as horizontal gene transfer, is the process of inserting a specific coding sequence into a cell, either as a plasmid or as a genomic insert. This Gene Editing technique aims to induce the synthesis of the target protein in the cell for later analysis or, for example, for therapeutic purposes.

For example, our articles about Controlling Cellular Growth with Light and Three-Parent Babies illustrate how gene transfer can be used to perform critical research.

Transformation

Transformation - if the target is a non-animal cell.

Bacteria transformation is a staple technique in cell biology labs, used for cloning or protein expression. Transformation protocols rely on transforming bacterial cells into competent cells via chemical plasmid transformation or electroporation, with dedicated kits for both.

While chemical transformation is very cost-effective, electroporation can reach have high transformation efficiency. Keep in mind that electroporators can be used for other cells, such as plant cells, which might justify the investments.

Solve any experiement problems with our Transformation Troubleshoot.

Transfection & Transduction

Transfection - when a non-viral transfer method is used for genetic transfer.

Transduction - when a viral vector is used for genetic transfer.

The term “transfection” is mostly used for describing the insertion of nucleic acids into a cell by non-viral methods. The process is often carried out by electroporation, cell squeezing, or via chemical methods like calcium phosphate exposure that opens transient holes in cell membranes allowing target nucleic acids to enter into the cells. Sometimes liposomes are used to allow the membrane to be crossed without any permanent damage to the cell.

Perfect your experiement with our Transfection Troubleshoot.

For mammalian cells, the main factor to consider is cell type. There are different kits on the market optimizing the transfection or transduction process for specific cells types. Also, keep in mind that different kits can result in different expression levels. This is particularly important if your experimental set-up includes a phenotypical analysis with a range of expression levels of your protein, or if you expect a cytotoxic effect.

Master the technqiue with our Lentiviral Transduction Troubleshoot.

Moreover, if you want to transfer miRNA or siRNA instead of DNA, be sure to use a kit that specifically does that. In particular, it is possible to transduce purified siRNA with physical methods, without the use of vectors.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.