Variant Details
Catalog ID Vendor Catalog ID
Size 500 µl Price $ 22.31
Vendor TransGen Biotech Package Content BL21(DE3) Chemically Competent Cell
Competence Chemical Shipping Condition Dry ice
Storage & Handling -20 °C Expiry Time 6 months
Research Topics Molecular biology


BL21(DE3) Chemically Competent Cell – TransGen Biotech

TransGen Biotech’s BL21(DE3) Chemically Competent Cell are an altered strain of bacteria, exhibiting Chemical competence, making them suitable for subsequent genetic transformation. Their intrinsic antibiotic resistance (in this case not specified), aids cell selection after a complete and successful transformation.

Cell Strain, Genotype and Transformation Efficiency

These TransGen Biotech competent cells are a defined genetic strain of bacteria and have a transformation efficiency of high. Their genotype is: explicit.

Methods & Applications

Competent cells enable bacteria to readily take up exogenous DNA, such as a plasmid, which can then either integrate into the cell genome or exist separately as plasmid DNA. After transformation, cells may be used for cDNA library preparation or to amplify the desired segment of DNA through cloning.

BL21(DE3) Chemically Competent Cell have general applications in transformation but are tailored by TransGen Biotech for methods described by the manufacturer below.

Competent Cell Troubleshooting Tips

Competent cells do not transform well if stored at an incorrect temperature; therefore ensure recommended storage conditions of the temperature stated in the protocol. Dry ice shipping also helps to preserve their transformation efficiency (high) - which can be tested with a standard control plasmid, such as pUC19.

For research use only.