Specifications

Variant Details
Catalog ID 95024-146 Vendor Catalog ID C838X90
Size 1 Unit Price $ 753.72
Vendor Thomas Scientific LLC Package Content BIGEASY-TSA ELECTROCOMP CELL 24RXN
General
Competence Electro

Description

BigEasy®-TSA™ Electrocompetent Cells – VWR International


VWR International’s BigEasy®-TSA™ Electrocompetent Cells are an altered strain of bacteria, exhibiting Electro competence, making them suitable for subsequent genetic transformation. Their intrinsic antibiotic resistance (in this case not specified), aids cell selection after a complete and successful transformation.


Cell Strain, Genotype and Transformation Efficiency


These VWR International competent cells are a defined genetic strain of bacteria and have a transformation efficiency of high. Their genotype is: explicit.


Methods & Applications


Competent cells enable bacteria to readily take up exogenous DNA, such as a plasmid, which can then either integrate into the cell genome or exist separately as plasmid DNA. After transformation, cells may be used for cDNA library preparation or to amplify the desired segment of DNA through cloning.


BigEasy®-TSA™ Electrocompetent Cells have general applications in transformation but are tailored by VWR International for methods described by the manufacturer below.


Competent Cell Troubleshooting Tips


Competent cells do not transform well if stored at an incorrect temperature; therefore ensure recommended storage conditions of the temperature stated in the protocol. suitable conditions shipping also helps to preserve their transformation efficiency (high) - which can be tested with a standard control plasmid, such as pUC19.

For research use only.