Specifications

Variant Details
Catalog IDB0807-13U-100UGSupplier Catalog IDB0807-13U
Size100 µgPrice $ 633.00
Supplier United States Biological Inc. Package Content Anti-Bcl-w, clone 6D159
General
ClonalityMonoclonalHostRat
ReactivityHumanCross ReactivityCanine, Human, Monkey, Monkey, Rat
ApplicationsImmunoprecipitation, Western BlotClone No.6D159
IsotypeIgGImmunogenRecombinant protein corresponding to human Bcl-w
Purification MethodPurified by Protein G affinity chromatography.GeneBcl w
UniProtKBQ92843FormLiquid
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage BufferPBS, pH 7.4, 0.02% sodium azide, 25% glycerol.PurityAffinity Purified

Description

Bcl-w is a 21kD homolog of bcl-2 (1). It is an apoptosis inhibitor that is implicated in the development of follicular lymphoma in human (1). The structural gene of Bcl-w is located on chromosome 14 of mouse, near the T-cell antigen receptor alpha gene. Enforced expression of Bcl-w enables cells to withstand several cytotoxic conditions, suggesting that this protein promotes cell survival. Mutant mice that failed to produce bcl-w exhibit sterility associated with progressive testicular degeneration (2). In young adults, spermatogenesis is blocked during late spermiogenesis. This is followed by gradual depletion of all stages of germ cells, with the exception of Sertoli cells. Finally all Sertoli cells are lost from the seminiferous tubules (2).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Western Blot: Works for transfected and Bcl-w overproducing cells. For detection of endogemous Bcl-w, Immunoprecipitation is required.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only.