Specifications

Variant Details
Catalog ID219614-100RXNSupplier Catalog ID219614
Size100 ReactionsPrice $ 841.00
Supplier United States Biological Inc. Package Content Cathepsin L, Inhibitor Screening BioAssay™ Kit (Fluorometric) (CTSL), 100Tests
General
Shipping TemperatureRTStorage & Handling-20°C

Description

Cathepsin L (CTSL, EC 3.4.22.15) is a lysosomal cysteine protease that is implicated in protein degradation, arthritis, apoptosis and cancer. Intended Use:This simple and high-throughput adaptable assay kit can be used to screen, study and characterize potential inhibitors of Cathepsin L.Test Principle:The Cathepsin L Inhibitor Screening BioAssay™ Kit utilizes the ability of active Cathepsin L to cleave the synthetic AFC-based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin L inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence.Kit Components:CTSL Assay Buffer, 1x15ml 
CTSL Reagent, 1x0.1ml 
Cathepsin L (human), 20mU, 1x10ul 
CTSL Substrate, Ac-FR-AFC, 10mM, 1x0.2ml 
CTSL Inhibitor, FF-FMK, 1mM, 1x20ulStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only.