Specifications

Variant Details
Catalog ID219905-25UGSupplier Catalog ID219905
Size25 µgPrice $ 403.00
Supplier United States Biological Inc. Package Content IL-17A, Recombinant, Human (Interleukin-17A, IL-17, IL-17A, Cytotoxic T-Lymphocyte-Associated Antigen 8, CTLA-8, CTLA8, IL17), 25ug
General
SourceRecombinant, E. coliReconstitutionReconstitute with sterile ddH2O to 0.1-1mg/ml.
Purity~98% (SDS-PAGE, HPLC)FormLyophilized, Powder
Additional InformationSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.Shipping TemperatureBlue Ice
Storage & Handling-20°CPurification MethodHighly Purified
Swiss-ProtQ16552

Description

The original described IL-17 protein, now known as IL-17A, was identified from a CD4+ T cells DNA library. IL-17 can be induced from primary peripheral blood CD4+ T cells upon stimulation. Supernatant from cells transfected with IL-17 induced IL-16 and IL-18 production and enhanced the surface expression of the intraCellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Source:Recombinant protein corresponding to human IL-17A, a disulfide-linked homodier of two 136aa polypeptide chains, expressed in E. coli. Molecular Weight: ~31kDBiological Activity:The ED50, as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts, is <2ng/ml, corresponding to a specific activity of >5x10e5units/mg.Endotoxin:<0.1ng/ugStorage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.