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In Vitro Transcription & Translation on ZAGENO

HiScribe™ T7 High Yield RNA Synthesis Kit New England Biolabs
From $ 217.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs E2040S
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MAXIscript® T7/T3 Kit (50 Reactions each) with Manual Invitrogen
From $ 322.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs AM1326M
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HiScribe™ T7 ARCA mRNA Kit New England Biolabs
From $ 307.00 (20 Reactions)
Sizes 1 (20 Reactions)
Catalog IDs E2065S
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T7 Transcription Kit Jena Bioscience
From $ 74.00 (40 Reactions)
Sizes 2 (40 - 200 Reactions)
Catalog IDs PCR-601S, PCR-601L
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T7 High Yield In Vitro Transcription Kit MCLAB
From $ 110.00 (20 Reactions)
Sizes 2 (20 - 100 Reactions)
Catalog IDs T7HT-100, T7HT-200
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MEGAscript® T3 Kit Invitrogen
From $ 319.00 (40 Reactions)
Sizes 1 (40 Reactions)
Catalog IDs AM1338
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AmpliCap-Max™ T7 High Yield Message Maker Kit CellScript
From $ 328.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs C-ACM04037
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EnGen® sgRNA Synthesis Kit, S. pyogenes New England Biolabs
From $ 400.00 (20 Reactions)
Sizes 1 (20 Reactions)
Catalog IDs E3322S
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MEGAscript® T7 Transcription Kit Invitrogen
From $ 217.00 (25 Reactions)
Sizes 3 (25 - 200 Reactions)
Catalog IDs AM1333, AM1334, ...
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MAXIscript® T3 Transcription Kit Invitrogen
From $ 170.00 (30 Reactions)
Sizes 1 (30 Reactions)
Catalog IDs AM1316
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HiScribe™ T7 Quick High Yield RNA Synthesis Kit New England Biolabs
From $ 266.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs E2050S
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Poly(A) Tailing Kit Ambion
From $ 210.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs AM1350
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The in vitro transcription process

The starting template of in vitro transcription is a linear DNA template that includes a promoter region together with the usual ingredients for a successful transcription process: RNA polymerase, nucleotides, and an appropriate buffer system. Since every reaction is different, the conditions may vary depending on your DNA template, expected RNA yield, length, and purity.

Make use of our In Vitro Transcription Troubleshoot for any experimental issues.

Templates used

You need a double stranded DNA (dsDNA) template, but its origin is irrelevant. The template can be anything from normal PCR reactions, plasmids, or even cDNA templates that themselves were generated from RNA molecules. However, different kits may be suitable for different templates, so take care when choosing your kit.

  • In order to use a PCR product in an in vitro transcription reaction, you have to add a promoter to your fragment so that it is recognized by RNA polymerase. The promoter is added at the 5’ end of the fragment.
  • Plasmid vectors must first be linearized before the transcription reaction can ensue. This is (fairly) easily done by using restriction enzymes. Purification should be carried out after such a digest as the restriction enzymes may inhibit the downstream in vitro transcription process.
  • One of the latest developments in this field is using in vitro transcription in RNA amplification reactions. Here, the reaction template is created from RNA during reverse transcription, generating cDNA. A second strand synthesis reaction is then performed to convert the cDNA to dsDNA for the in vitro transcription to be carried out.

Other applications also exists such as the use of oligonucleotides as templates.

Compare In Vitro Transcription Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.