AccuPower Plus DualStar™ qPCR 2X Master Mix for Real-Time PCR – Bioneer
Bioneer’s AccuPower Plus DualStar™ qPCR 2X Master Mix is designed for qPCR (quantitative polymerase chain reaction) with Genomic DNA, Plasmid DNA, Viral DNA, cDNA, dsDNA, and other tested templates, via One-step PCR, and a dedicated Hotstart Taq DNA Polymerase.
Due to Bioneer protocol and components, this research kit has a Hot-start PCR and Multiplex PCR classification.
Real-Time PCR Reaction Volume & Amplicon Size
Amplicons of < 200 bp can be produced from 10 pg - 100 ng, as part of a 20 µl reaction volume. These specifications are facilitated by Bioneer’s included enzyme: Hotstart Taq DNA Polymerase, with an activation requirement of 5 min at 95 °C.
This Bioneer Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with ROX included as a corresponding reference dye. As a result, this research product is compatible with the Any qPCR machine, and other specified instruments.
Applications & Downstream Experiments
The AccuPower Plus DualStar™ qPCR 2X Master Mix is suitable for qPCR, among other research experiments and is compatible with Gel electrophoresis, restriction enzyme digestion, sequencing or other tested methods.
qPCR Troubleshooting Tips
Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (72 °C). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.