Description

AmpliTaq Gold® 360 Master Mix for End-Point PCR – Applied Biosystems


Applied Biosystems’s AmpliTaq Gold® 360 Master Mix utilizes the enzyme AmpliTaq Gold 360 DNA Polymerase, to amplify Genomic DNA, cDNA, and similar templates. Included PCR reagents and buffers help support Hot-start PCR in addition to Multiplex PCR, allowing complete control over amplification specificity (i.e., specific or non-specific amplification) and complexity (single or multiple target amplification).


Polymerase and Protocol Specifications


The AmpliTaq Gold 360 DNA Polymerase is supplied at the specified concentration and is activated by conditions stated in the protocol during denaturing. Following annealing, nucleic acid samples of the suggested weight or volume are elongated at 72 °C. Applied Biosystems recommends a 50 µl PCR reaction volume and amplicons of < 5 kb for optimal polymerase performance. Utilizing thermal cycling and Hot-start PCR to replicate DNA, AmpliTaq Gold 360 DNA Polymerase extends primers with free dNTPs, forming a complementary second strand.


Applications & Downstream Experiments of AmpliTaq Gold® 360 Master Mix


Intended uses of Applied Biosystems’s AmpliTaq Gold® 360 Master Mix include PCR, Amplification of low-copy amplicons and long targets, as well as related PCR cloning and amplification techniques. The resulting product is applicable for use in sequencing, and other tested methods.


PCR Troubleshooting Tips


Contamination and inappropriate primer construction create amplification inefficiencies. Therefore, PCR cleanup kits enhance the purity of either the starting or final sample, maximizing accuracy, and effectiveness. Dedicated primer design tools may also be useful in those respects. Optimum temperatures suggested by Invitrogen should be enforced, e.g., elongation (72 °C). Fidelity and enzyme concentration (see product specifications) also need to be accounted for when setting up your PCR.

For research use only.

Specifications

Hot-Start PCRHot-start PCR
Multiplex PCRMultiplex PCR
TemplatesGenomic DNA, cDNA
Template Amount< 1 µg
PolymeraseAmpliTaq Gold 360 DNA Polymerase
FidelityProofreading activity
Amplicon Size< 5 kb
Reaction Volume50 µl
OrganismHuman
Elongation Temperature72 °C
Product ApplicationPCR, Amplification of low-copy amplicons and long targets
Shipping ConditionDry ice, Wet ice
Storage Temperature-20 °C
Low Hazard ProductYes
High Hazard ProductYes
LyophilizedLyophilized
Package Content AmpliTaq Gold 360 Master Mix 2X 360 GC Enhancer
Variant Details Size: 40 Reactions Format Non-Plated Catalog ID 4398876 Price $ 124.00

Resources

Handbook

Quick Ref: AmpliTaq Gold® 360 Master Mix (English )
tools.thermofisher.com PDF
Protocol: AmpliTaq Gold® 360 Master Mix (English )
tools.thermofisher.com PDF
Protocol: AmpliTaq Gold 360 Master Mix
tools.thermofisher.com PDF
Product Bulletin: AmpliTaq Gold® 360 Master Mix and 360 DNA Polymerase
tools.thermofisher.com PDF
Product Insert: AmpliTaq Gold 360 Master Mix
tools.thermofisher.com PDF

Discussion

by sitti , 11 months, 2 weeks ago

i would like to ask the price for the AMPLITAQ GOLD 360 MASTER MIX ,AmpliTaq Gold® 360 PCR Master Mix contains everything required for PCR amplification, supplied at 2X concentration, main ingredient is AmpliTaq Gold® 360 DNA Polymerase (Catalog Number: 43988810)