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Immunodetection

Introduction

Immunodetection includes any assay technique used to detect and quantify amino acids, antibodies, and hormones. The most common forms are ELISA and Western Blots. Make sure that you have as clean samples as possible before you start using these methods as sample purity greatly affects the downstream outcome. Have a look at our Protein Concentration and Quantification categories to ensure successful protein purification.

ELISA

Enzyme-linked Immunosorbent Assay utilizes antibodies to detect molecules via simple color change. This is done by applying antibodies to a surface (usually a well plate), then introducing your sample to these antibodies. If your target molecule is within the sample, then it will bind to the antibody. Next, another antibody, specific to your molecule, is added but with a detection enzyme attached. When this enzyme's substrate is added, those antibodies attached to your molecule will cause the liquid to change color, thus allowing for specific detection. If you are experiencing problems, then checkout our ELISA troubleshooting guide.

Western Blot

Western Blot is another immunodetection technique used for detection of proteins. Blotting refers to the process of moving biological samples from a gel to a membrane, with detection steps being performed on the surface of the membrane. The chromatographic properties of PAGE electrophoresis are used to separate macromolecules, which are then transferred (i.e. blotted) to a membrane, usually through physical and electrical methods. Next, the membrane is probed with antibodies specific to your molecule of choice. Secondary antibodies, which are bound to a detector enzyme, are then added. Following the same procedure as ELISA, the enzyme's substrates will change the color of the membrane in the presence of your target molecule. Otherwise the protein can be transferred to a photographic film and visualized by chemiluminescence via the reporter on the secondar antibody.

Here at ZAGENO, you have the unique possibility to compare the products from different suppliers to find the most suitable one for your experiment.

Compare Immunodetection Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.