Specifications

Variant Details
Catalog ID L00339 Vendor Catalog ID L00339
Size 24 Reactions Price $ 175.00
Vendor GenScript Package Content CloneEZ® PCR Cloning Kit
General
Storage & Handling -20°C Expiry Time 1 year

Description

GenScript’s CloneEZ® PCR Cloning Kit


The CloneEZ® PCR Cloning Kit product is part of the GenScript brand. GenScript designed the research kit for experimental processes such as DNA replication and protein expression, but it is also suited to other lab techniques, including those demonstrated in published research papers. CloneEZ® PCR Cloning Kit’s primary application is molecular cloning, but it may also be adjusted to fit corresponding scientific methods.


Downstream Lab Experiments


After use, it may be necessary to carry out further experiments. Relevant research methods for GenScript’s CloneEZ® PCR Cloning Kit include PCR and protein tagging or purification, as well as those stated by the protocol or GenScript.


Troubleshooting Tips: Molecular Cloning


Adhere to the protocol provided by GenScript to ensure that the CloneEZ® PCR Cloning Kit’s components are correctly stored at the temperature noted on the product insert and used within 1 year. By observing these conditions and following protocol instructions as accurately as possible, you will increase the chances of optimum product performance, subsequently improving the likelihood of a successful experiment with this kit.


Discover our broad range of troubleshooting guides applicable to CloneEZ® PCR Cloning Kit, similar reagents, and all valuable Biotech techniques. Each troubleshoot lists the potential problem, cause, and solution; enabling full comprehension of any errors and the required steps to fix them. Get your experiment back on track!


Main Features of this Research Product


-Scientifically developed for molecular cloning, yet is additionally pertinent to the protocols mentioned below.
-Adapted for use in DNA replication and protein expression, and potentially other research methods successfully tested in recent publications.
-Suitable for downstream scientific studies utilizing PCR and protein tagging or purification, or associated techniques.


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Our intelligent search and filters or ‘Alternative Products’ section will help you to find comparable Biotech products to CloneEZ® PCR Cloning Kit. Searching for alternatives to GenScript products? Our integrated compare feature allows cross-brand comparison and will provide you with the essential attributes needed to select the right kit or reagent.


For complete manufacturer information and appropriate research, browse the attached protocols, SDSs, publications, and handbooks located at the bottom of each product page. Is this product missing protocols or other valuable information? Ask our Biotech team to upload them!

For research use only.

Resources

Protocol

TM0279.pdf(pdf)

Publication

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Wang X., et al. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4021-6.

Revilla-Guarinos A., et al. Characterization of a Regulatory Network of Peptide Antibiotic Detoxification Modules in Lactobacillus casei BL23. Appl Environ Microbiol. 2013 May;79(10):3160-70.

Q Wang, et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 2014July;9(7):e102492

Nie C., et al. Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris. Protein Expr Purif. 2013 Nov;92(1):88-93.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto oparticles. Small. 2014April;

Glenn TD., et al. Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin. Development. 2013 Aug;140(15):3167-75.

Dondelinger Y., et al. RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition. Cell Death Differ. 2013 Oct;20(10):1381-92.

Chen Y., et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach. Environ Sci Technol. 2013 Aug 20;47(16):9355-62.

A Elias, et al. Quantitative Comparison of Tumor Delivery for Multiple Targeted oparticles Simultaneously by Multiplex ICP-MS. Sci Rep. 2014July;45840

Article

Protocol

TM0279.pdf(pdf)

Safety

Brochure

Handbook

Publication

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Wang X., et al. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4021-6.

Revilla-Guarinos A., et al. Characterization of a Regulatory Network of Peptide Antibiotic Detoxification Modules in Lactobacillus casei BL23. Appl Environ Microbiol. 2013 May;79(10):3160-70.

Q Wang, et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 2014July;9(7):e102492

Nie C., et al. Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris. Protein Expr Purif. 2013 Nov;92(1):88-93.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto oparticles. Small. 2014April;

Glenn TD., et al. Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin. Development. 2013 Aug;140(15):3167-75.

Dondelinger Y., et al. RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition. Cell Death Differ. 2013 Oct;20(10):1381-92.

Chen Y., et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach. Environ Sci Technol. 2013 Aug 20;47(16):9355-62.

A Elias, et al. Quantitative Comparison of Tumor Delivery for Multiple Targeted oparticles Simultaneously by Multiplex ICP-MS. Sci Rep. 2014July;45840