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Sequencing on Zageno

PyroMark Q96 ID Software 2.5 QIAGEN
Product Application High-resolution quantification of di-, tri-, or tetra-allelic mutation; Genotyping and quantification of InDels, AQ, SNP, and CpG assays use sequence context as built-in quality control; Analysis of methylation in the presence of SNPs; Built-in quality control for bisulfite treatment in methylation assays; Base-calling with quality assessment Operating System No Windows 7 (32 bit) support Instrument Compatibility PyroMark Q96 ID systems
From (on request)
Sizes 1 (1 Software)
Catalog IDs 9022198
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Structural Multiplex Reference Standard Horizon Discovery
Sequencing Type Next Generation sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 480.00 (1 µg)
Sizes 1 (1 µg)
Catalog IDs HD753
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EGFR Gene-Specific Multiplex Reference Standard Horizon Discovery
Sequencing Type Next Generation sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 100.00 (1 FFPE Section)
Sizes 1 (1 FFPE Section)
Catalog IDs HD300
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Tru-Q 2 (5% Tier) Reference Standard Horizon Discovery
Sequencing Type Next Generation sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 240.00 (1 µg)
Sizes 1 (1 µg)
Catalog IDs HD729
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Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) GE Life Sciences
Sequencing Type Sanger sequencing Starting Material dsDNA Read Length /
From $ 885.00 (1000 Units)
Sizes 2 (1000 - 10000 Units)
Catalog IDs E79000Y, E79000Z
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VIC® Matrix Standard for the 310 Genetic Analyzer Applied Biosystems
Sequencing Type Sanger sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 45.99 (1 Tube)
Sizes 1 (1 Tube)
Catalog IDs 4313939
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Avadis® NGS Software Academic License Applied Biosystems
Product Application DNA Sequencing, Epigenetic Sequencing, Fragment Analysis, Ion Torrent™ Next-Generation Sequencing, RNA Sequencing Operating System Debian GNU, Mac OS X 10.5, Mac OS X 10.6, Mac OS X 10.7, Red Hat, Windows 7, Windows Vista, Windows XP Instrument Compatibility /
From (on request)
Sizes 1 (1 Software)
Catalog IDs 4467869
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NextGENe® Software for Ion Torrent™ Commercial/Network License Ion Torrent
Product Application DNA Sequencing, Epigenetic Sequencing, Fragment Analysis, Genotyping & Genomic Profiling, Ion Torrent™ Next-Generation Sequencing Operating System Windows 7, Windows Vista, Windows XP Instrument Compatibility Ion PGM™ System, Ion Proton™ System
From (on request)
Sizes 1 (1 Software)
Catalog IDs 4467743
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3500 Dx Genetic Analyzer 8-Capillary Array, 50 cm Applied Biosystems
Sequencing Type / Starting Material / Read Length /
From (on request)
Sizes 1 (1 Array)
Catalog IDs 4404684
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EpiNext™ Post-Bisulfite DNA Library Preparation Kit Epigentek
Sequencing Type Genome & DNA sequencing Starting Material ssDNA Final Product DNA library
From $ 462.00 (12 Reactions)
Sizes 2 (12 - 24 Reactions)
Catalog IDs P-1055-12, P-1055-24
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QIAseq 12-Index L QIAGEN
Sequencing Type Next Generation sequencing Starting Material Purified DNA Read Length /
From $ 1,089.00 (48 Reactions)
Sizes 1 (48 Reactions)
Catalog IDs 333764
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QIAseq 96-Index I Set D QIAGEN
Sequencing Type Next Generation sequencing Starting Material Purified DNA Read Length /
From $ 4,800.00 (384 Reactions)
Sizes 1 (384 Reactions)
Catalog IDs 333757
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Sequencing Kits and Methods

Library Preparation

Library Preparation is the first essential step for sequencing. Genomic library preparation is the basis for next generation sequencing.

Sequencing Kits

Sequencing has become more widespread as prices drop and efficiency as well as accuracy increase.

Sequencing Controls

Sequencing calibration is done using specific oligonucleotides with a known sequence and concentration; these sequences act as controls.

Lab Analysis Software

Analysis software reference samples to online databases, this is especially important as more databases are stored online.

Sequencing Chips

Sequencing chips are part of a semiconductor system which translates base-pair information into digital information.

DNA sequencing methods

DNA sequencing is likely the most frequently used sequencing application. As such, there are different DNA sequencing techniques.

Whole-genome sequencing constructs a complete analysis of an entire genome, or identifies mutations. This is an alternative to genome mapping, which is able to position genetic markers on the genome, i.e. the location of particular genes. Our Library Preparation Troubleshoot will help improve the efficiency of the initial stages of sequencing.

Historically, sequencing was done via the Sanger method of sequencing. With the advent of Next-Generation Sequencing (NGS), this technique is becoming increasingly efficient and easier to analyze. As for other experiments, the exact type of DNA and sample source is an important factor to consider,

Visit our Sanger Sequencing Troubleshoot for any problems you may be having in this area.

RNA sequencing

RNA Sequencing (whole transcriptome sequencing) is a gene expression analysis technique that employs NGS. It allows for the analysis of low expressing genes as well as alternative splicing or post transcriptional RNA modification. RNA sequencing can also be applied to miRNA or other non-coding RNAs, creating a powerful tool for RNA research. There are dedicated kits for each RNA target on the market, ready to make these experiments easy for you.

Our Next Generation Sequencing Troubleshoot is perfect for ironing out any discrepancies in your experiment.

Lab Analysis Software

When gathering large amounts of data, it is necessary to process it quickly and efficiently. That is where analysis programs come into play. Such analysis software can also be used to check your data against other databases (or even for identification purposes).

Specific software has been designed for primer design, restriction analysis and identification of genetic modifications, but to name a few.

Video and Image Credits

Videos:

BioNetwork/YouTube

C0nc0rdance/YouTube