Description

GenElute™ FFPE RNA Purification Kit for Total RNA, mRNA, miRNA, siRNA Extraction - Sigma-Aldrich


Sigma-Aldrich’s GenElute™ FFPE RNA Purification Kit is designed for extracting Total RNA, mRNA, miRNA, siRNA and excluding unwanted impurities. Compatible inputs include FFPE tissue, and equivalent sample types. Such inputs must originate from Animals, Human or published organisms. Specific sub-species include those mentioned in the product insert and their close relatives. For further information, see Sigma-Aldrich specifications below.


Efficient RNA isolation and optimal yield


GenElute™ FFPE RNA Purification Kit’s tailored protocol, Spin column chromatography, and Not specified kit format all facilitate its key lab applications. According to Sigma-Aldrich, these are; Isolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). For optimal results, the initial sample amount should be at least 20 µm FFPE tissue section (25 mg unsectioned core). Ideal final concentration is attainable with an elution volume of 20-50 µl, (i.e., elution buffer).


Applicable Methods/Experiments


The purified RNA sample is usable in the following methods: Expression array assays, Microarray analysis, Primer extension, RT-PCR, RT-qPCR, and other associated analyses.

For research use only.

Specifications

Single/Multiple Target KitsRNA
Column SizeNot specified
Sample TypeFFPE tissue
Sample Amount20 µm FFPE tissue section (25 mg unsectioned core)
TargetsTotal RNA, mRNA, miRNA, siRNA
OrganismAnimals, Human
TechnologySpin column chromatography
Elution Volume20-50 µl
Time to Sample< 60 min
Product ApplicationIsolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
Downstream ProcessesExpression array assays, Microarray analysis, Primer extension, RT-PCR, RT-qPCR
ProcessingManual
Shipping ConditionWet ice
Storage Temperature-20 °C, 2-8 °C
Expiry Time24 months
Package Content Digestion Buffer A Buffer RL Enzyme Incubation Wash Solution A Elution Solution A Proteinase K DNase I Micro Spin Columns Collection Tubes Elution tubes (1.7 mL) Product Insert
Variant Details Size: 50 Preparations Format Non-Plated Catalog ID RNB400 Price $ 350.00

Resources

Protocol

GenElute™ FFPE RNA Purification Kit
www.sigmaaldrich.com PDF