Description

Sigma’s GenElute™ FFPE RNA Purification Kit provides a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Sigma’s GenElute™ FFPE RNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including qRT-PCR, reverse transcription PCR, primer extension, expression array assays, and microarray analyses.

• Extract total RNA (including microRNA) from FFPE samples
• No phenol extraction step
• Includes DNase for optional on-column DNA removal
• Isolated RNA is of the highest quality and integrity

Specifications

Single/Multiple Target KitsRNA
Extraction ofmiRNA, mRNA, siRNA, Total RNA
Sample TypeFFPE tissue
OrganismAnimals, Human
TechnologySpin column chromatography
Yield/
Time to Sample< 60 min
Kit SizeNot specified
Low Hazard ProductYes
Sample Amount20 µm FFPE tissue section (25 mg unsectioned core)
Elution Volume20-50 µl
Product ApplicationIsolate a diversity of RNA species from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
Downstream ProcessesRT-qPCR, RT-PCR, Primer extension, Expression array assays, Microarray analysis
ProcessingManual
Shipping ConditionWet ice
Storage Temperature2-8 °C, -20 °C
Expiry Time24 months
Package Content Digestion Buffer A Buffer RL Enzyme Incubation Wash Solution A Elution Solution A Proteinase K DNase I Micro Spin Columns Collection Tubes Elution tubes (1.7 mL) Product Insert
Variant Details Size: 50 Preparations Format Non-Plated Catalog ID RNB400 Price $ 350.00

Alternative Products

FFPE RNA Purification Kit Norgen Biotek
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Resources

Protocol

GenElute™ FFPE RNA Purification Kit protocol
www.sigmaaldrich.com PDF

Safety

Discussion