Specifications

Variant Details
Catalog ID K-6603 Vendor Catalog ID K-6603
Size 250 Reactions Price $ 127.62
Vendor Bioneer Corporation Package Content 2X Master mix 2.5 ml DEPC-DW 1.2 ml 50X ROX 0.1 ml Instruction manual
General
PCR Type One-step PCR Hot-Start PCR Hot-start PCR
Multiplex PCR Multiplex PCR Reference Dye ROX
Fluorescent Dye Included Templates Genomic DNA, Plasmid DNA, Viral DNA, cDNA, dsDNA
Template Amount 10 pg - 100 ng Polymerase Hotstart Taq DNA Polymerase
Amplicon Size < 200 bp Reaction Volume 20 µl
Instrument Compatibility Any qPCR machine Sample Type Cell-free sample, Lysate, Purified DNA
Organism Animals, Bacteria, Human, Plants, Viruses Elongation Temperature 72 °C
Fidelity No proofreading activity Activation Step 5 min at 95 °C
Concentration 2X Master mix Product Application qPCR
Downstream Processes Gel electrophoresis, restriction enzyme digestion, sequencing Lyophilized Not lyophilized
Previous Brand / Trademark DualStar™ Shipping Condition Dry ice
Shipping Temperature -20 °C Storage & Handling -20 °C
Expiry Time 24 months Research Topics Genetics, Genomics, Molecular biology
Low Hazard Product No High Hazard Product No

Description

AccuPower Plus DualStar™ qPCR 2X Master Mix for Real-Time PCR – Bioneer


Bioneer’s AccuPower Plus DualStar™ qPCR 2X Master Mix is designed for qPCR (quantitative polymerase chain reaction) with Genomic DNA, Plasmid DNA, Viral DNA, cDNA, dsDNA, and other tested templates, via One-step PCR, and a dedicated Hotstart Taq DNA Polymerase.
Due to Bioneer protocol and components, this research kit has a Hot-start PCR and Multiplex PCR classification.


Real-Time PCR Reaction Volume & Amplicon Size


Amplicons of < 200 bp can be produced from 10 pg - 100 ng, as part of a 20 µl reaction volume. These specifications are facilitated by Bioneer’s included enzyme: Hotstart Taq DNA Polymerase, with an activation requirement of 5 min at 95 °C.
This Bioneer Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with ROX included as a corresponding reference dye. As a result, this research product is compatible with the Any qPCR machine, and other specified instruments.


Applications & Downstream Experiments


The AccuPower Plus DualStar™ qPCR 2X Master Mix is suitable for qPCR, among other research experiments and is compatible with Gel electrophoresis, restriction enzyme digestion, sequencing or other tested methods.


qPCR Troubleshooting Tips


Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (72 °C). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.

For research use only.

Resources

Protocol

AccuPower Plus DualStar™ qPCR 2X Master Mix

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Protocol

AccuPower Plus DualStar™ qPCR 2X Master Mix

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