ZAGENO
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Sequencing & Analysis Tools on ZAGENO

NEBNext® mRNA Library Prep Reagent Set for Illumina New England Biolabs
Sequencing Type Next Generation sequencing Starting Material mRNA Final Product /
From $ 675.00 (12 Reactions)
Sizes 2 (12 - 60 Reactions)
Catalog IDs E6100S, E6100L
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NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® New England Biolabs
Sequencing Type Next Generation sequencing Starting Material PCR products Final Product /
From $ 410.00 (12 Reactions)
Sizes 2 (12 - 60 Reactions)
Catalog IDs E6200S, E6200L
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Structural Multiplex Reference Standard Horizon Discovery
Sequencing Type Next Generation sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 480.00 (1 µg)
Sizes 1 (1 µg)
Catalog IDs HD753
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EGFR Gene-Specific Multiplex Reference Standard Horizon Discovery
Sequencing Type Next Generation sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 100.00 (1 FFPE Section)
Sizes 1 (1 FFPE Section)
Catalog IDs HD300
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Thermo Sequenase DNA Polymerase (with Thermoplasma acidophilum Inorganic Pyrophosphatase (TAP)) GE Life Sciences
Sequencing Type Sanger sequencing Starting Material dsDNA Read Length /
From $ 885.00 (1000 Units)
Sizes 2 (1000 - 10000 Units)
Catalog IDs E79000Y, E79000Z
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VIC® Matrix Standard for the 310 Genetic Analyzer Applied Biosystems
Sequencing Type Sanger sequencing Molecules for Standardization Standard sequences Standardization Type Standard
From $ 45.99 (1 Tube)
Sizes 1 (1 Tube)
Catalog IDs 4313939
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KAPA Library Amplification Kit With primer mix KAPA
Sequencing Type Genome & DNA sequencing, RNA sequencing Starting Material / Final Product /
From $ 809.25 (250 Reactions)
Sizes 1 (250 Reactions)
Catalog IDs KK2621
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3730/3730xl DNA Analyzer Sequencing Standards, BigDye® Terminator v3.1 Applied Biosystems
Sequencing Type Sanger sequencing Molecules for Standardization Standard sequences Standardization Type Instrument calibration, Spectral calibration
From $ 1,210.00 (4 Tubes)
Sizes 1 (4 Tubes)
Catalog IDs 4336943
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5500 SOLiD™ Fragment Library Barcode Adaptors 1-16 Applied Biosystems
Sequencing Type Genome & DNA sequencing Starting Material dsDNA Final Product Adapter-ligated DNA
From $ 1,116.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 4464405
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Image Lab™ Software Bio-Rad
Product Application Acquisition and analysis of gel and blot images Operating System Windows 7, 32- and 64-bit, and Windows 8.1, 64-bit; Mac OS X 10.8 and 10.9 Instrument Compatibility ChemiDoc™ MP, ChemiDoc, ChemiDoc XRS+, Gel Doc™ EZ, Gel Doc XR+, and GS-900™ Imaging Systems
From $ 3,820.00 (1 Software)
Sizes 1 (1 Software)
Catalog IDs 1709691
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GeneScan™ Installation Standard DS-33 [6FAM™, VIC®, NED™, PET™ dyes] Applied Biosystems
Sequencing Type Sanger sequencing Molecules for Standardization Labeled PCR products Standardization Type Installation standard, Size standard
From $ 422.00 (5 Tubes)
Sizes 1 (5 Tubes)
Catalog IDs 4330397
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310 Genetic Analyzer Matrix Standards, BigDye® Terminator v3.1 Applied Biosystems
Sequencing Type Sanger sequencing Molecules for Standardization Fluorescent dye Standardization Type Installation standard
From $ 111.00 (4 Tubes)
Sizes 1 (4 Tubes)
Catalog IDs 4336948
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DNA & RNA Sequencing

Sequencing is commonly associated with DNA sequencing, but it actually refers to any technique which determines the linear structure of macromolecules, such as peptide sequencing or RNA sequencing.

Sequencing Kits and Methods

Library Preparation

Library Preparation is the first essential step for sequencing. Genomic library preparation is the basis for next generation sequencing.

Sequencing Kits

Sequencing has become more widespread as prices drop and efficiency as well as accuracy increase.

Sequencing Controls

Sequencing calibration is done using specific oligonucleotides with a known sequence and concentration; these sequences act as controls.

Lab Analysis Software

Analysis software reference samples to online databases, this is especially important as more databases are stored online.

Sequencing Chips

Sequencing chips are part of a semiconductor system which translates base-pair information into digital information.

DNA sequencing methods

DNA sequencing is likely the most frequently used sequencing application. As such, there are different DNA sequencing techniques.

Whole-genome sequencing constructs a complete analysis of an entire genome, or identifies mutations. This is an alternative to genome mapping, which is able to position genetic markers on the genome, i.e. the location of particular genes. Our Library Preparation Troubleshoot will help improve the efficiency of the initial stages of sequencing.

Historically, sequencing was done via the Sanger method of sequencing. With the advent of Next-Generation Sequencing (NGS), this technique is becoming increasingly efficient and easier to analyze. As for other experiments, the exact type of DNA and sample source is an important factor to consider,

Visit our Sanger Sequencing Troubleshoot for any problems you may be having in this area.

RNA sequencing

RNA Sequencing (whole transcriptome sequencing) is a gene expression analysis technique that employs NGS. It allows for the analysis of low expressing genes as well as alternative splicing or post transcriptional RNA modification. RNA sequencing can also be applied to miRNA or other non-coding RNAs, creating a powerful tool for RNA research. There are dedicated kits for each RNA target on the market, ready to make these experiments easy for you.

Our Next Generation Sequencing Troubleshoot is perfect for ironing out any discrepancies in your experiment.

Lab Analysis Software

When gathering large amounts of data, it is necessary to process it quickly and efficiently. That is where analysis programs come into play. Such analysis software can also be used to check your data against other databases (or even for identification purposes).

Specific software has been designed for primer design, restriction analysis and identification of genetic modifications, but to name a few.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video Credits

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