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DNA & RNA Extraction on Zageno

QuickExtract™ RNA Extraction Kit Epicentre
Extraction of Total RNA Sample Type Cells, Culture Time to Sample ~ 30 min (with DNase I Treatment)
From $ 500.00 (500 Preparations)
Sizes 1 (500 Preparations)
Catalog IDs QER090150
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Food Oil DNA Isolation Kit Norgen Biotek
Extraction of Genomic DNA Sample Type Food Time to Sample 25 min / 10 preparations
From $ 201.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 61700
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XIT™ Genomic DNA from FFPE Tissue G-Biosciences
Extraction of Genomic DNA Sample Type FFPE tissue Time to Sample 300 min
From $ 101.00 (25 Preparations)
Sizes 1 (25 Preparations)
Catalog IDs 786-290
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ZR Plant/Seed DNA MiniPrep™ Zymo Research
Extraction of Genomic DNA, Viral DNA, Mitochondrial DNA Sample Type Seeds, Tissue Time to Sample 15 min
From $ 216.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6020
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NucleoSpin® 8 Tissue Core Kit Macherey-Nagel
Extraction of Genomic DNA Sample Type Cells, Culture, Tissue Time to Sample 20 min / 6 strips (after lysis)
From $ 1,058.00 (384 Preparations)
Sizes 1 (384 Preparations)
Catalog IDs 740453.4
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NucleoSpin® RNA Blood Macherey-Nagel
Extraction of Total RNA Sample Type Blood Time to Sample 55 min / 6 preparations
From $ 57.00 (10 Preparations)
Sizes 2 (10 - 50 Preparations)
Catalog IDs 740200.10, 740200.50
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Direct DNA Extraction Kit (Bacteria) Norgen Biotek
Extraction of Bacterial DNA Sample Type Culture, Food Time to Sample 20 min
From $ 102.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 61500
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Urine Cell-Free Circulating RNA Purification Mini Kit Norgen Biotek
Extraction of miRNA, Circulating RNA, Exosomal RNA Sample Type Urine Time to Sample 25-30 min / 10 preparations
From $ 345.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 56900
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Urine Cell-Free Circulating DNA Purification Midi Kit Norgen Biotek
Extraction of Viral DNA, Circulating DNA Sample Type Urine Time to Sample 40-45 min / 10 preparations
From $ 350.00 (20 Preparations)
Sizes 1 (20 Preparations)
Catalog IDs 56700
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illustra Nucleon HT GE Life Sciences
Extraction of Genomic DNA Sample Type FFPE tissue Time to Sample ~ 360 min
From $ 291.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs RPN8509
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GET™ Total RNA G-Biosciences
Extraction of Total RNA Sample Type Blood, Cells, Culture, Tissue Time to Sample < 60 min
From $ 260.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs 786-132
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QuickClean 96-well Plasmid Miniprep Kit GenScript
Extraction of Plasmid DNA Sample Type Culture Time to Sample 60 min
From $ 488.00 (384 Preparations)
Sizes 1 (384 Preparations)
Catalog IDs L00237
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

DNA Extraction

DNA Purification, which commonly uses magnetic beads or silica columns, requires these steps:

• Cell lysis via the use of:
* Chaotropic Salts – make it possible for DNA to bind to solid phase (e.g. silica membrane)
* Detergents – able to degrade proteins and cellular structures, including the nuclear envelope
* Enzymes – such as proteinase K and RNases, which break down proteins and RNA respectively

• Removal of contaminants (e.g. carbohydrates) through centrifugation and pipetting, specific methods include:
* Ethanol Precipitation – DNA is insoluble in alcohol
* Phenol-Chloroform Extraction – Phenol removes proteins in the aqueous layer
* Mini-column / Spin Column Purification – DNA will bind to silica under the correct conditions

Check out our article about the Coral Reefs and DNA extraction tips for working with this sample type.

RNA Extraction

RNA Purification, in general, runs as follows:

  • Guanidine thiocyanate and a reducing agent disrupt the cells
  • Vigorous shaking or vortexing break any disulfide bonds as well as inactivating any contaminants
  • Phenol and chloroform-isoamyl, followed by centrifugation, separate RNA in the cell
  • Ethanol washing (75%) removes impurities from the precipitate.

Compare RNA Extraction Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For instance, this comparison shows:

  • that the High Pure RNA Isolation kit from Roche Life Science is the cheapest per preperation
  • that the Qiaamp RNA Blood Mini Kit from Qiagen has the greatest binding capacity
  • that the Total RNA Purification Kit from Norgen Biotek can also be used to extract mRNA and miRNA

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for
your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Videos:

MrSimpleScience/YouTube

ChangBenson/YouTube