Specifications

Variant Details
Catalog IDS8000-01-100UGSupplier Catalog IDS8000-01
Size100 µgPrice $ 608.00
Supplier United States Biological Inc. Package Content Anti-Substance P (NK, Neurokinin)
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman, Monkey, Rat
ApplicationsELISA, ImmunohistochemistryClone No.7H63
IsotypeIgGImmunogenKLH-conjugate of Substance P, (RPKPQQFFGLM).
Purification MethodPurified by Protein G affinity chromatography.GeneSubstance P
FormLyophilizedShipping TemperatureBlue Ice
Storage & Handling-20°CStorage BufferPBS, 5% trehalose.
PurityAffinity Purified

Description

Substance P (Neurokinin-1), is member of the tachykinin peptide family. Processing of preprotachykinin isoforms generates Substance P and other tachykinins. Active Substance P is an 11 amino acid peptide, RPKPQQFFGLM, that is identical across all mammalian species. Substance P is mainly secreted from C-type fibers of primary sensory afferent neurons after stimulation through protease-activated receptor 2 (PAR2). Substance P binds primarily to the neurokinin 1 receptor on neurons in the dorsal horn, transmitting pain signals through the central nervous system.

Applications:
Suitable for use in Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml. Detects Substance P in paraffin-embedded human spinal cord tissue sections when used with the appropriate secondary reagents.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.