Specifications

Variant Details
Catalog IDE-2017-3Supplier Catalog IDE-2017-3
Size250 UnitsPrice $ 93.60
Supplier Bioneer Corp. Package Content HotStart Taq DNA polymerase 250 U 10X reaction buffer w/ MgCl2 0.5 ml Dilution buffer 0.5 ml Instruction manual
General
Enzyme TypePolymeraseEnzyme Concentration5 U/µl
TemplatesGenomic DNA, Plasmid DNA, dsDNATemplate Amount1 pg - 100 ng
SourceE. coliIncubation Temperature72 °C
PolymeraseTaq DNA PolymeraseActivation Step15 min at 94 °C
Elongation Temperature72 °CAmplicon Size50-10,000 bp
Sample TypeCell-free sample, Lysate, PCR products, Plasma & serum, Purified DNASample Amount5-10 µl
Specialized ApplicationAmplification of DNAApplicationsEnd-point PCR, Real-time PCR
Shipping ConditionDry iceShipping Temperature-70 °C
Storage & Handling-20 °CShelf Life24 months
Research TopicsBiochemistry, Cancer research, Cell biology, Genetics, Molecular biology

Description

HotStart Taq DNA Polymerase is designed to increase specificity and sensitivity in PCR. The HotStart Taq DNA polymerase is inhibited at temperatures lower than 70°C, but is fully activated after the first denaturation step. This prevents the formation of mis-primed products and primer-dimers during the reaction setup process, resulting in improved PCR specificity.

For research use only.

Resources

Protocol

HotStart Taq DNA Polymerase

Article

Protocol

HotStart Taq DNA Polymerase

Brochure

Handbook

Publication

Validation