Golden Taq Polymeraseby ProteoGenix
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Golden Taq Polymerase for End-Point PCR – ProteoGenix
ProteoGenix’s Golden Taq Polymerase utilizes the enzyme Golden Taq Polymerase, to amplify Genomic DNA, Plasmid DNA, cDNA, and similar templates. Included PCR reagents and buffers help support regular PCR in addition to singleplex PCR, allowing complete control over amplification specificity (i.e., specific or non-specific amplification) and complexity (single or multiple target amplification).
Polymerase and Protocol Specifications
The Golden Taq Polymerase is supplied at 5 U/µl and is activated by conditions stated in the protocol during denaturing. Following annealing, nucleic acid samples of 10-100 ng PCR products are elongated at 72 °C. ProteoGenix recommends a 30 µl PCR reaction volume and amplicons of up to 5000bp for optimal polymerase performance. Utilizing thermal cycling and regular PCR to replicate DNA, Golden Taq Polymerase extends primers with free dNTPs, forming a complementary second strand.
Applications & Downstream Experiments of Golden Taq Polymerase
Intended uses of ProteoGenix’s Golden Taq Polymerase include For routine PCR and RT-PCR, as well as related PCR cloning and amplification techniques. The resulting product is applicable for use in Cloning, and other tested methods.
PCR Troubleshooting Tips
Contamination and inappropriate primer construction create amplification inefficiencies. Therefore, PCR cleanup kits enhance the purity of either the starting or final sample, maximizing accuracy, and effectiveness. Dedicated primer design tools may also be useful in those respects. Optimum temperatures suggested by Invitrogen should be enforced, e.g., elongation (72 °C). Fidelity and enzyme concentration (5 U/µl) also need to be accounted for when setting up your PCR.