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Library Preparation on ZAGENO

NEBNext® mRNA Library Prep Reagent Set for Illumina New England Biolabs
Sequencing Type Next Generation sequencing Starting Material mRNA Final Product /
From $ 675.00 (12 Reactions)
Sizes 2 (12 - 60 Reactions)
Catalog IDs E6100S, E6100L
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NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® New England Biolabs
Sequencing Type Next Generation sequencing Starting Material PCR products Final Product /
From $ 410.00 (12 Reactions)
Sizes 2 (12 - 60 Reactions)
Catalog IDs E6200S, E6200L
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KAPA Library Amplification Kit With primer mix KAPA
Sequencing Type Genome & DNA sequencing, RNA sequencing Starting Material / Final Product /
From $ 809.25 (250 Reactions)
Sizes 1 (250 Reactions)
Catalog IDs KK2621
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5500 SOLiD™ Fragment Library Barcode Adaptors 1-16 Applied Biosystems
Sequencing Type Genome & DNA sequencing Starting Material dsDNA Final Product Adapter-ligated DNA
From $ 1,116.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 4464405
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NEBNext® Ultra™ II Ligation Module New England Biolabs
Sequencing Type Next Generation sequencing Starting Material dsDNA Final Product Adapter-ligated DNA
From $ 395.00 (24 Reactions)
Sizes 2 (24 - 96 Reactions)
Catalog IDs E7595S, E7595L
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EpiNext™ Post-Bisulfite DNA Library Preparation Kit Epigentek
Sequencing Type Genome & DNA sequencing Starting Material ssDNA Final Product DNA library
From $ 462.00 (12 Reactions)
Sizes 2 (12 - 24 Reactions)
Catalog IDs P-1055-12, P-1055-24
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ClaSeek Library Preparation Kit, Illumina compatible Thermo Scientific
Sequencing Type Genome & DNA sequencing Starting Material dsDNA Final Product DNA library
From $ 397.00 (12 Reactions)
Sizes 2 (12 - 48 Reactions)
Catalog IDs K1341, K1342
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NEBNext® DNA Library Prep Master Mix Set for Illumina® New England Biolabs
Sequencing Type Next Generation sequencing Starting Material Genomic DNA Final Product /
From $ 350.00 (12 Reactions)
Sizes 2 (12 - 60 Reactions)
Catalog IDs E6040S, E6040L
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KAPA Single-Indexed Adapter Kit, Set A (30 µM) KAPA
From Not available
Sizes 1 (12 Adapters)
Catalog IDs KK8701
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NEBNext® Ultra™ RNA Library Prep Kit for Illumina® New England Biolabs
Sequencing Type Next Generation sequencing Starting Material Total RNA Final Product /
From $ 1,055.00 (24 Reactions)
Sizes 2 (24 - 96 Reactions)
Catalog IDs E7530S, E7530L
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NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1) New England Biolabs
Sequencing Type Next Generation sequencing Starting Material Total RNA Final Product /
From $ 1,470.00 (24 Reactions)
Sizes 2 (24 - 96 Reactions)
Catalog IDs E7300S, E7300L
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HID-Ion AmpliSeq Identity and Library Kit Applied Biosystems
Sequencing Type Genome & DNA sequencing Starting Material Genomic DNA Final Product DNA library
From $ 8,500.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs A26808
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Sequencing kits for different templates

Which kit you choose will largely depend on your starting material. Here at ZAGENO you can, amongst others, find:

  • cDNA library construction kits
  • Total RNA kits

See our Library Preparation Troubleshoot for solutions to any problems you may have.

Next generation sequencing

For Next Generation Sequencing there are many parameters to consider when creating a library. Once you know what kind of library you need, consider the size of the fragments. Short fragments can be amplified more efficiently while longer ones create better, more diffused sequencing clusters. The length of fragments might also be dictated by the nature of the template, the genome of interest, and the characteristics of its transcript.

There are also a few variables to think about for fragment creation. Physical DNA shearing methods such as sonication generally create fewer artifacts than enzymatic ones. But the latter are typically faster, reducing the overall sample preparation time.

RNA sequencing

For RNA sequencing applications, there are dedicated cDNA library kits available. Some kits maintain the original orientation of RNA in the cDNA copies,which is required for some applications. Others include only cDNA, avoiding DNA amplification from other RNA species, or to solve complex secondary RNA structures. Thus each kit can be tailored to fit your experimental parameters.

Compare Genomic DNA Library Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the NEXTERA DNA LIBRARY PREPARATION Kit from Illumina requires no more than 90 minutes.

  • that the QIASEQ FX DNA LIBRARY Kit from Qiagen is the cheapest per reaction.

  • that the PICO METHYL-SEQ™ LIBRARY PREP Kit requires as little as 10 pg of starting sample.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.