Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.2,3 Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.4,5
3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one; CLZN; Preluciferin; Luciferin; 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one
Supplied as a brownish/yellow to yellow/orange crystalline powder
Solubility (0.0125mg/ml in methanol:
Orange to yellow to brown liquid
Molar Extinction Coefficient (0.0125mg/ml in methanol, lambda max1):
>7800.0 L/(mol cm)
UV (0.0125mg/ml in methanol, lambda max1):
UV (0.0125mg/ml in methanol, lambda max2):
Storage and Stability:
Store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic and light sensitive. Store in amber vial under inert atmosphere.
Note: It is not recommended to store dissolved coelenterazine at -20°C or -70°C. Coelenterazine is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.
PubChem Chemical ID: 2830
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards:
All products should be handled by qualified personnel only, trained in laboratory procedures.