Specifications

Variant Details
Catalog IDE009Supplier Catalog IDE009
Size25 ReactionsPrice $ 65.00
Supplier Applied Biological Materials Inc. Package Content Klenow Fragment (3’→5’ Exo-) 5 U/μl, 100 µl 10X dA-Tailing Reaction Buffer, 200 µl
General
Sequencing TypeNext Generation sequencingStarting MaterialdsDNA
Time to Sample45 minAmount of Starting Material1-5 µg end repaired, blunt-ended DNA
Specialized ApplicationDNA sample preparation, dA-Tailing of 1-5 μg of blunt ended DNAShipping ConditionDry ice
Storage & Handling-20 °CShelf Life12 months
Research TopicsMolecular biology

Description

The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3’ end of a blunt-ended DNA fragment. This incorporated 3’-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3’-dT overhangs. The Klenow Fragment (3´→ 5´ Exo-) in this kit adds the 3’-dA via its DNA polymerase activity. This enzyme lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity, thus preventing degradation of this 3’-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3´→ 5´ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038).

For research use only.

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Protocol

DNA dA-Tailing Kit

Article

Protocol

DNA dA-Tailing Kit

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