Library Preparation is the first essential step for sequencing experiments. While cDNA library construction is still used for transcriptome studies, genomic library preparation is the basis for next generation sequencing techniques such as whole genome sequencing or RNA-seq. Sometimes the sequencing kits include the cDNA library preparation step, so don’t forget to check if this is the case for your sequencing kit of choice!
Sequencing kits for different templates
Which kit you choose will largely depend on your starting material. Here at ZAGENO you can, amongst others, find:
cDNA library construction kits
Total RNA kits
See our Library Preparation Troubleshoot for solutions to any problems you may have.
Next generation sequencing
For Next Generation Sequencing there are many parameters to consider when creating a library. Once you know what kind of library you need, consider the size of the fragments. Short fragments can be amplified more efficiently while longer ones create better, more diffused sequencing clusters. The length of fragments might also be dictated by the nature of the template, the genome of interest, and the characteristics of its transcript.
There are also a few variables to think about for fragment creation. Physical DNA shearing methods such as sonication generally create fewer artifacts than enzymatic ones. But the latter are typically faster, reducing the overall sample preparation time.
For RNA sequencing applications, there are dedicated cDNA library kits available. Some kits maintain the original orientation of RNA in the cDNA copies,which is required for some applications. Others include only cDNA, avoiding DNA amplification from other RNA species, or to solve complex secondary RNA structures. Thus each kit can be tailored to fit your experimental parameters.
Compare Library Preparation Kits
With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.
The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.