Specifications

Variant Details
Catalog IDI8430-22C-500UGSupplier Catalog IDI8430-22C
Size500 µgPrice $ 729.00
Supplier United States Biological Inc. Package Content Anti-Interleukin 8 SKU: I8430-22C
General
ClonalityMonoclonalHostRat
ReactivityFelineCross ReactivityFeline
Applicable ProcessesELISA, NeutralizationClone No.9H141
IsotypeIgGImmunogenRecombinant feline CXCL8, aa23-101; Q9XSX5 (E. coli).
Purification MethodPurified by Protein G affinity chromatography.GeneInterleukin 8
UniProtKBQ9XSX5FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
Storage Buffer0.2um sterile-filtered solution, PBS, 5% trehalose.PurityAffinity Purified

Description

CXCL8, also known as IL-8, is a chemoattractant that recruits neutrophils to sites of inflammation, activates neutrophil functions and promotes angiogenesis. CXCL8 production is rapidly induced by pro-inflammatory signals in many different cell types. The biological activites of CXCL8 are mediated by chemokine receptors CXCR1 and CXCR2. Feline CXCL8 is translated as a 101 aa precursor protein with a 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine CXCL8, respectively.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 2-8ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.