Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.
Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.
Immunohistochemistry: 8-25ug/ml. Detects Substance P in paraffin-embedded human spinal cord tissue sections when used with the appropriate secondary reagents.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.