Specifications

Variant Details
Catalog IDC2255-01J-100UGSupplier Catalog IDC2255-01J
Size100 µgPrice $ 593.00
Supplier United States Biological Inc. Package Content Anti-CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4)
General
ClonalityMonoclonalHostMouse
ReactivityFelineCross ReactivityFeline
ApplicationsELISA, Immunocytochemistry, Western blotClone No.7H154
IsotypeIgGImmunogenNS0-derived recombinant feline CD4 (aa25-413) Accession # BAA19124
Purification MethodPurified by Protein G affinity chromatography.GeneCD4
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CStorage BufferPBS, 5% trehalose.
PurityAffinity Purified

Description

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunocytochemistry: 25ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.