Whichever sequencing techniques your lab employs, the efficiency and precision of your sequencing data analysis depends on the sequencing machine used and in turn on its calibration. Sequencing calibration is done using specific oligonucleotides with a known sequence and concentration; these sequences act as controls (also known as standards). Test sequences can then be compared against controls, so as to initially estimate values such as length, mass, and concentration.
Sequencing is a central technique in any modern molecular biology lab. Cycle DNA sequencing is routinely used for checking the fidelity of cloning experiments, while next generation sequencing is becoming the new standard for genome analysis and genotyping. Moreover, RNA-seq has established itself as an alternative to conventional expression analysis.
To ensure successful experimentation, calibration sequencing controls should be run every time:
You run a new assay on your system
You adjust your camera or sensors
You notice a decrease in the quality of spectral separation.
For NGS systems, software tools like fastqc and ngs qc toolkit can evaluate the quality of nucleotide sequences, hinting whether a recalibration is needed.
Each sequencer supplier will provide standard kits specific for your system, whether you perform nucleotide or protein sequence analysis. The oligonucleotide controls can be labelled with a dye, which may be fluorescent, although some are just known, standard, sequences.
Compare Sequencing Controls
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The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between
researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows
you to make an informed decision with minimum effort.