Specifications

Variant Details
Catalog IDC5042-20A-100UGSupplier Catalog IDC5042-20A
Size100 µgPrice $ 593.00
Supplier United States Biological Inc. Package Content Anti-Cholecystokinin A Receptor (CCKA Receptor, CCKAR, AW106902)
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsFlow cytometryClone No.7H167
IsotypeIgGImmunogenRecombinant protein corresponding to human Cholecystokinin A Receptor expressed in NSO cells (NP_000721).
Purification MethodPurified by Protein G affinity chromatography from cell culture supernatant.GeneCholecystokinin A Receptor
UniProtKBP32238Protein Accession No.NP_000721.1
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CStorage BufferPBS, 5% trehalose.
PurityAffinity Purified

Description

Cholecystokinin A Receptor (CCKAR) is one of two 7TM receptors for cholecystokinin peptide hormones. This GPCR is involved in gallbladder contraction, insulin secretion, and central nervous system function. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction. Human CCK-A R shares 89% aa sequence identity with mouse and rat CCKAR.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 25ug/ml, 10ul of the diluted solution labels 1-2.5x10e5 cells in a total reaction volume ≤200ul. Validated for use using human CCKAR transfectants.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 

For research use only.