Specifications

Variant Details
Catalog IDI8427-02A-250UGSupplier Catalog IDI8427-02A
Size250 µgPrice $ 805.00
Supplier United States Biological Inc. Package Content Anti-Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin)
General
ClonalityMonoclonalHostMouse
ReactivityFelineCross ReactivityFeline
Applicable ProcessesELISAClone No.11n97
ConjugatedConjugatedConjugate TypeBiotin
IsotypeIgGImmunogenRecombinant corresponding to aa20-134 from feline IL-5 expressed in NSO cells (O77515).
Purification MethodPurified by Protein A/G affinity chromatography.GeneInterleukin 5
UniProtKBO77515FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage BufferPBS, BSA.PurityAffinity Purified

Description

Interleukin-5 (IL-5) is a 40kD secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the a-helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115aa mature feline IL-5 shares 70-59aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding a subunit with a signal transducing common b subunit (bc) that is shared with receptor complexes for IL-3 and GM-CSF.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 0.5-2ug/ml
ELISA (capture): 2-8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.