Description

The Golden Taq Polymerase, from the hemophilic bacteria Thermus aquaticus is a highly thermostable DNA polymerase used for standard PCR applications in order to amplify DNA sequences. The recombinant form of this enzyme expressed in bacterial expression system (E.coli) has a molecular weight of ~95 kDa. Reaction buffer and an MgSO4 solution are included for possible optimizations.

Concentration: 5 U / μl

For research use only.

Specifications

PCR TypeOne-step PCR
TemplatescDNA, Genomic DNA, Plasmid DNA
Template Amount100-200 ng genomic DNA; 10-100 ng plasmid DNA, cDNA, PCR fragments
PolymeraseGolden Taq Polymerase
Amplicon Sizeup to 5000bp
Reaction Volume30 µl
Sample TypePCR products
Sample Amount10-100 ng PCR products
OrganismBacteria
Enzyme Concentration5 U/µl
Elongation Temperature72 °C
Unit DefinitionOne unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into a DNA fragment in 30 minutes at 74°C
Concentration10X
Product ApplicationFor routine PCR and RT-PCR
Downstream ProcessesCloning
Shipping Temperature-20 °C
Storage Temperature-20 °C
Expiry Time12 months
Low Hazard ProductYes
LyophilizedLyophilized
Research TopicsMolecular biology
Package Content Taq DNA Polymerase, 50 µl 10X reaction buffer, 250 µl MgSO4 (100mM), 200 µl
Variant Details Size: 250 Units Format Non-Plated Catalog ID PI-MB001-250 Price $ 36.37

Resources

Protocol

Golden Taq Polymerase (FR)
www.proteogenix-products.com PDF
Golden Taq Polymerase Datasheet (EN)
www.proteogenix-products.com PDF

Discussion