Specifications

Variant Details
Catalog IDAE40362HU-48TSupplier Catalog IDAE40362HU
Size48 ReactionsPrice $ 325.00
Supplier Abebio, US (Wuhan Abebio Science Co.,Ltd.) Package Content Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1) ELISA Kit
General
ELISA TypeSandwichTarget MoleculePotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 (HCN1)
Sample TypeBiological fluids, Plasma & SerumReactivityHuman
Detection Range78.1-5000 pg/mLUniProtKBO60741

Description

Test principle
This assay employs a two-site sandwich ELISA to quantitate HCN1 in samples. An antibody specific for HCN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HCN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).

For research use only.