Specifications

Variant Details
Catalog ID 95059-200 Vendor Catalog ID 101414-180
Size 200 Assays Price $ 638.28
Vendor VWR International, LLC Package Content Kit One Step Qrt-Pcr Low Rox 200Rxn

Description

Kit One Step Qrt-Pcr Low Rox 200Rxn for Real-Time PCR – Quantabio


Quantabio’s Kit One Step Qrt-Pcr Low Rox 200Rxn is designed for qPCR (quantitative polymerase chain reaction) with specified nucleic acids, and other tested templates, via thermal cycling, and a dedicated polymerase.
Due to Quantabio protocol and components, this research kit has a regular and Singleplex classification.


Real-Time PCR Reaction Volume & Amplicon Size


Amplicons of varying size can be produced from the stated amount of nucleic acid template, as part of a recommended reaction volume. These specifications are facilitated by Quantabio’s included enzyme: polymerase, with an activation requirement of appropriate time and temperature.
This Quantabio Real-time PCR Kit additionally contains the fluorescent reporter specified in the product insert, with an applicable reporter label included as a corresponding reference dye. As a result, this research product is compatible with the majority of qPCR systems, and other specified instruments.


Applications & Downstream Experiments


The Kit One Step Qrt-Pcr Low Rox 200Rxn is suitable for gene expression analyses, among other research experiments and is compatible with electrophoresis or other tested methods.


qPCR Troubleshooting Tips


Quantitative PCR amplification problems can persist due to incorrect primer design or contamination of the sample. However, it is also vital to ensure each stage is performed at optimum temperature during annealing and elongation phases (stated in the protocol). Furthermore, enzyme concentration (available from the manufacturer) and fidelity should be considered when determining experiment processes and conditions.

For research use only.