BL21(DE3) Chemically Competent Cell – TransGen Biotech
TransGen Biotech’s BL21(DE3) Chemically Competent Cell are an altered strain of bacteria, exhibiting Chemical competence, making them suitable for subsequent genetic transformation. Their intrinsic antibiotic resistance (in this case not specified), aids cell selection after a complete and successful transformation.
Cell Strain, Genotype and Transformation Efficiency
These TransGen Biotech competent cells are a defined genetic strain of bacteria and have a transformation efficiency of high. Their genotype is: explicit.
Methods & Applications
Competent cells enable bacteria to readily take up exogenous DNA, such as a plasmid, which can then either integrate into the cell genome or exist separately as plasmid DNA. After transformation, cells may be used for cDNA library preparation or to amplify the desired segment of DNA through cloning.
BL21(DE3) Chemically Competent Cell have general applications in transformation but are tailored by TransGen Biotech for methods described by the manufacturer below.
Competent Cell Troubleshooting Tips
Competent cells do not transform well if stored at an incorrect temperature; therefore ensure recommended storage conditions of the temperature stated in the protocol. Dry ice shipping also helps to preserve their transformation efficiency (high) - which can be tested with a standard control plasmid, such as pUC19.