Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Sample Type:Adherent and suspension cellsIntended Use:For use in the measurement of cell cytotoxicity in response to drug or toxin treatment, quantification of the cytotoxic effect of immune effector cells on target cells, and assessment of physiological mediators and antibodies that affect cell cytotoxicity.Test Principle:This Cell-Mediated Cytotoxicity BioAssay™ Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.Kit Components:Cytotoxicity Assay Buffer, 2x100ml 7-AAD Staining Solution, 1x0.1ml CFSE Staining Solution, 1x0.1ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Catalog ID||301191-100RXN||Supplier Catalog ID||301191|
|Size||100 Reactions||Price||$ 543.00|
|Supplier||United States Biological Inc.||Package Content||Cell-Mediated Cytotoxicity, Fluorometric BioAssay™ Kit (7-AAD, CFSE), 100Tests|
|Shipping Temperature||RT||Storage & Handling||-20°C|
For research use only.