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Library Preparation on ZAGENO

DNA Library Prep Kit for Illumina Sequencing (Purification beads included) Applied Biological Materials
From $ 199.00 (12 Reactions)
Sizes 2 (12 - 48 Reactions)
Catalog IDs G947S, G947L
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EpiNext™ Post-Bisulfite DNA Library Preparation Kit Epigentek
Starting Material ssDNA Sequencing Type Genome & DNA sequencing Technology Adaptor Ligation
From $ 462.00 (12 Reactions)
Sizes 2 (12 - 24 Reactions)
Catalog IDs P-1055-12, P-1055-24
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KAPA Library Amplification Kit With primer mix KAPA
Starting Material / Sequencing Type Genome & DNA sequencing, RNA sequencing Technology /
From $ 809.25 (250 Reactions)
Sizes 1 (250 Reactions)
Catalog IDs KK2621
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TransNGS™ Library Dilution Buffer TransGen Biotech
Starting Material / Sequencing Type / Technology /
From $ 92.31 (5 ml)
Sizes 1 (5 ml)
Catalog IDs KB101-01
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Ion AmpliSeq™ Cancer Hotspot Panel v2 Ion Torrent
Starting Material Blood, Cells, Genomic DNA, Tissue, dsDNA Sequencing Type Genome & DNA sequencing Technology Amplicon Sequencing, Post-Light™ Ion Semiconductor Sequencing
From $ 266.00 (8 Reactions)
Sizes 1 (8 Reactions)
Catalog IDs 4475346
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5500 SOLiD™ Fragment Library Barcode Adaptors 1-16 Applied Biosystems
Starting Material dsDNA Sequencing Type Genome & DNA sequencing Technology Library barcoding
From $ 1,240.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 4464405
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ClaSeek Library Preparation Kit, Illumina compatible Thermo Scientific
Starting Material dsDNA Sequencing Type Genome & DNA sequencing Technology Thermo Scientific ClaSeek library construction technology
From $ 405.00 (12 Reactions)
Sizes 2 (12 - 48 Reactions)
Catalog IDs K1341, K1342
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Pico Methyl-Seq™ Library Prep Kit Zymo Research
Starting Material FFPE tissue, Genomic DNA Sequencing Type Next Generation sequencing Technology Amplification
From $ 680.00 (10 Reactions)
Sizes 2 (10 - 25 Reactions)
Catalog IDs D5455, D5456
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DNA SMART™ ChIP-Seq Kit Clontech
Starting Material Purified DNA, dsDNA, ssDNA Sequencing Type Next Generation sequencing Technology Amplification
From $ 588.00 (12 Reactions)
Sizes 3 (12 - 48 Reactions)
Catalog IDs 634865, 634866, ...
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EpiNext™ DNA Size Selection Kit Epigentek
Starting Material dsDNA Sequencing Type Next Generation sequencing Technology Magnetic bead
From $ 99.00 (48 Reactions)
Sizes 2 (48 - 96 Reactions)
Catalog IDs P-1059-48, P-1059-96
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TransNGS™ Tn5 Index Kit for Illumina TransGen Biotech
Starting Material / Sequencing Type / Technology /
From $ 61.53 (1 Kit)
Sizes 2 (1 Kit)
Catalog IDs KI101-01, KI101-02
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TransNGS™ Library Quantification Kit for Illumina TransGen Biotech
Starting Material / Sequencing Type / Technology /
From $ 169.23 (100 Reactions)
Sizes 2 (100 - 500 Reactions)
Catalog IDs KQ101-01, KQ101-02
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Sequencing kits for different templates

Which kit you choose will largely depend on your starting material. Here at ZAGENO you can, amongst others, find:

  • cDNA library construction kits
  • Total RNA kits

See our Library Preparation Troubleshoot for solutions to any problems you may have.

Next generation sequencing

For Next Generation Sequencing there are many parameters to consider when creating a library. Once you know what kind of library you need, consider the size of the fragments. Short fragments can be amplified more efficiently while longer ones create better, more diffused sequencing clusters. The length of fragments might also be dictated by the nature of the template, the genome of interest, and the characteristics of its transcript.

There are also a few variables to think about for fragment creation. Physical DNA shearing methods such as sonication generally create fewer artifacts than enzymatic ones. But the latter are typically faster, reducing the overall sample preparation time.

RNA sequencing

For RNA sequencing applications, there are dedicated cDNA library kits available. Some kits maintain the original orientation of RNA in the cDNA copies,which is required for some applications. Others include only cDNA, avoiding DNA amplification from other RNA species, or to solve complex secondary RNA structures. Thus each kit can be tailored to fit your experimental parameters.

Compare Genomic DNA Library Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the NEXTERA DNA LIBRARY PREPARATION Kit from Illumina requires no more than 90 minutes.

  • that the QIASEQ FX DNA LIBRARY Kit from Qiagen is the cheapest per reaction.

  • that the PICO METHYL-SEQ™ LIBRARY PREP Kit requires as little as 10 pg of starting sample.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.