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Protein Purification on Zageno

DynaMag™-96 Side Skirted Magnet Invitrogen
Target Protein / Sample Amount / Preparation Method /
From Not available
Sizes 1 (1 Magnet)
Catalog IDs 12027
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ProteoMiner™ Protein Enrichment Introductory Large-Capacity Kit Bio-Rad
Target Protein Low abundance protein Sample Amount > 50 mg Preparation Method Enrichment
From $ 255.00 (2 Preparations)
Sizes 1 (2 Preparations)
Catalog IDs 1633009
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Qproteome FFPE Tissue Kit QIAGEN
Target Protein Total protein Sample Amount 2–3 x 100 mm in diameters Preparation Method Fractionation
From $ 404.00 (20 Preparations)
Sizes 1 (20 Preparations)
Catalog IDs 37623
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Ni-NTA Superflow 96 BioRobot Kit QIAGEN
Target Protein Tagged protein Sample Amount 600 µg Preparation Method Tag
From $ 1,393.00 (384 Preparations)
Sizes 1 (384 Preparations)
Catalog IDs 969261
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InGel™ Blue G-Biosciences
Target Protein Total protein Sample Amount 1-2 mm gel cubes Preparation Method Digest, Purification
From $ 181.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs 786-681
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InGel™ Silver G-Biosciences
Target Protein Total protein Sample Amount 1-2 mm gel cubes Preparation Method Digest, Purification
From $ 194.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs 786-241
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GST Fusion Protein Purification Kit GenScript
Target Protein Tagged protein Sample Amount / Preparation Method Purification
From $ 139.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs L00207
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PureCube Ni-NTA Agarose Cube Biotech
Target Protein Tagged protein Sample Amount / Preparation Method Tag
From $ 113.00 (10 ml)
Sizes 3 (10 - 250 ml)
Catalog IDs 31103, 31105, 31110
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PureCube NTA Agarose Cube Biotech
Target Protein Tagged protein Sample Amount / Preparation Method Tag
From $ 113.00 (10 ml)
Sizes 3 (10 - 250 ml)
Catalog IDs 31703, 31705, 31710
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PureCube Amine Activated Agarose Cube Biotech
Target Protein Other protein Sample Amount / Preparation Method Purification
From $ 114.00 (10 ml)
Sizes 3 (10 - 250 ml)
Catalog IDs 51003, 51005, 51010
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PureCube Maleimide Activated Agarose Cube Biotech
Target Protein Other protein Sample Amount / Preparation Method Purification
From $ 337.00 (10 ml)
Sizes 2 (10 - 50 ml)
Catalog IDs 51103, 51105
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PureCube Alprenolol Agarose Cube Biotech
Target Protein Other protein Sample Amount / Preparation Method Purification
From $ 780.00 (1 ml)
Sizes 2 (1 - 5 ml)
Catalog IDs 56031, 56032
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

His-tag and GST-tag

His-tag and GST-tag protein fusions are commonly used for heterologous protein isolation from E.coli BL21. However, when expressing a eukaryotic protein in prokaryotic systems, you might experience protein degradation and protein precipitation.In this case, or if you plan on examining post translational protein modifications such as protein phosphorylation, you should consider a eukaryotic expression system. While this results in protein modifications that are closer to those of native proteins, they might give some problems when used in combination with some purification tags.

Check our Protein Purification Troubleshoot for tips on perfecting your experiment.

Protein Size

Protein size is also something to consider. The relative size of your tag of choice compared to the protein might interfere with protein folding. For example, it is not advised to use Glutathione S-transferase (GST) fusion for the isolation of small protein for this reason. An alternative would be to use immunoaffinity chromatography, which does not require tagged proteins and can be used for natural sources.

Explore our article: Malaria - Humanity's Deadliest Predator, for more information on the research uses of Protein Enrichment Kits.

Buffer

The buffer used in extraction and purification might also interfere with the protein function or protein assays. High detergent concentration can interfere with protein folding which affects protein activity or protein-protein binding. This can be problematic when determining protein concentration, in particular using the Bradford method.

Compare Protein Purification Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For instance, this comparison shows:

  • that the Minute Plasma Membrane Protein Isolation Kit kit from Invent Biotechnologies is the cheapest per preparation
  • that the Readyprep Protein Extraction Kit from Bio-Rad accepts samples from the widest range of organsims.
  • that the Focus Subcell Kit from G-Biosciences can extract different types of protein.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video and image credits:

Video: David Johnson, PhD/YouTube