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Molecular Biology Methods vary widely and range from DNA Extraction to Real-Time PCR to the newly-discovered gene editing technique CRISPR. Zageno has composed a selection of articles detailing the technologies and mechanisms that make up life science research.
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Preparation generally pertains to the extraction and purification of proteins and/or nucleic acids. It is key to the success of downstream applications like PCR, as contamination of your DNA sample will greatly hinder the reaction.
Gene Editing involves the insertion, deletion / silencing, or replacement of DNA sequences within a genome. Genetic modification is important in analyzing gene expression and function, but also has great therapeutic potential for Huntington's disease and certain types of cancer, but to name a few.
The Polymerase Chain Reaction (PCR) is an in vitro method used for the amplification of a specific DNA template; generating a large number of copies in just a few hours.
Sequencing is commonly associated with DNA, but it actually refers to any technique which determines the linear structure of macromolecules, such as peptide sequencing or RNA sequencing.
Detection for the presence and/or concentration of nucleic acids, peptides, antibodies and antigens etc. is a huge part of modern research. Methods of detection include immunoassays, quantification through fluorophores and radiolabeling.
Accessories are key components in a wide variety of experiments and the choice of accessory is quite often based on the preferences of the individual researcher.