Description

GenElute™ FFPE RNA Purification Kit for miRNA, mRNA, siRNA, Total RNA Extraction - Sigma-Aldrich


Sigma-Aldrich’s GenElute™ FFPE RNA Purification Kit is designed for extracting miRNA, mRNA, siRNA, Total RNA and excluding unwanted impurities. Compatible inputs include FFPE tissue, and equivalent sample types. Such inputs must originate from Animals, Human, or published organisms. Specific sub-species include those mentioned in the product insert and their close relatives. For further information, see Sigma-Aldrich specifications.


Efficient RNA isolation and optimal yield


GenElute™ FFPE RNA Purification Kit’s tailored protocol, Spin column chromatography, and Not specified kit format all facilitate its key lab applications. According to Sigma-Aldrich, these are; Isolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). For optimal results, the initial sample amount should be at least 20 µm FFPE tissue section (25 mg unsectioned core). Ideal final concentration is attainable with an elution volume of 20-50 µl, (i.e., elution buffer).


Applicable Methods/Experiments


The purified RNA sample is usable in the following methods: RT-qPCR, RT-PCR, Primer extension, Expression array assays, Microarray analysis, and other associated analyses.

For research use only.

Specifications

Single/Multiple Target KitsRNA
Extraction ofmiRNA, mRNA, siRNA, Total RNA
Sample TypeFFPE tissue
OrganismAnimals, Human
TechnologySpin column chromatography
Time to Sample< 60 min
Kit SizeNot specified
Low Hazard ProductYes
Sample Amount20 µm FFPE tissue section (25 mg unsectioned core)
Elution Volume20-50 µl
Product ApplicationIsolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
Downstream ProcessesRT-qPCR, RT-PCR, Primer extension, Expression array assays, Microarray analysis
ProcessingManual
Shipping ConditionWet ice
Storage Temperature2-8 °C, -20 °C
Expiry Time24 months
Package Content Digestion Buffer A Buffer RL Enzyme Incubation Wash Solution A Elution Solution A Proteinase K DNase I Micro Spin Columns Collection Tubes Elution tubes (1.7 mL) Product Insert
Variant Details Size: 50 Preparations Format Non-Plated Catalog ID RNB400 Price $ 350.00

Resources

Protocol

GenElute™ FFPE RNA Purification Kit
www.sigmaaldrich.com PDF

Discussion