Cathepsin L (CTSL, EC 220.127.116.11) is a lysosomal cysteine protease that is implicated in protein degradation, arthritis, apoptosis and cancer. Intended Use:This simple and high-throughput adaptable assay kit can be used to screen, study and characterize potential inhibitors of Cathepsin L.Test Principle:The Cathepsin L Inhibitor Screening BioAssay™ Kit utilizes the ability of active Cathepsin L to cleave the synthetic AFC-based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin L inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence.Kit Components:CTSL Assay Buffer, 1x15ml CTSL Reagent, 1x0.1ml Cathepsin L (human), 20mU, 1x10ul CTSL Substrate, Ac-FR-AFC, 10mM, 1x0.2ml CTSL Inhibitor, FF-FMK, 1mM, 1x20ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Catalog ID||219614-100RXN||Supplier Catalog ID||219614-100Tests|
|Size||100 Reactions||Price||$ 867.00|
|Supplier||United States Biological Inc.||Package Content||Cathepsin L, Inhibitor Screening BioAssay™ Kit (Fluorometric) (CTSL), 100Tests|
|Shipping Temperature||RT||Storage & Handling||-20°C|
For research use only.