Specifications

Variant Details
Catalog IDC9097-29-50UGSupplier Catalog IDC9097-29
Size50 µgPrice $ 752.00
Supplier United States Biological Inc. Package Content Anti-Cytokeratin-20, clone 5F215
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman, Monkey, Pig, Rat
ApplicationsELISA, Immunohistochemistry, Western blotClone No.5F215
IsotypeIgGImmunogenElectrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa.
Purification MethodPurified by Protein A affinity chromatography.GeneCytokeratin 20
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CStorage BufferPBS, pH 7.4, 0.5% BSA and 0.09% sodium azide.
PurityAffinity Purified

Description

Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.

Applications:
Suitable for Immunohistochemistry (frozen tissue paraffin sections only after microwave treatment), Immunoblot (Western) and ELISA.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.