GenElute™ FFPE RNA Purification Kitby Sigma-Aldrich
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GenElute™ FFPE RNA Purification Kit for miRNA, mRNA, siRNA, Total RNA Extraction - Sigma-Aldrich
Sigma-Aldrich’s GenElute™ FFPE RNA Purification Kit is designed for extracting miRNA, mRNA, siRNA, Total RNA and excluding unwanted impurities. Compatible inputs include FFPE tissue, and equivalent sample types. Such inputs must originate from Animals, Human, or published organisms. Specific sub-species include those mentioned in the product insert and their close relatives. For further information, see Sigma-Aldrich specifications.
Efficient RNA isolation and optimal yield
GenElute™ FFPE RNA Purification Kit’s tailored protocol, Spin column chromatography, and Not specified kit format all facilitate its key lab applications. According to Sigma-Aldrich, these are; Isolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). For optimal results, the initial sample amount should be at least 20 µm FFPE tissue section (25 mg unsectioned core). Ideal final concentration is attainable with an elution volume of 20-50 µl, (i.e., elution buffer).
The purified RNA sample is usable in the following methods: RT-qPCR, RT-PCR, Primer extension, Expression array assays, Microarray analysis, and other associated analyses.