HighPrep DTR is a high performance paramagnetic bead-based system, designed to remove unincorporated dye terminators from Sanger sequencing reaction. The HighPrep DTR system consists of a selective binding of DNA to the HighPrep DTR paramagnetic beads, followed with washing off nucleotides, primers and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR is designed for both manual and fully automated purification of sequencing products. The protocol can be adapted to your current lqiuid handling workstation (e.g. Beckman, Hamilton, Tecan, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol.
Features & Benefits
Long Phred20 read lengths averaging over 700 bps
Pass rates over 85% or higher
Increased average signal strength
Efficient clean up of sequencing reaction contaminants
Reduce BigDye usage