RNA stabilization and preservation
Revolution of RNA Stabilization
RNA stabilization is essential for successful gene expression analysis, as RNAses quickly degrade RNA, causing misleading results such as faulty expression profiles. Prevention of RNA usually requires freezing the sample to -80 °C in the lab, but the transportation and energy requirements of a 150 kg freezer is often an issue when conducting fieldwork. Thus, alternative methods have been developed allowing expression profiles of, for example, natural communities of ponds, oceans, jungles and ice fields, or wherever your scientific journey takes you.
RNA stabilizing solutions changed the game when they appeared on the market; enabling an insight into gene expression in vivo that had not been possible before.
Here you can have a look at a few of these pretty nifty products and compare them side-by-side:
RNA stabilization and downstream processes
RNA stabilized in solution does not hinder downstream applications such as DNA or protein purification. However, proteins can denature, making RNA stabilization incompatible with methods requiring native proteins. Despite this, Western blotting and 2D gel electrophoresis are still compatible.
Approximate storage length of the RNA samples is 1-7 days at room temperature, about one month at 4 °C, and a year or more at -20 to -80 °C.