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Real-Time PCR Kits

Score 8.76

qMAXSen™ Green qPCR MasterMix (High ROX™)

Canvax Biotech

From
$ 99.00 (250 Reactions)
Sizes
4 (250 - 5000 Reactions)
Catalog IDs
E0532, E0539, ...
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Real-Time PCR Kits

Score 8.75

AMPLIFYME SYBR Universal Mix

BLIRT

From
$ 240.62 (200 Reactions)
Sizes
2 (200 - 2000 Reactions)
Catalog IDs
AM02-020, AM02-200
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Real-Time PCR Kits

Score 9.70

RealMOD™ Probe W² 2x qPCR mix

iNtRON

Multiplex PCR
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Reference Dye
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Templates
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From
$ 72.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
25351
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Real-Time PCR Kits

Score 8.69

KiloGreen 2X qPCR MasterMix-iCycler

Applied Biological Materials

From
$ 125.00 (500 Reactions)
Sizes
1 (500 Reactions)
Catalog IDs
MasterMix-KC
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Real-Time PCR Kits

Score 8.75

Fast EvaGreen Master Mix for qPCR(200 rxn)

Biotium

Multiplex PCR
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Reference Dye
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Templates
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From
$ 63.00 (1 ml)
Sizes
3 (1 - 5 ml)
Catalog IDs
31003-T, 31003, ...
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Real-Time PCR Kits

Score 9.71

Human Mesenchymal Stem Cell qPCR Array SKU: 07543

STEMCELL Technologies

Multiplex PCR
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Reference Dye
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Templates
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From
$ 330.00 (1 Plate)
Sizes
1 (1 Plate)
Catalog IDs
07543
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Real-Time PCR Kits

Score 9.71

Human Pluripotent Stem Cell-Derived Endoderm qPCR Array SKU: 07531

STEMCELL Technologies

Multiplex PCR
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Reference Dye
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Templates
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From
$ 330.00 (1 Plate)
Sizes
1 (1 Plate)
Catalog IDs
07531
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Real-Time PCR Kits

Score 9.71

Human Pluripotent Stem Cell-Derived Endoderm qPCR Array SKU: 07533

STEMCELL Technologies

Multiplex PCR
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Reference Dye
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Templates
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/
From
$ 330.00 (1 Plate)
Sizes
1 (1 Plate)
Catalog IDs
07533
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Real-Time PCR Kits

Score 8.69

TransScript® II Probe One-Step qRT-PCR SuperMix

TransGen Biotech

From
$ 196.92 (100 Reactions)
Sizes
2 (100 - 400 Reactions)
Catalog IDs
AQ321-01, AQ321-02
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Real-Time PCR Kits

Score 8.69

QPCR Kit, DNA, Giardia intestinalis

Cole-Parmer

From
$ 964.09 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
93957-52
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Real-Time PCR Kits

Score 9.71

ExonGreen qPCR Master Mix - Low Rox

Exon Biosciences

Multiplex PCR
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Reference Dye
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Templates
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From
$ 72.50 (100 Reactions)
Sizes
3 (100 - 1000 Reactions)
Catalog IDs
qpcrprobe100rxn1mL-2, qpcrprobe100rxn1mL-5, ...
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Real-Time PCR Kits

Score 9.72

ExonGreen Template-to-Ct OneStep qRT-PCR Master Mix - Low Rox

Exon Biosciences

Multiplex PCR
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Reference Dye
Low ROX
Low ROX
Templates
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/
From
$ 299.99 (100 Reactions)
Sizes
3 (100 - 1000 Reactions)
Catalog IDs
13AqrtpcrSYBR100rxn1mL-2, 13AqrtpcrSYBR100rxn1mL-5, ...
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qPCR Function

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR. With the added step of fluorescent labeling, it is possible to quantify the abundance of the amplified DNA using so-called quantitative real-time PCR (qPCR). When qPCR is used in combination with reverse transcription and cDNA synthesis, this is called RT-qPCR, a common technique for the analysis of gene expression.

Read about how qPCR has aided recent research: HIV 'Cure'? Kick and Kill Breakthrough

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How does Real-Time PCR work?

Here at ZAGENO, you can find kits for different templates, such as:

Genomic DNA

cDNA

mRNA

Total RNA

Real-time PCR (qPCR) kits will provide polymerases, oligonucleotides, and buffers for your experiments. Before comparing kits from different suppliers on our product page, though, there are a few factors to consider:

  • You should keep in mind the size of your amplicon. While in general qPCR amplifies short fragments of around 150 bases, there are polymerases with higher processivity. A side note: if you plan on performing Hot Start qPCR (prevents non-specific amplification at room temperature), look for kits including enzymes suitable for this application.

  • If you expect to amplify a template present in low quantities in your sample, consider kits that use SYTO 9 as an intercalating dye, which has higher sensitivity. For standard applications, SYBR Green I is still an excellent choice.

  • Alternatively, probe-based qPCR, use labeled fluorescent probes instead of intercalates. In doing so provides excellent sensitivity for sequence–specific detection, but need to be prepared specifically for your template beforehand.

  • If you are using RT-qPCR, make sure to pick a one- or two-step kit, according to your experimental setup.

ZAGENO's Real-Time PCR Troubleshoot will help guide you through any diffculties encountered.

Compare Real-Time PCR Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

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