Description

AmpliTaq Gold® 360 Master Mix for End-Point PCR – Applied Biosystems


Applied Biosystems’s AmpliTaq Gold® 360 Master Mix utilizes the enzyme AmpliTaq Gold 360 DNA Polymerase, to amplify Genomic DNA, cDNA, and similar templates. Included PCR reagents and buffers help support regular PCR in addition to singleplex PCR, allowing complete control over amplification specificity (i.e., specific or non-specific amplification) and complexity (single or multiple target amplification).


Polymerase and Protocol Specifications


The AmpliTaq Gold 360 DNA Polymerase is supplied at the specified concentration and is activated by conditions stated in the protocol during denaturing. Following annealing, nucleic acid samples of the suggested weight or volume are elongated at 72 °C. Applied Biosystems recommends a 50 µl PCR reaction volume and amplicons of < 5 kb for optimal polymerase performance. Utilizing thermal cycling and regular PCR to replicate DNA, AmpliTaq Gold 360 DNA Polymerase extends primers with free dNTPs, forming a complementary second strand.


Applications & Downstream Experiments of AmpliTaq Gold® 360 Master Mix


Intended uses of Applied Biosystems’s AmpliTaq Gold® 360 Master Mix include Amplification of low-copy amplicons and long targets, PCR, as well as related PCR cloning and amplification techniques. The resulting product is applicable for use in sequencing, and other tested methods.


PCR Troubleshooting Tips


Contamination and inappropriate primer construction create amplification inefficiencies. Therefore, PCR cleanup kits enhance the purity of either the starting or final sample, maximizing accuracy, and effectiveness. Dedicated primer design tools may also be useful in those respects. Optimum temperatures suggested by Invitrogen should be enforced, e.g., elongation (72 °C). Fidelity and enzyme concentration (see product specifications) also need to be accounted for when setting up your PCR.

For research use only.

Specifications

TemplatesGenomic DNA, cDNA
OrganismHuman
Product ApplicationAmplification of low-copy amplicons and long targets, PCR
PolymeraseAmpliTaq Gold 360 DNA Polymerase
Elongation Temperature72 °C
Amplicon Size< 5 kb
Template Amount< 1 µg
Reaction Volume50 µl
Shipping ConditionDry ice, Wet ice
Storage TemperatureStorage: -15°C to -25°C. Before use, thaw frozen components to room temperature and mix well.
Package Content AmpliTaq Gold 360 Master Mix 2X 360 GC Enhancer
Variant Details Size: 1 Unit Format Non-Plated Catalog ID 4398876 Price $ 124.00

Resources

Handbook

Quick Ref: AmpliTaq Gold® 360 Master Mix (English )
tools.thermofisher.com PDF
Protocol: AmpliTaq Gold® 360 Master Mix (English )
tools.thermofisher.com PDF
Protocol: AmpliTaq Gold 360 Master Mix
tools.thermofisher.com PDF
Product Bulletin: AmpliTaq Gold® 360 Master Mix and 360 DNA Polymerase
tools.thermofisher.com PDF
Product Insert: AmpliTaq Gold 360 Master Mix
tools.thermofisher.com PDF