Specifications

Variant Details
Catalog ID L00339-24RXN Supplier Catalog ID L00339
Size 24 Reactions Price $ 175.00
Supplier GenScript Package Content CloneEZ® PCR Cloning Kit
General
Storage & Handling -20°C Expiry Time 1 year

Description

DescriptionThe GenScript CloneEZ^® PCR Cloning Kit is designed for the quick PCR cloning , it is especially powerful in high-throughput cloning of PCR products into any destination vector effectively by sidestepping the tedious and limiting tasks such as selecting proper restriction enzymes, phosphatases, or ligases. Using our proprietary CloneEZ^® Enzyme, this kit is able to rapidly generate precise and directional constructs by carrying out 30-minute incubation at room temperature (20 °C-25 °C).

For research use only.

Resources

Protocol

TM0279.pdf(pdf)

Publication

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.

Identification of MiR-21-5p as a Functional Regulator of Mesothelin Expression Using MicroRNA Capture Affinity Coupled with Next Generation Sequencing.

Screening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains.

Lipid droplet and peroxisome biogenesis occur at the same ER subdomains.

Generation of H7N9-specific human polyclonal antibodies from a transchromosomic goat (caprine) system.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Wang X., et al. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4021-6.

Revilla-Guarinos A., et al. Characterization of a Regulatory Network of Peptide Antibiotic Detoxification Modules in Lactobacillus casei BL23. Appl Environ Microbiol. 2013 May;79(10):3160-70.

Q Wang, et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 2014July;9(7):e102492

Nie C., et al. Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris. Protein Expr Purif. 2013 Nov;92(1):88-93.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto oparticles. Small. 2014April;

Glenn TD., et al. Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin. Development. 2013 Aug;140(15):3167-75.

Dondelinger Y., et al. RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition. Cell Death Differ. 2013 Oct;20(10):1381-92.

Chen Y., et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach. Environ Sci Technol. 2013 Aug 20;47(16):9355-62.

A Elias, et al. Quantitative Comparison of Tumor Delivery for Multiple Targeted oparticles Simultaneously by Multiplex ICP-MS. Sci Rep. 2014July;45840

Article

Protocol

TM0279.pdf(pdf)

Safety

Brochure

Handbook

Publication

Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.

Identification of MiR-21-5p as a Functional Regulator of Mesothelin Expression Using MicroRNA Capture Affinity Coupled with Next Generation Sequencing.

Screening for inhibitors of mutacin synthesis in Streptococcus mutans using fluorescent reporter strains.

Lipid droplet and peroxisome biogenesis occur at the same ER subdomains.

Generation of H7N9-specific human polyclonal antibodies from a transchromosomic goat (caprine) system.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Wang X., et al. Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):4021-6.

Revilla-Guarinos A., et al. Characterization of a Regulatory Network of Peptide Antibiotic Detoxification Modules in Lactobacillus casei BL23. Appl Environ Microbiol. 2013 May;79(10):3160-70.

Q Wang, et al. Analyzing Cold Tolerance Mechanism in Transgenic Zebrafish (Danio rerio). PLoS One. 2014July;9(7):e102492

Nie C., et al. Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris. Protein Expr Purif. 2013 Nov;92(1):88-93.

Liu Y, et al. Enhancement of thermo-stability and product tolerance of Pseudomonas putida nitrile hydratase by fusing with self-assembling peptide. J Biosci Bioeng. 2014Mar.;

Hui JZ, et al. Facile Method for the Site‐Specific, Covalent Attachment of Full‐Length IgG onto oparticles. Small. 2014April;

Glenn TD., et al. Analysis of Gpr126 function defines distinct mechanisms controlling the initiation and maturation of myelin. Development. 2013 Aug;140(15):3167-75.

Dondelinger Y., et al. RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition. Cell Death Differ. 2013 Oct;20(10):1381-92.

Chen Y., et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach. Environ Sci Technol. 2013 Aug 20;47(16):9355-62.

A Elias, et al. Quantitative Comparison of Tumor Delivery for Multiple Targeted oparticles Simultaneously by Multiplex ICP-MS. Sci Rep. 2014July;45840