Anti-CDC42 Rabbit Monoclonal Antibody tested for Flow Cytometry, IP, IHC, WB in Human, Mouse, Rat. Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome. Protein Function: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.
|Catalog ID||M00119-100UG||Supplier Catalog ID||M00119|
|Size||100 µg||Price||$ 299.00|
|Supplier||BosterBio||Package Content||Anti-CDC42 Rabbit Monoclonal Antibody|
|Reactivity||Human, Mouse, Rat||Applications||Immunoprecipitation, Western Blot|
|Immunogen||A synthesized peptide derived from human CDC42||Concentration||0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.|
|Dilution||WB 1:1000-1:2000 IHC 1:50-1:200 IP 1:30 FC 1:50||Formulation||Phosphate Buffered Saline , Ph 7.4, 150Mm Nacl, 0.02% Sodium Azide And 50% Glycerol|
|Purification Method||Affinity-chromatography||Gene||Cell division control protein 42 homolog (CDC42)|
|Alternative Names & Synonyms||Cell division control protein 42 homolog;G25K GTP-binding protein;CDC42||UniProtKB||P60953|
|Molecular Weight||21259 MW||Form||Liquid|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.||Free Sample / Trial||Free Sample Available|
For research use only.