Specifications

Variant Details
Catalog ID786-1040Supplier Catalog ID786-1040
Size5 mlPrice $ 69.00
Supplier G-Biosciences, US Package Content G-Sep™ NI NTA Agarose Fast Flow (FF) resin
General
TargetsProteinsSize RangeExclusion limit(globular proteins): 4 x 106
Specialized ApplicationPurification of recombinant proteins fused to the 6x histidine (6XHis) tag.Storage & Handling2 to 8°C, 20% Ethanol

Description

Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. G-Sep™NI NTA Agarose Fast Flow is specifically designed for the purification of recombinant proteins fused to the 6x histidine (6XHis) tag. The resin is specifically designed for the purification of recombinant proteins fused to the 6X histidine (6XHis) tag expressed in bacteria, insects, and mammalian cells. The resin is high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. G-Sep™ NI NTA Agarose Fast Flow (FF) resin is nickel ions immobilized onto highly cross-linked 6% agarose beads. The G-Sep™ NTA Agarose Fast Flow (FF) resins have high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. G-Sep™ Ni NTA Agarose Fast Flow (FF) resin using cobalt is also available

Features

Matrix: Cross-linked agarose beads, 6%
Bead form: Spherical, diameter 50-160μm
Spacer: Epichlorohydrin
Chelating Agent: nitrilotriacetic acid (NTA)
pH stability Working Range: 3-12
pH stability Cleaning-in-Place (CIP): 2-14
Maximum Flow Velocity: 450cm/h
Physical Stability: Negligible volume variation due to changes in pH or ionic strength
Chemical Stability: Stable to all commonly used aqueous buffers: 6M urea, 8M guanidine hydrochloride,
Autoclavable: 121°C, pH 7, for 30 min

For research use only.