Specifications

Variant Details
Catalog IDE015Supplier Catalog IDE015
Size2000 UnitsPrice $ 15.00
Supplier Applied Biological Materials Inc. Package Content Exonuclease I, E. coli, 100 µl 10X Exonuclease I Reaction Buffer, 500 µl
General
Enzyme TypeExonucleaseEnzyme Concentration20 U/µl
Unit DefinitionOne unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37 °C in 1X Exonuclease I Reaction BufferTemplatesssDNA
Thermal Inactivation20 min at 80 °CProduct ApplicationRemoval of nucleotides from single-stranded DNA in the 3’→5’ direction
Shipping ConditionDry iceStorage & Handling-20 °C
Shelf Life12 monthsResearch TopicsMolecular biology

Description

Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.

For research use only.

Resources

Protocol

Exonuclease I, E.coli

Article

Protocol

Exonuclease I, E.coli

Safety

Brochure

Handbook

Publication

Validation