FastStart SYBR Green Master for Real-Time PCR – Roche Life Science
Roche Life Science’s FastStart SYBR Green Master is designed for qPCR (quantitative polymerase chain reaction) with Genomic DNA, Plasmid DNA, cDNA, and other tested templates, via thermal cycling, and a dedicated FastStart Taq DNA Polymerase.
Due to Roche Life Science protocol and components, this research kit has a regular and Singleplex classification.
Real-Time PCR Reaction Volume & Amplicon Size
Amplicons of < 500 bp can be produced from < 250 ng genomic DNA, < 50 ng cDNA, as part of a 20 µl reaction volume. These specifications are facilitated by Roche Life Science’s included enzyme: FastStart Taq DNA Polymerase, with an activation requirement of appropriate time and temperature.
This Roche Life Science Real-time PCR Kit additionally contains the fluorescent reporter SYBR Green I, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the All real-time PCR instruments using the SYBR Green I detection format (except the LightCycler Instruments), and other specified instruments.
Applications & Downstream Experiments
The FastStart SYBR Green Master is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.
qPCR Troubleshooting Tips
Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.