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Sequencing Controls

Score 0.94

Novagen™ Mouse Genomic DNA

MilliporeSigma

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 176.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
69239-3
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Sequencing Controls

Score 0.10

Novagen™ NovaQUANT™ Human Mitochondrial to Nuclear DNA Ratio Kit

MilliporeSigma

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 351.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
72620-1KIT
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Sequencing Controls

Score 8.59

Structural Multiplex Reference Standard

Horizon Discovery

Sequencing Type
Next Generation sequencing
Next Generation sequencing
Molecules for Standardization
Standard sequences
Standard sequences
Standardization Type
Standard
Standard
From
$ 480.00 (1 µg)
Sizes
1 (1 µg)
Catalog IDs
HD753
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Sequencing Controls

Score 9.22

EGFR Gene-Specific Multiplex Reference Standard

Horizon Discovery

Sequencing Type
Next Generation sequencing
Next Generation sequencing
Molecules for Standardization
Standard sequences
Standard sequences
Standardization Type
Standard
Standard
From
$ 100.00 (1 FFPE Section)
Sizes
1 (1 FFPE Section)
Catalog IDs
HD300
Added to comparison remove item

Sequencing Controls

Score 8.91

Tru-Q 2 (5% Tier) Reference Standard

Horizon Discovery

Sequencing Type
Next Generation sequencing
Next Generation sequencing
Molecules for Standardization
Standard sequences
Standard sequences
Standardization Type
Standard
Standard
From
$ 240.00 (1 µg)
Sizes
1 (1 µg)
Catalog IDs
HD729
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Sequencing Controls

Score 1.09

Herring Sperm DNA (5 x 1 mL)

Novus Biologicals

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 99.00 (5 ml)
Sizes
1 (5 ml)
Catalog IDs
9605-5-D
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Sequencing Controls

Score 1.33

Plesiomonas shigelloides Z130, Genomic DNA, SKU: 0801899DNA10UG

ZeptoMetrix

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 425.69 (10 Units)
Sizes
1 (10 Units)
Catalog IDs
0801899DNA10UG
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Sequencing Controls

Score 1.33

Vibrio cholerae Z132, Genomic DNA, SKU: 0801901DNA10UG

ZeptoMetrix

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 443.00 (10 Units)
Sizes
1 (10 Units)
Catalog IDs
0801901DNA10UG
Added to comparison remove item

Sequencing Controls

Score 0.70

Novagen™ Rat Genomic DNA

MilliporeSigma

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 186.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
69238-3
Added to comparison remove item

Sequencing Controls

Score 2.03

Novagen™ NovaQUANT™ Mouse Mitochondrial to Nuclear Ratio kit

MilliporeSigma

Sequencing Type
/
/
Molecules for Standardization
/
/
Standardization Type
/
/
From
$ 474.89 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
72621-1KIT
Added to comparison remove item

Sequencing Controls

Score 2.19

Kapa BiosystemsSupplier Diversity Partner DNS STANDARDS

KAPA

From
$ 308.10 (480 µl)
Sizes
1 (480 µl)
Catalog IDs
KK4903-07960387001
Added to comparison remove item

Sequencing Controls

Score 8.75

Tru-Q 1 (5% Tier) Reference Standard

Horizon Discovery

Sequencing Type
Next Generation sequencing
Next Generation sequencing
Molecules for Standardization
Standard sequences
Standard sequences
Standardization Type
Standard
Standard
From
$ 240.00 (1 µg)
Sizes
1 (1 µg)
Catalog IDs
HD728
Added to comparison remove item

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Whichever sequencing techniques your lab employs, the efficiency and precision of your sequencing data analysis depends on the sequencing machine used and in turn on its calibration. Sequencing calibration is done using specific oligonucleotides with a known sequence and concentration; these sequences act as controls (also known as standards). Test sequences can then be compared against controls, so as to initially estimate values such as length, mass, and concentration.

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Using Controls

Sequencing is a central technique in any modern molecular biology lab. Cycle DNA sequencing is routinely used for checking the fidelity of cloning experiments, while next generation sequencing is becoming the new standard for genome analysis and genotyping. Moreover, RNA-seq has established itself as an alternative to conventional expression analysis.

To ensure successful experimentation, calibration sequencing controls should be run every time:

You run a new assay on your system

You adjust your camera or sensors

You notice a decrease in the quality of spectral separation.

For NGS systems, software tools like fastqc and ngs qc toolkit can evaluate the quality of nucleotide sequences, hinting whether a recalibration is needed.

Each sequencer supplier will provide standard kits specific for your system, whether you perform nucleotide or protein sequence analysis. The oligonucleotide controls can be labelled with a dye, which may be fluorescent, although some are just known, standard, sequences.

Compare Sequencing Controls

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.