Specifications

Variant Details
Catalog IDMAK025-1KTSupplier Catalog IDMAK025-1KT
Size100 ReactionsPrice $ 447.00
Supplier Sigma-Aldrich, Inc. Package Content Iron Assay Buffer, 25 ml Iron Probe, 12 ml Iron Reducer, 0.7 ml Iron Standard, 100 mM, 0.1 ml
General
Control TypeStandardQuantification MethodComplex building
TargetsMetaboliteAbsorbance593 nm
Detection TechniqueColorimetryProduct ApplicationMeasuring iron in a variety of biological samples
Shipping ConditionWet iceStorage & Handling-20 °C
Research TopicsCell biology

Description

Iron is a mineral that plays an essential role in many biological processes, including iron transport and redox reactions. Iron is a transition element that can form a range of oxidation states, the most common being
iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.

In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.

For research use only.

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